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The map label for this gene is ilvE [H]

Identifier: 161870733

GI number: 161870733

Start: 1851286

End: 1852236

Strand: Direct

Name: ilvE [H]

Synonym: NMCC_1806

Alternate gene names: 161870733

Gene position: 1851286-1852236 (Clockwise)

Preceding gene: 161870732

Following gene: 161870737

Centisome position: 85.97

GC content: 56.99

Gene sequence:

>951_bases
ATGCCCGTCCTCGCCTACCGCGAAGGCAAATGGCAGCCGACCGAATGGCAATCTTCCCAAGACCTCTCCCTCGTACCGGG
CGCGCACGCCCTGCACTACGGCAGCGAATGTTTCGAGGGACTGAAAGCCTTCCGTCAGGCAGACGGTAAAATCGTACTGT
TCCGTCCGACTGCCAATATCGCGCGTATGCGGCAAAGTGCGGACATTTTGCACCTGCCCCGCCCCGAAACCCAAGCCTAT
CTCGATGCTTTGGTCGAACTGGTCAAACGTGCCGCCGACGAAATTCCCGATGCGCCTGCCGCCCTGTACCTGCGCCCGAC
CTTAATCGGTACCGATCCCGTTATCGGCAAGGCCGGTTCTCCTTCCGAAACCGCCCTGCTGTATATTTTGGCTTCCCCCG
TCGGCGACTATTTCAAAGTCGGCTCGCCCGTCAAAATTTTAGTGGAAACCGAACACATCCGCTGCGCCCCGCATATGGGC
CGCGTCAAATGCGGCGGCAACTACGCTTCCGCCATGCACTGGGTGCTGAAGGCGAAAGCCGAATACGGCGCAAACCAAGT
CCTCTTCTGCCCGAACGGCGACGTGCAGGAAACCGGCGCGTCCAACTTTATCCTGATTAACGGCGATGAAATCATTACCA
AACCGCTGACCGACGAGTTTTTGCACGGCGTTACCCGCGATTCCGTGCTGACGGTTGCCAAAGATTTGGGCTATACCGTC
AGCGAACGCAATTTCACGGTTGCCGAACTGAAAGCTGCGGTGGAAAACGGTGCGGAGGCGATTTTGACCGGTACGGCCGC
GGTCATTTCTCCGGTAACTTCTTTCGTGATTGACGGCAAAGAAATCGAAGTGAAAAGCCAAGAACGCGGCTACGCCATCC
GTAAGGCGATTACCGACATCCAGTATGGTTTGGCGGAAGACAAATACGGCTGGCTGGTTGAAGTGTGCTGA

Upstream 100 bases:

>100_bases
TCAAATTCAAACCGTTCATTGAAACATATCAGAATAAGAAAGGCTTTACATCATGAGCAGACCCGTACCCGCCGTATTCG
GCAGCGTTTTTCACAGTCAA

Downstream 100 bases:

>100_bases
TGCTTTAAATAAAAAAATGCCGTCTGAAACCCGTTTGGCGTTTCAGACGGCATTTTCGCGTTCGGACCATTTCCGCCGCA
CCTGCAGCAAGTCGGCATAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPVLAYREGKWQPTEWQSSQDLSLVPGAHALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAY
LDALVELVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPVKILVETEHIRCAPHMG
RVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASNFILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTV
SERNFTVAELKAAVENGAEAILTGTAAVISPVTSFVIDGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC

Sequences:

>Translated_316_residues
MPVLAYREGKWQPTEWQSSQDLSLVPGAHALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAY
LDALVELVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPVKILVETEHIRCAPHMG
RVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASNFILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTV
SERNFTVAELKAAVENGAEAILTGTAAVISPVTSFVIDGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC
>Mature_315_residues
PVLAYREGKWQPTEWQSSQDLSLVPGAHALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYL
DALVELVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPVKILVETEHIRCAPHMGR
VKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASNFILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVS
ERNFTVAELKAAVENGAEAILTGTAAVISPVTSFVIDGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=320, Percent_Identity=39.0625, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI296010906, Length=320, Percent_Identity=39.0625, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI38176287, Length=320, Percent_Identity=39.0625, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI50658084, Length=327, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI258614015, Length=292, Percent_Identity=33.9041095890411, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI296010902, Length=320, Percent_Identity=32.8125, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI296010900, Length=320, Percent_Identity=32.8125, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI48994963, Length=311, Percent_Identity=27.9742765273312, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17568601, Length=332, Percent_Identity=36.7469879518072, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17565728, Length=313, Percent_Identity=34.5047923322684, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322608, Length=321, Percent_Identity=35.8255451713396, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6322002, Length=305, Percent_Identity=38.6885245901639, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24641779, Length=324, Percent_Identity=37.3456790123457, Blast_Score=181, Evalue=7e-46,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34389; Mature: 34257

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLAYREGKWQPTEWQSSQDLSLVPGAHALHYGSECFEGLKAFRQADGKIVLFRPTANI
CCEEECCCCCCCCCCCCCCCCCEECCCCHHHHCCHHHHHHHHHHHHCCCEEEEEECCHHH
ARMRQSADILHLPRPETQAYLDALVELVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGS
HHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCC
PSETALLYILASPVGDYFKVGSPVKILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKA
CCCCEEEEEEECCCCHHHHCCCCEEEEEEECCEEECCCCCCEEECCCHHHHHHHHHHHHH
EYGANQVLFCPNGDVQETGASNFILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTV
HCCCCEEEECCCCCCCCCCCCCEEEECCCCEEECCCHHHHHHCCCHHHHHHHHHHCCCEE
SERNFTVAELKAAVENGAEAILTGTAAVISPVTSFVIDGKEIEVKSQERGYAIRKAITDI
CCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHEECCCEEEECCCCCCHHHHHHHHHH
QYGLAEDKYGWLVEVC
HHCCCCCCCCEEEEEC
>Mature Secondary Structure 
PVLAYREGKWQPTEWQSSQDLSLVPGAHALHYGSECFEGLKAFRQADGKIVLFRPTANI
CEEECCCCCCCCCCCCCCCCCEECCCCHHHHCCHHHHHHHHHHHHCCCEEEEEECCHHH
ARMRQSADILHLPRPETQAYLDALVELVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGS
HHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCC
PSETALLYILASPVGDYFKVGSPVKILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKA
CCCCEEEEEEECCCCHHHHCCCCEEEEEEECCEEECCCCCCEEECCCHHHHHHHHHHHHH
EYGANQVLFCPNGDVQETGASNFILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTV
HCCCCEEEECCCCCCCCCCCCCEEEECCCCEEECCCHHHHHHCCCHHHHHHHHHHCCCEE
SERNFTVAELKAAVENGAEAILTGTAAVISPVTSFVIDGKEIEVKSQERGYAIRKAITDI
CCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHEECCCEEEECCCCCCHHHHHHHHHH
QYGLAEDKYGWLVEVC
HHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]