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The map label for this gene is pilD [H]

Identifier: 161870738

GI number: 161870738

Start: 1857960

End: 1858820

Strand: Direct

Name: pilD [H]

Synonym: NMCC_1811

Alternate gene names: 161870738

Gene position: 1857960-1858820 (Clockwise)

Preceding gene: 161870737

Following gene: 161870739

Centisome position: 86.28

GC content: 53.31

Gene sequence:

>861_bases
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGACAGCGGTGTTCGGGCTGCTGGTCGGAAGTTTCTTAAA
TGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAATATTTAAACCTGCCGCTGA
CCGAAGAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCCTCCTGCCAAACCAAAATCAGCATACGCTATCC
CTTAATCGAACTGCTGACCGGCGTATTGTTCGGGCTGGTTGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Upstream 100 bases:

>100_bases
TTTAATTTGGGCAACGTGGTCGCCTGATTTGCCGCACAGATCCGGCGCGGATTCGCTCTGCGCCGGTTTGTTTTTGTTTT
GAATACATTGAGAACAAAAT

Downstream 100 bases:

>100_bases
GATGACGGTATGGGTCGGACTGACCGGCGGAATCGGCAGCGGCAAATCGGCAGCCGCGCAATGTTTTGCCGATTTGGGCG
TACCGCGTATCGATGCGGAT

Product: leader peptidase (prepilin peptidase) / N-methyltransferase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Sequences:

>Translated_286_residues
MSDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL
>Mature_285_residues
SDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQ
NIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLPL
IWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLVA
AIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=257, Percent_Identity=38.5214007782101, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1789732, Length=141, Percent_Identity=44.6808510638298, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31390; Mature: 31259

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCEECCCCEEECCEEEEECCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRT
CCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCE
FNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLV
ECCCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHH
AWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLPLIWLGLIFNLDGGFVPLQSA
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
VLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL
HHHHHHHHCCCCEECCCCEEECCEEEEECCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8100347; 7906688; 7565116 [H]