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The map label for this gene is agrA [H]

Identifier: 161870303

GI number: 161870303

Start: 1358677

End: 1360218

Strand: Reverse

Name: agrA [H]

Synonym: NMCC_1345

Alternate gene names: 161870303

Gene position: 1360218-1358677 (Counterclockwise)

Preceding gene: 161870307

Following gene: 161870302

Centisome position: 63.17

GC content: 53.83

Gene sequence:

>1542_bases
ATGTTTTTCGATAATAATTATCATTACAATGCAATACCGGTTCGTTTGCTTGTGAACATTCAAGATGCCGACTCTGACGG
CATTCAGACAGCGTCTGAAAACAATAACGGCACAAGGATGGGGTACAATCGCCCTATGGAAAAAAACACCCTATCCGCCC
GCGCACCATCCCCTTGGCTGCCGCTTCTGCTGGCAATTGCCATTTTTATGCAGATGTTGGATGCGACCATTTTAAATACC
GCACTGCCTGAAATTGCCACCGACCTGGACGAATCGCCTCTGGATATGCAACTGGCAGTCGTTGCCTATACGCTGACGGT
TGCCCTGCTGATTCCTTTGAGCGGTTATTTGGCGGACAGGTTCGGAACGAAAAAAGTCTTTTTCGGTTCGATTGCCGTTT
TTATGCTCGGATCGGCATTGTGCGCCGCATCGGGTTCGCTGTTTGAATTGACGCTTTCCCGTGTCGTTCAGGGCATCGGC
GGTTCGATGCTGGTTCCGATACCGCGTCTGACCATCTTGCGTATGTACGACAAGTCCAAGCTGCTCAACGCCATCAATTA
TGCGGTTATGCCCGCATTAATCGGGCCGGTTTTAGGGCCTTTGGCGGGCGGTTATCTGGTCGAATACGCTTCGTGGCACT
GGATTTTCCTGCTCAACCTGCCCATCGGTCTGCTGGGGTTTATATTGGGACGCAACATCATGCCCGATGTCAAAGGCAAC
GATACCGCTCTGGATTTTAAAGGCTATCTGACCTTTTCCGCTGCCGCCTGCCTGCTACTGCTTGCTGCAGAAAGCCTGTC
GCACGCGCTGCCTCCGTATTTTGCACTGTTGCCGCTGTGCGGCGGACTGCTGTTTGCACGCCGTTATTTCCGACATATGA
AAACCACGTCCAAACCGATTTATTCCGCCGACCTGTTTCTGATACGCACTTTCCGTCTGGGACTGGCAGGCAATCTGTTC
AGCCGTCTCGGCATCAGCTCGATTCCTTTTCTGATGCCCCTGATGTTTCAAGTCGCTTTCGGCTTCGGCGCAAGCCTGTC
GGGTTGGCTGGTCGCCCCCGTCGCCCTGTCTTCGCTGCTGGTCAAACCGCTGATTGCACCGCTCATGAAACGTTTCGGCT
ACCGCACGGTACTGCTTTGGAACACCAAGCTGCTTGCCGCCTTCATCATGCTGCTCGCCCTGCCTGACGGAAACTCGTCG
CTGTGGATTTGGATTTTCCTCTCGCTGGCGATCGGCGCGTGCAACTCCCTGCAGTTTTCTGCCATGAATACACTGACCCT
CGCCGATTTGCGCCCGCAACAGACCGGCAGCGGCAACAGCCTGATGGCGGTCAACCAACAGCTTGCCATCAGCATGGGCA
TTGTTGTCGGCGCATTAATCCTTAAAAACTGGACATTTCTGATACCGGCTTCTTCAGGTCTGCATTCCGCCTTCCGTATG
ACCCTGCTCAGCATCGGCGGCATCACCCTTGCCTCATCGCTGGTATTCAATCGGCTGCACGTTTCAGACGGCACCAACCT
GACACGGAATACACCGTCCTGA

Upstream 100 bases:

>100_bases
TTACTTTAAGTAATTGACTGTTGCGGGTCAAGTCTAATTTTAAAAAATAATCCGGTTTTTCTTACAAACTACCCCATAAC
GCTTACTGTACCTTAATCTG

Downstream 100 bases:

>100_bases
AGCGGTCCGCACGCAAAACTTTTACCCGTTTCAACGTTTGGATTATGATGCCGCACTTCCATGCGCACCAACCCCAAAAC
ACAGGCAATGCCGTCTGAAC

Product: efflux transporter membrane protein AgrA

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MFFDNNYHYNAIPVRLLVNIQDADSDGIQTASENNNGTRMGYNRPMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNT
ALPEIATDLDESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSLFELTLSRVVQGIG
GSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGYLVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGN
DTALDFKGYLTFSAAACLLLLAAESLSHALPPYFALLPLCGGLLFARRYFRHMKTTSKPIYSADLFLIRTFRLGLAGNLF
SRLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLLALPDGNSS
LWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRM
TLLSIGGITLASSLVFNRLHVSDGTNLTRNTPS

Sequences:

>Translated_513_residues
MFFDNNYHYNAIPVRLLVNIQDADSDGIQTASENNNGTRMGYNRPMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNT
ALPEIATDLDESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSLFELTLSRVVQGIG
GSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGYLVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGN
DTALDFKGYLTFSAAACLLLLAAESLSHALPPYFALLPLCGGLLFARRYFRHMKTTSKPIYSADLFLIRTFRLGLAGNLF
SRLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLLALPDGNSS
LWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRM
TLLSIGGITLASSLVFNRLHVSDGTNLTRNTPS
>Mature_513_residues
MFFDNNYHYNAIPVRLLVNIQDADSDGIQTASENNNGTRMGYNRPMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNT
ALPEIATDLDESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSLFELTLSRVVQGIG
GSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGYLVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGN
DTALDFKGYLTFSAAACLLLLAAESLSHALPPYFALLPLCGGLLFARRYFRHMKTTSKPIYSADLFLIRTFRLGLAGNLF
SRLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLLALPDGNSS
LWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRM
TLLSIGGITLASSLVFNRLHVSDGTNLTRNTPS

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=452, Percent_Identity=43.141592920354, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1788392, Length=444, Percent_Identity=37.8378378378378, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1789042, Length=446, Percent_Identity=26.457399103139, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1788710, Length=337, Percent_Identity=29.9703264094956, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=26.3157894736842, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325455, Length=133, Percent_Identity=36.0902255639098, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55655; Mature: 55655

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFDNNYHYNAIPVRLLVNIQDADSDGIQTASENNNGTRMGYNRPMEKNTLSARAPSPWL
CCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
PLLLAIAIFMQMLDATILNTALPEIATDLDESPLDMQLAVVAYTLTVALLIPLSGYLADR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FGTKKVFFGSIAVFMLGSALCAASGSLFELTLSRVVQGIGGSMLVPIPRLTILRMYDKSK
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHH
LLNAINYAVMPALIGPVLGPLAGGYLVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGN
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCC
DTALDFKGYLTFSAAACLLLLAAESLSHALPPYFALLPLCGGLLFARRYFRHMKTTSKPI
CCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFS
HHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCEEH
AMNTLTLADLRPQQTGSGNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRM
HHCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
TLLSIGGITLASSLVFNRLHVSDGTNLTRNTPS
HHHHHCHHHHHHHHHHHHEECCCCCCCCCCCCC
>Mature Secondary Structure
MFFDNNYHYNAIPVRLLVNIQDADSDGIQTASENNNGTRMGYNRPMEKNTLSARAPSPWL
CCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
PLLLAIAIFMQMLDATILNTALPEIATDLDESPLDMQLAVVAYTLTVALLIPLSGYLADR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FGTKKVFFGSIAVFMLGSALCAASGSLFELTLSRVVQGIGGSMLVPIPRLTILRMYDKSK
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHH
LLNAINYAVMPALIGPVLGPLAGGYLVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGN
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCC
DTALDFKGYLTFSAAACLLLLAAESLSHALPPYFALLPLCGGLLFARRYFRHMKTTSKPI
CCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFS
HHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCEEH
AMNTLTLADLRPQQTGSGNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRM
HHCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
TLLSIGGITLASSLVFNRLHVSDGTNLTRNTPS
HHHHHCHHHHHHHHHHHHEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]