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The map label for this gene is greA [H]

Identifier: 161870299

GI number: 161870299

Start: 1354088

End: 1354609

Strand: Reverse

Name: greA [H]

Synonym: NMCC_1341

Alternate gene names: 161870299

Gene position: 1354609-1354088 (Counterclockwise)

Preceding gene: 161870301

Following gene: 161870296

Centisome position: 62.91

GC content: 53.07

Gene sequence:

>522_bases
TTGCTGCCGCAAACCGGTGTGCCGGAATATGGAGAAAAAAGACCGATGCAAAAAATCCCCCTGACCGTACGCGGTGCGGA
ATTGCTGAAACAGGAATTGCAGCAGCTTAAAAGCGTGGCGCGCCCCGAAGTGATCGAAGCGATTGCTGAAGCCAGATCGC
ACGGCGATTTGTCCGAAAACGCCGAATATGAAGCCGCCAAAGAACGCCAAGGCTTTATCGAAGGCCGCATTTCCGAGCTG
GAACACAAACTTTCCGTTGCCCACATCATCAATCCGACCGAAATCCACGCCGAAGGCAAAATCGTGTTCGGTACGACGGT
TACGCTGGAAGATTTGGAAACGGAAGAACACGTTACCTATCAAATTGTCGGCGAAGACGAAGCCGACATCAAACAGGGCA
AAATCTATGTCGGCTCACCGATTGCCCGCGCCTTAATCGGCAAGGAAGAGGGCGATACGGCGGAAGTCCAAGCCCCGGGC
GGCGTGCGCGAATACGACATTATCGAAGTCCGATATATTTGA

Upstream 100 bases:

>100_bases
ATAGCCGCTGTTCAACGACAGACAAGCCGCCGATTTTCCGGGCGGCTTGTGTTTTTATTTTAATATCCGGACGCACAAAA
CAAGACGGTATGCGGTATGT

Downstream 100 bases:

>100_bases
TTCGGCTTGATTTCGATACACCCGACACACGCAGGAAATTATAGTGGATTAACAAAAATCAGGACAAGGCGACGAAGCCG
CAGACAGTACAGATAGTACG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MLPQTGVPEYGEKRPMQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSENAEYEAAKERQGFIEGRISEL
EHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEHVTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPG
GVREYDIIEVRYI

Sequences:

>Translated_173_residues
MLPQTGVPEYGEKRPMQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSENAEYEAAKERQGFIEGRISEL
EHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEHVTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPG
GVREYDIIEVRYI
>Mature_173_residues
MLPQTGVPEYGEKRPMQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSENAEYEAAKERQGFIEGRISEL
EHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEHVTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPG
GVREYDIIEVRYI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=58.2278481012658, Blast_Score=189, Evalue=7e-50,
Organism=Escherichia coli, GI87082261, Length=157, Percent_Identity=38.8535031847134, Blast_Score=93, Evalue=9e-21,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 19187; Mature: 19187

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPQTGVPEYGEKRPMQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSEN
CCCCCCCCCCCCCCCHHHCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
AEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEHVTY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCEEEEEEEEEEECCCCCCCEEE
QIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI
EEECCCCCCCCCCEEEECCHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEC
>Mature Secondary Structure
MLPQTGVPEYGEKRPMQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSEN
CCCCCCCCCCCCCCCHHHCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
AEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEHVTY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCEEEEEEEEEEECCCCCCCEEE
QIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI
EEECCCCCCCCCCEEEECCHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]