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The map label for this gene is miaA [H]

Identifier: 161869846

GI number: 161869846

Start: 879330

End: 880142

Strand: Reverse

Name: miaA [H]

Synonym: NMCC_0877

Alternate gene names: 161869846

Gene position: 880142-879330 (Counterclockwise)

Preceding gene: 161869850

Following gene: 161869845

Centisome position: 40.87

GC content: 56.95

Gene sequence:

>813_bases
ATGGACATCGGCACGGCAAAACCGTCCGCCTCCGAACGCGCCTTTGTCCCGCACCACCTTATCGACATCATTACGCCTGT
TCAGACATACAGTGCCGCCCGTTTTGTCGAAGACTGCACGCGTCTGATCGGCGAAATCACGGCGCGTGGGAAATGTCCGC
TGATTGTCGGCGGCACAATGATGTATTTCCGCGCCCTGACCCAAGGTTTGAACGATTTGCCCGAGGCCGATGCCTGCCTG
CGTGCCGACTTGGACGAACAAAAACAGATGTACGGCTTGGATTTCCTCTACCGCACCCTGCAAAAAGTCGACCCTGAAAC
CGCATGCCGTCTGAAACCGAACGACAGCCAGCGTATCGAACGCGCTTTGGAAGTTTATTATCTGACCGGCAAACCGATGA
GCGAACACCTCGGCAGACAAAGCCCCCATACGCTCCCCTTCGATTTGCATACCGCCGCCCTGATTCCCGAAAACCGCGCC
CGCCTGCATGAAAACATCGCCCTGCGTTTCCACCTGATGCTTGAACAGGGCTTTATCGGCGAAGTAGAAAACCTGCGCCG
CCGCTATCCCGGCCTGACCGCCGACTCCCCCGCCATCCGCTGCGTCGGCTACCGTCAGGCATGGGAATATCTAGACGGAA
AAACCGATTTCCCGGCATTTGTCGAGAAAGGCATTGCCGCCACCCGCCAACTTGCCAAACGCCAACTGACTTGGTTGCGC
AAAACACCTTTAGACTGCGTTGCCGACCCGTTTTCAGACGGCACTTCATGCACACGCCTGATTGAGGCGGCAAAACGGTT
TTTCGGCGCATAA

Upstream 100 bases:

>100_bases
AGGCCCGACCGCCTGCGGCAAAACCGCGCTCGCCCTCAAAATTGCCGAAACCCTGCCTGTCGAAATCATCAGCCTCGATT
CCGCGCTAGTCTATACCGGT

Downstream 100 bases:

>100_bases
ACCATGCTGACCACACCGCCGCTCGCCCCCAAAACCGTCGCCGCCCTGCACAGGCTCGGTATCCGTACGCTCGAAGAACT
GCGGCAAAACGGTTCGGTCA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MDIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKCPLIVGGTMMYFRALTQGLNDLPEADACL
RADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQRIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRA
RLHENIALRFHLMLEQGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAKRQLTWLR
KTPLDCVADPFSDGTSCTRLIEAAKRFFGA

Sequences:

>Translated_270_residues
MDIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKCPLIVGGTMMYFRALTQGLNDLPEADACL
RADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQRIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRA
RLHENIALRFHLMLEQGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAKRQLTWLR
KTPLDCVADPFSDGTSCTRLIEAAKRFFGA
>Mature_270_residues
MDIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKCPLIVGGTMMYFRALTQGLNDLPEADACL
RADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQRIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRA
RLHENIALRFHLMLEQGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAKRQLTWLR
KTPLDCVADPFSDGTSCTRLIEAAKRFFGA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=271, Percent_Identity=30.2583025830258, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1790613, Length=240, Percent_Identity=50.8333333333333, Blast_Score=240, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI25144712, Length=235, Percent_Identity=25.531914893617, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324848, Length=250, Percent_Identity=32.4, Blast_Score=119, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24649830, Length=233, Percent_Identity=25.3218884120172, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 30502; Mature: 30502

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKCPLIVGGTM
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHH
MYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQRIE
HHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHH
RALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLEQGFIG
HHHHHHEECCCCHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCH
EVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAKRQLTWLR
HHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KTPLDCVADPFSDGTSCTRLIEAAKRFFGA
CCCHHHHCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKCPLIVGGTM
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHH
MYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQRIE
HHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHH
RALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLEQGFIG
HHHHHHEECCCCHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCH
EVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAKRQLTWLR
HHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KTPLDCVADPFSDGTSCTRLIEAAKRFFGA
CCCHHHHCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]