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The map label for this gene is rlmB [H]

Identifier: 161869843

GI number: 161869843

Start: 877296

End: 878000

Strand: Reverse

Name: rlmB [H]

Synonym: NMCC_0874

Alternate gene names: 161869843

Gene position: 878000-877296 (Counterclockwise)

Preceding gene: 161869844

Following gene: 161869841

Centisome position: 40.77

GC content: 55.32

Gene sequence:

>705_bases
TTGTGGCAAAACCCCAAATCCATCGTCGAACTCTACATCCAAGAAGGCAAATCAGACGCACGCACGCGCGAAGTGTTGGA
AAAGGCGGCAAACGAAAACATCCGCGTATATTTTGTTGACACCACCCGTCTTGATGCCATCAGCAAAGGCGCGCGCCATC
AGGGTGTGGCCGGATTTATCGATGCCTCCAAAAACCACGTCCATCTCGAAGACGTATTGGAAAACTTAAGCGAACCGCCG
CTGCTGCTGATACTCGACGGCATCACCGACCCGCACAACCTCGGCGCATGCCTGCGTACCGCCGACGCAATGGGCGTACA
CGCCGTCATCGCACCGAAAGACAAAAGCGCGGGGCTGAACGCCACCGTCAGCAAAGTCGCCTGCGGTGCGGCGGAAACCG
TCCCCTATATCACCGTAACCAACCTCGCCCGCACCCTGCGCGAATTGAAAGAATACGGCATTTGGATTATCGGCACCGAT
ATGAGCGGCGAGTCCGACCTTTACCATTGCAATCTGCCCGACAGAGCGGCGTGGGTAATGGGCAACGAAGGCGACGGTAT
GCGCCGCCTCACGCGCGAACATTGCGACATGCTGGTGTCTATACCCATGTTCGGCACAGTCGAAAGCATGAACGTCTCCG
TGTCTGCCGGAATGGTATTAAGTGAAACACGCCGACAACGCGTATTAAAAAACGAAAAAGTATAA

Upstream 100 bases:

>100_bases
CATACGGATAAATTGCTATAATGCCGTCTGAAATTTCCGACAAAGCACCATCATGGCAAACCAAAGACCCATCTACGGCT
TCCACGCCGTCAATGCCCGT

Downstream 100 bases:

>100_bases
GCCTCAAAAACAATGCCGTCTGAAACAGGGATTCTGTTTCAGACGGCATTCATTTTCAAAATCAGTGTTCCAAGTGGTCG
GTATCAAACTTGACCGCCGC

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase rlmB

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MWQNPKSIVELYIQEGKSDARTREVLEKAANENIRVYFVDTTRLDAISKGARHQGVAGFIDASKNHVHLEDVLENLSEPP
LLLILDGITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLARTLRELKEYGIWIIGTD
MSGESDLYHCNLPDRAAWVMGNEGDGMRRLTREHCDMLVSIPMFGTVESMNVSVSAGMVLSETRRQRVLKNEKV

Sequences:

>Translated_234_residues
MWQNPKSIVELYIQEGKSDARTREVLEKAANENIRVYFVDTTRLDAISKGARHQGVAGFIDASKNHVHLEDVLENLSEPP
LLLILDGITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLARTLRELKEYGIWIIGTD
MSGESDLYHCNLPDRAAWVMGNEGDGMRRLTREHCDMLVSIPMFGTVESMNVSVSAGMVLSETRRQRVLKNEKV
>Mature_234_residues
MWQNPKSIVELYIQEGKSDARTREVLEKAANENIRVYFVDTTRLDAISKGARHQGVAGFIDASKNHVHLEDVLENLSEPP
LLLILDGITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLARTLRELKEYGIWIIGTD
MSGESDLYHCNLPDRAAWVMGNEGDGMRRLTREHCDMLVSIPMFGTVESMNVSVSAGMVLSETRRQRVLKNEKV

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=241, Percent_Identity=30.7053941908714, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI1790623, Length=225, Percent_Identity=52, Blast_Score=234, Evalue=3e-63,
Organism=Escherichia coli, GI1788935, Length=222, Percent_Identity=27.027027027027, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790083, Length=153, Percent_Identity=32.0261437908497, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17555908, Length=226, Percent_Identity=26.5486725663717, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324775, Length=167, Percent_Identity=31.7365269461078, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25745; Mature: 25745

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWQNPKSIVELYIQEGKSDARTREVLEKAANENIRVYFVDTTRLDAISKGARHQGVAGFI
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHCCCCCCCCEEEE
DASKNHVHLEDVLENLSEPPLLLILDGITDPHNLGACLRTADAMGVHAVIAPKDKSAGLN
ECCCCEEEHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCC
ATVSKVACGAAETVPYITVTNLARTLRELKEYGIWIIGTDMSGESDLYHCNLPDRAAWVM
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCEEEEE
GNEGDGMRRLTREHCDMLVSIPMFGTVESMNVSVSAGMVLSETRRQRVLKNEKV
CCCCCHHHHHHHHHHHEEEECCCCCCEECCEEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MWQNPKSIVELYIQEGKSDARTREVLEKAANENIRVYFVDTTRLDAISKGARHQGVAGFI
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHCCCCCCCCEEEE
DASKNHVHLEDVLENLSEPPLLLILDGITDPHNLGACLRTADAMGVHAVIAPKDKSAGLN
ECCCCEEEHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCC
ATVSKVACGAAETVPYITVTNLARTLRELKEYGIWIIGTDMSGESDLYHCNLPDRAAWVM
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCEEEEE
GNEGDGMRRLTREHCDMLVSIPMFGTVESMNVSVSAGMVLSETRRQRVLKNEKV
CCCCCHHHHHHHHHHHEEEECCCCCCEECCEEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]