Click here to switch to the map view.

The map label for this gene is ispG [H]

Identifier: 161524636

GI number: 161524636

Start: 1608679

End: 1609995

Strand: Direct

Name: ispG [H]

Synonym: Bmul_1463

Alternate gene names: 161524636

Gene position: 1608679-1609995 (Clockwise)

Preceding gene: 161524635

Following gene: 161524637

Centisome position: 46.65

GC content: 67.5

Gene sequence:

>1317_bases
ATGGATCTATCGATGCAATCCGAAGCTCAATCCCCACGCAGCAGTCAGATTTGTTCAACCGAACCGGTATTCGGCGGGCC
GGCGCCGCGTCGCGTATCGCATGCGGTCGATGTCCGTTGGGGCGGGCAGCTCGTGACGATCGGCGGCGACGCGCCGGTGC
GCGTGCAGTCGATGACGAACACCGACACGGCCGACGCGATCGGCACCGCGATCCAGATCAAGGAACTCGCGAACGCGGGC
TCCGAGCTGGTGCGCATCACCGTCAACACGCCGGAGGCCGCGGCCGCCGTGCCGGCGATCCGCGAGCAGCTCGACCGGAT
GGGCGTGACGGTGCCGCTCGTCGGCGATTTCCATTACAACGGCCACCTGCTGCTGCGCGACTACCCGGGCTGCGCGGAAG
CGCTCTCGAAGTACCGGATCAATCCGGGCAACGTCGGTCAGGGCGCGAAGCGCGACACGCAGTTCGCGCAGATGATCGAA
GCGGCGATCAAGTACGACAAGCCGGTGCGGATCGGCGTGAACTGGGGCAGCCTCGATCAGGATCTGCTCGCGCGGATGAT
GGACGAGAATGCCGCGCGCGCGCAGCCGTGGGATGCGCAGAGCGTCATGTACGAGGCGCTGATCCAGTCGGCGATCGGCT
CGGCCGAGCGTGCGGTCGAACTGGGCCTCGGGCGCGACCGCATCGTGCTGTCGTGCAAGGTCAGCGGCGTGCAGGACCTG
ATCGCGGTGTACCGCGAGCTCGCGCGCCGCTGCGGCTTCGCGCTGCACCTCGGCCTGACCGAGGCCGGCATGGGCTCGAA
GGGCATCGTCGCGTCGACGGCGGCGCTCGGCGTGCTGCTGCAGGAAGGGATCGGCGACACGATCCGCATTTCGCTGACAC
CGGAACCGGGCGCGCCGCGCACGGGCGAAGTGGTGGTCGGCCAGGAAATCCTGCAGACGATGGGCCTGCGCTCGTTCGCG
CCGATGGTGATCGCATGCCCGGGCTGCGGCCGCACGACGAGCACGCTGTTCCAGGAACTGGCGATGCAGATCCAGACGTA
TCTGCGCGAGCAGATGCCGGTGTGGCGCAAGGAATATCCGGGCGTCGAGAAGATGAACGTCGCGGTGATGGGCTGTATCG
TCAACGGCCCGGGCGAGTCGAAGCACGCGAACATCGGCATCAGCCTGCCGGGCTCGGGCGAGAACCCGGCCGCGCCCGTC
TTCATCGACGGCGAGAAGGTCAAGACGCTGCGCGGCGAGCGGATCGCCGAGGAATTCCAGCAGATCGTCAGCGACTATGT
CGCACGCAATTACGGCCGCGCGGCGGCCACGAACTAA

Upstream 100 bases:

>100_bases
GATGCCAAGCGCCGTGGCCGGAGGCTGCCGCCGCGTCGGCAGCCGTGGCAGCACGGCGCTTTTTCAATTCGTGCGCCGCC
TCGCGGCGCATTCCGCGTAA

Downstream 100 bases:

>100_bases
TTCGTCCACACACAGATGACTGAACAGAAACGCAAGATCGAGAAGCTCACGGGCGTCAAGGGCATGAACGACATCCTGCC
GCAGGATGCCGGTCTGTGGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MDLSMQSEAQSPRSSQICSTEPVFGGPAPRRVSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELANAG
SELVRITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPGCAEALSKYRINPGNVGQGAKRDTQFAQMIE
AAIKYDKPVRIGVNWGSLDQDLLARMMDENAARAQPWDAQSVMYEALIQSAIGSAERAVELGLGRDRIVLSCKVSGVQDL
IAVYRELARRCGFALHLGLTEAGMGSKGIVASTAALGVLLQEGIGDTIRISLTPEPGAPRTGEVVVGQEILQTMGLRSFA
PMVIACPGCGRTTSTLFQELAMQIQTYLREQMPVWRKEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPV
FIDGEKVKTLRGERIAEEFQQIVSDYVARNYGRAAATN

Sequences:

>Translated_438_residues
MDLSMQSEAQSPRSSQICSTEPVFGGPAPRRVSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELANAG
SELVRITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPGCAEALSKYRINPGNVGQGAKRDTQFAQMIE
AAIKYDKPVRIGVNWGSLDQDLLARMMDENAARAQPWDAQSVMYEALIQSAIGSAERAVELGLGRDRIVLSCKVSGVQDL
IAVYRELARRCGFALHLGLTEAGMGSKGIVASTAALGVLLQEGIGDTIRISLTPEPGAPRTGEVVVGQEILQTMGLRSFA
PMVIACPGCGRTTSTLFQELAMQIQTYLREQMPVWRKEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPV
FIDGEKVKTLRGERIAEEFQQIVSDYVARNYGRAAATN
>Mature_438_residues
MDLSMQSEAQSPRSSQICSTEPVFGGPAPRRVSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELANAG
SELVRITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPGCAEALSKYRINPGNVGQGAKRDTQFAQMIE
AAIKYDKPVRIGVNWGSLDQDLLARMMDENAARAQPWDAQSVMYEALIQSAIGSAERAVELGLGRDRIVLSCKVSGVQDL
IAVYRELARRCGFALHLGLTEAGMGSKGIVASTAALGVLLQEGIGDTIRISLTPEPGAPRTGEVVVGQEILQTMGLRSFA
PMVIACPGCGRTTSTLFQELAMQIQTYLREQMPVWRKEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPV
FIDGEKVKTLRGERIAEEFQQIVSDYVARNYGRAAATN

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=376, Percent_Identity=34.8404255319149, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 46902; Mature: 46902

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSMQSEAQSPRSSQICSTEPVFGGPAPRRVSHAVDVRWGGQLVTIGGDAPVRVQSMTN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEECCEEEEECCCCCEEEEECCC
TDTADAIGTAIQIKELANAGSELVRITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYN
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEC
GHLLLRDYPGCAEALSKYRINPGNVGQGAKRDTQFAQMIEAAIKYDKPVRIGVNWGSLDQ
CEEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCH
DLLARMMDENAARAQPWDAQSVMYEALIQSAIGSAERAVELGLGRDRIVLSCKVSGVQDL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHH
IAVYRELARRCGFALHLGLTEAGMGSKGIVASTAALGVLLQEGIGDTIRISLTPEPGAPR
HHHHHHHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
TGEVVVGQEILQTMGLRSFAPMVIACPGCGRTTSTLFQELAMQIQTYLREQMPVWRKEYP
CCCEEECHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
GVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFIDGEKVKTLRGERIAEEFQ
CCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
QIVSDYVARNYGRAAATN
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDLSMQSEAQSPRSSQICSTEPVFGGPAPRRVSHAVDVRWGGQLVTIGGDAPVRVQSMTN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEECCEEEEECCCCCEEEEECCC
TDTADAIGTAIQIKELANAGSELVRITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYN
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEC
GHLLLRDYPGCAEALSKYRINPGNVGQGAKRDTQFAQMIEAAIKYDKPVRIGVNWGSLDQ
CEEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCH
DLLARMMDENAARAQPWDAQSVMYEALIQSAIGSAERAVELGLGRDRIVLSCKVSGVQDL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHH
IAVYRELARRCGFALHLGLTEAGMGSKGIVASTAALGVLLQEGIGDTIRISLTPEPGAPR
HHHHHHHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
TGEVVVGQEILQTMGLRSFAPMVIACPGCGRTTSTLFQELAMQIQTYLREQMPVWRKEYP
CCCEEECHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
GVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFIDGEKVKTLRGERIAEEFQ
CCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
QIVSDYVARNYGRAAATN
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA