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The map label for this gene is hisS [H]

Identifier: 161524637

GI number: 161524637

Start: 1610011

End: 1611351

Strand: Direct

Name: hisS [H]

Synonym: Bmul_1464

Alternate gene names: 161524637

Gene position: 1610011-1611351 (Clockwise)

Preceding gene: 161524636

Following gene: 161524638

Centisome position: 46.69

GC content: 64.13

Gene sequence:

>1341_bases
ATGACTGAACAGAAACGCAAGATCGAGAAGCTCACGGGCGTCAAGGGCATGAACGACATCCTGCCGCAGGATGCCGGTCT
GTGGGAGTTCTTCGAGTCCACCGTGAAATCGCTGTTGCGCGCGTACGGCTATCAGAACATCCGCACGCCGATCGTCGAGC
ATACGCAGCTCTTCACGCGCGGCATCGGCGAAGTCACCGACATCGTCGAGAAGGAGATGTACAGCTTCACCGACGCGCTG
AACGGCGAGCACCTGACGATGCGTCCGGAGAACACCGCGGCGGTCGTGCGCGCGGCGATCGAGCACAACATGCTGTACGA
CGGCCCGAAGCGCCTCTGGTACATCGGCCCGATGTTCCGTCACGAGCGTCCGCAGCGCGGCCGCTATCGGCAGTTCCATC
AGGTCGGCGTCGAGGCGCTCGGCTTCGCGGGTCCCGATGCGGATGCCGAGATCATCATGATGTGCCAGCGTCTGTGGGAC
GACCTCGGGCTGACCGGCATCAAGCTCGAGATCAACTCGCTCGGCCTCGCCGAAGAGCGTGCAGCGCATCGCGTCGAACT
GATCAAGTATCTCGAGCAGCACGTCGACGCGCTCGACGAAGATGCGAAGCGTCGTCTGTATACGAACCCGCTGCGCGTGC
TCGACACGAAGAATCCGGCGCTGCAGGAAATCGCGCAGAACGCGCCGAAGCTGATCGACTTCCTCGGCGACACGTCGCGT
GCGCACTTCGAGGGGCTGCAGCGCCTGCTGCTCGCGAACAACATTCCGTTCAAGATCAATCCGCGGCTCGTGCGCGGCCT
CGACTACTACAACCTCACCGTGTTCGAGTGGGTCACCGACAAGCTCGGCGCGCAGGGTACCGTTGCGGCCGGCGGCCGTT
ACGATCCGCTGATCGAGCAGCTCGGCGGCAAGCCGACGGCTGCGTGCGGCTGGGCGATGGGCATCGAGCGCATTCTCGAG
CTGCTGAAGGAAGAGAACCTTGCGCCGGAGCAGGAAGGCGTCGACGTGTACGTCGTGCACCAGGGCGACGCGGCGCGCGA
GCAGGCCTTCATCGCGGCCGAGCGTCTGCGCGATACGGGCCTCGACGTGATCTTCCACTGCAGCGCCGATGGCGCGCCGG
CAAGCTTCAAGTCGCAGATGAAGCGCGCCGATGCGAGCGGCGCCGCATTCGCGGTGATCTTCGGCGAAGAAGAAGTCGCG
AACGGCACGGTGGGCGTGAAAGCGCTGCGCGGCACGGGCGGCGACGGGGAAAAGAACGCTCAGCAGACCGTACCGGTCGA
AAGCTTGACCGAATTCCTAATCAATGCGATGGTTGCATCCGCCGAAGACGGCGACGACTGA

Upstream 100 bases:

>100_bases
CGGCGAGCGGATCGCCGAGGAATTCCAGCAGATCGTCAGCGACTATGTCGCACGCAATTACGGCCGCGCGGCGGCCACGA
ACTAATTCGTCCACACACAG

Downstream 100 bases:

>100_bases
TCGCGCTGGCGTTCGCTCGACAAGAAAAGGCCTGAACAGGAAGGAATCGCTCGCCGATGAGTTATCACGACGAACAAGAA
TCGATTGAAAGTCTGAAAGC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTEQKRKIEKLTGVKGMNDILPQDAGLWEFFESTVKSLLRAYGYQNIRTPIVEHTQLFTRGIGEVTDIVEKEMYSFTDAL
NGEHLTMRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFRHERPQRGRYRQFHQVGVEALGFAGPDADAEIIMMCQRLWD
DLGLTGIKLEINSLGLAEERAAHRVELIKYLEQHVDALDEDAKRRLYTNPLRVLDTKNPALQEIAQNAPKLIDFLGDTSR
AHFEGLQRLLLANNIPFKINPRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQLGGKPTAACGWAMGIERILE
LLKEENLAPEQEGVDVYVVHQGDAAREQAFIAAERLRDTGLDVIFHCSADGAPASFKSQMKRADASGAAFAVIFGEEEVA
NGTVGVKALRGTGGDGEKNAQQTVPVESLTEFLINAMVASAEDGDD

Sequences:

>Translated_446_residues
MTEQKRKIEKLTGVKGMNDILPQDAGLWEFFESTVKSLLRAYGYQNIRTPIVEHTQLFTRGIGEVTDIVEKEMYSFTDAL
NGEHLTMRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFRHERPQRGRYRQFHQVGVEALGFAGPDADAEIIMMCQRLWD
DLGLTGIKLEINSLGLAEERAAHRVELIKYLEQHVDALDEDAKRRLYTNPLRVLDTKNPALQEIAQNAPKLIDFLGDTSR
AHFEGLQRLLLANNIPFKINPRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQLGGKPTAACGWAMGIERILE
LLKEENLAPEQEGVDVYVVHQGDAAREQAFIAAERLRDTGLDVIFHCSADGAPASFKSQMKRADASGAAFAVIFGEEEVA
NGTVGVKALRGTGGDGEKNAQQTVPVESLTEFLINAMVASAEDGDD
>Mature_445_residues
TEQKRKIEKLTGVKGMNDILPQDAGLWEFFESTVKSLLRAYGYQNIRTPIVEHTQLFTRGIGEVTDIVEKEMYSFTDALN
GEHLTMRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFRHERPQRGRYRQFHQVGVEALGFAGPDADAEIIMMCQRLWDD
LGLTGIKLEINSLGLAEERAAHRVELIKYLEQHVDALDEDAKRRLYTNPLRVLDTKNPALQEIAQNAPKLIDFLGDTSRA
HFEGLQRLLLANNIPFKINPRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQLGGKPTAACGWAMGIERILEL
LKEENLAPEQEGVDVYVVHQGDAAREQAFIAAERLRDTGLDVIFHCSADGAPASFKSQMKRADASGAAFAVIFGEEEVAN
GTVGVKALRGTGGDGEKNAQQTVPVESLTEFLINAMVASAEDGDD

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=420, Percent_Identity=54.5238095238095, Blast_Score=447, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 49528; Mature: 49397

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQKRKIEKLTGVKGMNDILPQDAGLWEFFESTVKSLLRAYGYQNIRTPIVEHTQLFTR
CCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GIGEVTDIVEKEMYSFTDALNGEHLTMRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFR
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHH
HERPQRGRYRQFHQVGVEALGFAGPDADAEIIMMCQRLWDDLGLTGIKLEINSLGLAEER
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHH
AAHRVELIKYLEQHVDALDEDAKRRLYTNPLRVLDTKNPALQEIAQNAPKLIDFLGDTSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHCCHHHHHHHCCCCH
AHFEGLQRLLLANNIPFKINPRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQ
HHHHHHHHHHHHCCCCEEECHHHHHCCCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHH
LGGKPTAACGWAMGIERILELLKEENLAPEQEGVDVYVVHQGDAAREQAFIAAERLRDTG
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
LDVIFHCSADGAPASFKSQMKRADASGAAFAVIFGEEEVANGTVGVKALRGTGGDGEKNA
CCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCCEEEEECCCCCCCCCC
QQTVPVESLTEFLINAMVASAEDGDD
HHCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEQKRKIEKLTGVKGMNDILPQDAGLWEFFESTVKSLLRAYGYQNIRTPIVEHTQLFTR
CCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GIGEVTDIVEKEMYSFTDALNGEHLTMRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFR
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHH
HERPQRGRYRQFHQVGVEALGFAGPDADAEIIMMCQRLWDDLGLTGIKLEINSLGLAEER
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHH
AAHRVELIKYLEQHVDALDEDAKRRLYTNPLRVLDTKNPALQEIAQNAPKLIDFLGDTSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHCCHHHHHHHCCCCH
AHFEGLQRLLLANNIPFKINPRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQ
HHHHHHHHHHHHCCCCEEECHHHHHCCCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHH
LGGKPTAACGWAMGIERILELLKEENLAPEQEGVDVYVVHQGDAAREQAFIAAERLRDTG
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
LDVIFHCSADGAPASFKSQMKRADASGAAFAVIFGEEEVANGTVGVKALRGTGGDGEKNA
CCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCCEEEEECCCCCCCCCC
QQTVPVESLTEFLINAMVASAEDGDD
HHCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA