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The map label for this gene is walK [H]

Identifier: 160902341

GI number: 160902341

Start: 930394

End: 931674

Strand: Direct

Name: walK [H]

Synonym: Pmob_0876

Alternate gene names: 160902341

Gene position: 930394-931674 (Clockwise)

Preceding gene: 160902340

Following gene: 160902342

Centisome position: 42.88

GC content: 28.34

Gene sequence:

>1281_bases
TTGGTCAATATATATATTATCGTTATTTTATTGCTTATTATCACAAATATTTTATTTATATATGCTTATTCAAAAAAGAG
AAAAGAAGAAAAGGCTCATCAACGGTTTAAAAATGAGATTGCAAAAAGCATAAATATAAATATAAAAAATCCTGTTGATG
ATTTTCTTTTGCATCAACTAACTTTGTATATTAAAAATTTAGAAGAAAAATACAAATCCGAAAAATACAAAAGAAGGAAC
ATATTTTCGATTCTAGATACCCTTTCCGAAGGAATAATATTGGTTTCTTATAATCAAAATGAAATTATCAGGGTGGATTT
CGCTAATTCTTTTGCCAAAAATATTTTCACAACAGAAAATTTTGTCGGTAGGTCATTAACAGAGGTAGTTGACAATCACA
ATCTCATTGAATTGACTTTAAAAAGTTTTAAAACTAATAAAGATTTGGAAGAAGAAACTTCATTCTATTATCCAGAAAAA
AAATACTTCAGATGTCAAGTTAAATCAATAAATGTTGAAAATTATAGAGTTATTATTTTGTTAGATATCACGAAAGAAAA
AAATTTAGAAGATCTAAGAAGGGAATTCTTAACCATCATGTCGCATGAAATGCGGACACCTTTATCTGTTATAAGTGGAT
ACTTAGAAACGATATTGCACGAACCTAATTTAGACCTAGAAATTTATCAACCACTAAAAAAAATAGAAGAAGAAATATCG
AGATTAACCAGAATGTTTAACGATCTTTTAGATATAGAGAGATTGGAAAAAAATATAGGCGAAGAAAAAAGATTTGTATA
TTTCAATTTTTCTAATACGGTAAAGAGAGCCTTTGATTTCTTTAAAATTGTTGCTGATAAGATGTCCATTGAGTTTGAAG
AACAAATAGAAGATGATCTATACGTTTTCGGTAATGAAGATAGACTCCTTCAGGTAGTGTATAATATTTTAGACAATTCT
TTTAAATTCACAGCTTTAAAGGAAAAAGGCGAAAAGAAAGTATGGTTAAGATTGTACGAAAATGACAATACTATAATTTT
GGAAATTGAAGATACAGGAATAGGCATACCTTCAAAAGAATTAAAAAGGATTTTCGATCTATTTTACAGAGTAGACAAAT
CGAGAAGCAGGCAAGTACCAGGCTTGGGGATTGGTTTGTACATAGTCAAAACTGTTTTGGATAACCACAATGCAAGAATA
TATTTAGACAGTGAAGAAAACAACGGTACATTGTTTCAAGTCATCCTTCCTATCAAAACAAATAATGAAACGGAGGTTTA
A

Upstream 100 bases:

>100_bases
AGATCCTGGAAAGGAAAGAAACTACTAAATATTCAATGGCAGAGGGTAATACCTCTGCCTTTTTTCCAATTGGATTTTAT
TAATAAACTAGGTGAAAATA

Downstream 100 bases:

>100_bases
AGTTGAGAGCATACGATATTATAAAAAATAAAAGAGATGCTAAAGAAAATACAAGAGAAGAAATAGAATTTATGATCAAT
GGCTATTTAGAAAACCGTAT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MVNIYIIVILLLIITNILFIYAYSKKRKEEKAHQRFKNEIAKSININIKNPVDDFLLHQLTLYIKNLEEKYKSEKYKRRN
IFSILDTLSEGIILVSYNQNEIIRVDFANSFAKNIFTTENFVGRSLTEVVDNHNLIELTLKSFKTNKDLEEETSFYYPEK
KYFRCQVKSINVENYRVIILLDITKEKNLEDLRREFLTIMSHEMRTPLSVISGYLETILHEPNLDLEIYQPLKKIEEEIS
RLTRMFNDLLDIERLEKNIGEEKRFVYFNFSNTVKRAFDFFKIVADKMSIEFEEQIEDDLYVFGNEDRLLQVVYNILDNS
FKFTALKEKGEKKVWLRLYENDNTIILEIEDTGIGIPSKELKRIFDLFYRVDKSRSRQVPGLGIGLYIVKTVLDNHNARI
YLDSEENNGTLFQVILPIKTNNETEV

Sequences:

>Translated_426_residues
MVNIYIIVILLLIITNILFIYAYSKKRKEEKAHQRFKNEIAKSININIKNPVDDFLLHQLTLYIKNLEEKYKSEKYKRRN
IFSILDTLSEGIILVSYNQNEIIRVDFANSFAKNIFTTENFVGRSLTEVVDNHNLIELTLKSFKTNKDLEEETSFYYPEK
KYFRCQVKSINVENYRVIILLDITKEKNLEDLRREFLTIMSHEMRTPLSVISGYLETILHEPNLDLEIYQPLKKIEEEIS
RLTRMFNDLLDIERLEKNIGEEKRFVYFNFSNTVKRAFDFFKIVADKMSIEFEEQIEDDLYVFGNEDRLLQVVYNILDNS
FKFTALKEKGEKKVWLRLYENDNTIILEIEDTGIGIPSKELKRIFDLFYRVDKSRSRQVPGLGIGLYIVKTVLDNHNARI
YLDSEENNGTLFQVILPIKTNNETEV
>Mature_426_residues
MVNIYIIVILLLIITNILFIYAYSKKRKEEKAHQRFKNEIAKSININIKNPVDDFLLHQLTLYIKNLEEKYKSEKYKRRN
IFSILDTLSEGIILVSYNQNEIIRVDFANSFAKNIFTTENFVGRSLTEVVDNHNLIELTLKSFKTNKDLEEETSFYYPEK
KYFRCQVKSINVENYRVIILLDITKEKNLEDLRREFLTIMSHEMRTPLSVISGYLETILHEPNLDLEIYQPLKKIEEEIS
RLTRMFNDLLDIERLEKNIGEEKRFVYFNFSNTVKRAFDFFKIVADKMSIEFEEQIEDDLYVFGNEDRLLQVVYNILDNS
FKFTALKEKGEKKVWLRLYENDNTIILEIEDTGIGIPSKELKRIFDLFYRVDKSRSRQVPGLGIGLYIVKTVLDNHNARI
YLDSEENNGTLFQVILPIKTNNETEV

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=245, Percent_Identity=31.8367346938775, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788393, Length=252, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786783, Length=224, Percent_Identity=30.8035714285714, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI48994928, Length=295, Percent_Identity=28.135593220339, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1789149, Length=241, Percent_Identity=31.5352697095436, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI145693157, Length=264, Percent_Identity=28.7878787878788, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=26.0330578512397, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081816, Length=232, Percent_Identity=27.5862068965517, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788713, Length=270, Percent_Identity=27.037037037037, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=235, Percent_Identity=26.8085106382979, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=26.3829787234043, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789808, Length=416, Percent_Identity=22.1153846153846, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=23.1818181818182, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50527; Mature: 50527

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNIYIIVILLLIITNILFIYAYSKKRKEEKAHQRFKNEIAKSININIKNPVDDFLLHQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHH
TLYIKNLEEKYKSEKYKRRNIFSILDTLSEGIILVSYNQNEIIRVDFANSFAKNIFTTEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECHHHHHHHHCCCHH
FVGRSLTEVVDNHNLIELTLKSFKTNKDLEEETSFYYPEKKYFRCQVKSINVENYRVIIL
HHHHHHHHHHCCCCEEEEEHHHHCCCCCCHHHHCCCCCCCCEEEEEEEEECCCCEEEEEE
LDITKEKNLEDLRREFLTIMSHEMRTPLSVISGYLETILHEPNLDLEIYQPLKKIEEEIS
EEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RLTRMFNDLLDIERLEKNIGEEKRFVYFNFSNTVKRAFDFFKIVADKMSIEFEEQIEDDL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHCCCCE
YVFGNEDRLLQVVYNILDNSFKFTALKEKGEKKVWLRLYENDNTIILEIEDTGIGIPSKE
EEECCCHHHHHHHHHHHCCCEEEEEEHHCCCCEEEEEEEECCCEEEEEEECCCCCCCHHH
LKRIFDLFYRVDKSRSRQVPGLGIGLYIVKTVLDNHNARIYLDSEENNGTLFQVILPIKT
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEEC
NNETEV
CCCCCC
>Mature Secondary Structure
MVNIYIIVILLLIITNILFIYAYSKKRKEEKAHQRFKNEIAKSININIKNPVDDFLLHQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHH
TLYIKNLEEKYKSEKYKRRNIFSILDTLSEGIILVSYNQNEIIRVDFANSFAKNIFTTEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECHHHHHHHHCCCHH
FVGRSLTEVVDNHNLIELTLKSFKTNKDLEEETSFYYPEKKYFRCQVKSINVENYRVIIL
HHHHHHHHHHCCCCEEEEEHHHHCCCCCCHHHHCCCCCCCCEEEEEEEEECCCCEEEEEE
LDITKEKNLEDLRREFLTIMSHEMRTPLSVISGYLETILHEPNLDLEIYQPLKKIEEEIS
EEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RLTRMFNDLLDIERLEKNIGEEKRFVYFNFSNTVKRAFDFFKIVADKMSIEFEEQIEDDL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHCCCCE
YVFGNEDRLLQVVYNILDNSFKFTALKEKGEKKVWLRLYENDNTIILEIEDTGIGIPSKE
EEECCCHHHHHHHHHHHCCCEEEEEEHHCCCCEEEEEEEECCCEEEEEEECCCCCCCHHH
LKRIFDLFYRVDKSRSRQVPGLGIGLYIVKTVLDNHNARIYLDSEENNGTLFQVILPIKT
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEEC
NNETEV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA