Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 160901707

GI number: 160901707

Start: 221582

End: 222880

Strand: Direct

Name: purB [H]

Synonym: Pmob_0219

Alternate gene names: 160901707

Gene position: 221582-222880 (Clockwise)

Preceding gene: 160901706

Following gene: 160901708

Centisome position: 10.21

GC content: 34.72

Gene sequence:

>1299_bases
ATGTTGATTGAAAGGTACTCTCTTTCTCCAATTAAAGACATTTGGACTTTAGATGCCCAGTATAAAAGATGGCTTGAAGT
AGAAATAGCTGTAATAGAAGCCTTTGAAGAACTTAACCTTGCTCCCAAAGGCACATCCCAAAACATTCGACAAAAAGCAA
AATTAGACGTTCAAAAAATATTAGACACAGAAAAAATAGTGGATCATGACGTGATAGCCTTTATAAAAGTGGTAACTGAA
GACATGGGAGATGAGGCAAGATACTTTCACAAGGGTTTAACATCATCAGATGTTGTCGATACAGCTTTATCCTTAGCTAT
AAAAAGAGCAGAAGAGATTATTCTTGATGAGCTAACTCAATACATAGCTAACCTCAAAAAATTAGCTGTGGATCATAAGT
ATACTATTATAGTTGGAAGAACCCATGGGGTACACGCTGAGCCAACATCTTTTGGATTAAAAATTTTAGGATTTGTTGCG
GAAGAAGAAAGAAACAAAGAAAGATTAGAAAAGGCTATAGATAACATTTCCCAAGGAAAACTCAGCGGGGCGGTTGGAAA
TTATGCTAACATAGAACCAAAAGTAGAGGAAATCGCTTTGAAAAAACTCAATCTCAGACCTTGTAAAGTATCTACTCAAG
TTTTACCAAGGGATCTTCACGCTGAATTTTTCAGTGTTTTAGCTATGATAGGAAGCAGTATAGAAAGACTTGCCATAGAA
ATCAGGCACCTTCAAAAAACAGAGGTCCTGGAAGTAGAAGAACCTTTTAAAAAAGAGCAACGGGGCTCTTCAGCGATGCC
ACATAAGAAAAACCCCATATTGTGCGAAAGGTTGACCGGGATGTCAAGAATGCTAAGATCTTATCTATCTTCTGCTTATG
AGAATATCTCCTTATGGCACGAAAGAGATATTTCTCATTCGTCTGTAGAAAGGGTGTTTATACCGGATGCAACCATGCTT
ACTTACTATATGTTGAATAAAGCAAATTACTTAGTTGAAAACTTAGTGGTTCACAAAGATAGAATGAAAGAAAACATAGA
AAAATCGTATAATTTAATTTATTCTCAACGGGTATTGCTGAAGTTAGTTGAATTAGGGGTAAGTAGAGAGGAAGCTTATA
AAATAGTTCAAGAAAACGCTATGAAAGCTTGGAATGAAAGGCGTGATTTTAAAGCAATTTTATTGGAAGATAATAGAGTA
AAGGCAAAATTATCAGATAAAGAAGCATTTGAAGATATATTCTCACCTGATTATTATCTAAAAAATATAAATGAAATTTA
TGAAAGGTTTGAACTTTAG

Upstream 100 bases:

>100_bases
ATAGAAGTTTTAGAATCTCATGGTTTACGAGGTATCCTTATGGACGCAGTAAAAGAAGCAACTATAAGGTCAAAAGAACT
TAATTCACAAAGGGGTGTGG

Downstream 100 bases:

>100_bases
TAGAGGGCTGCAGGCCCATTCCTTAGAAGGGTGGGGAGCGGGGCGAAGGGGCCGCTATTAAACAAGTTTTTAGAATTTTT
AACAACGGTAAAAGTGCAAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MLIERYSLSPIKDIWTLDAQYKRWLEVEIAVIEAFEELNLAPKGTSQNIRQKAKLDVQKILDTEKIVDHDVIAFIKVVTE
DMGDEARYFHKGLTSSDVVDTALSLAIKRAEEIILDELTQYIANLKKLAVDHKYTIIVGRTHGVHAEPTSFGLKILGFVA
EEERNKERLEKAIDNISQGKLSGAVGNYANIEPKVEEIALKKLNLRPCKVSTQVLPRDLHAEFFSVLAMIGSSIERLAIE
IRHLQKTEVLEVEEPFKKEQRGSSAMPHKKNPILCERLTGMSRMLRSYLSSAYENISLWHERDISHSSVERVFIPDATML
TYYMLNKANYLVENLVVHKDRMKENIEKSYNLIYSQRVLLKLVELGVSREEAYKIVQENAMKAWNERRDFKAILLEDNRV
KAKLSDKEAFEDIFSPDYYLKNINEIYERFEL

Sequences:

>Translated_432_residues
MLIERYSLSPIKDIWTLDAQYKRWLEVEIAVIEAFEELNLAPKGTSQNIRQKAKLDVQKILDTEKIVDHDVIAFIKVVTE
DMGDEARYFHKGLTSSDVVDTALSLAIKRAEEIILDELTQYIANLKKLAVDHKYTIIVGRTHGVHAEPTSFGLKILGFVA
EEERNKERLEKAIDNISQGKLSGAVGNYANIEPKVEEIALKKLNLRPCKVSTQVLPRDLHAEFFSVLAMIGSSIERLAIE
IRHLQKTEVLEVEEPFKKEQRGSSAMPHKKNPILCERLTGMSRMLRSYLSSAYENISLWHERDISHSSVERVFIPDATML
TYYMLNKANYLVENLVVHKDRMKENIEKSYNLIYSQRVLLKLVELGVSREEAYKIVQENAMKAWNERRDFKAILLEDNRV
KAKLSDKEAFEDIFSPDYYLKNINEIYERFEL
>Mature_432_residues
MLIERYSLSPIKDIWTLDAQYKRWLEVEIAVIEAFEELNLAPKGTSQNIRQKAKLDVQKILDTEKIVDHDVIAFIKVVTE
DMGDEARYFHKGLTSSDVVDTALSLAIKRAEEIILDELTQYIANLKKLAVDHKYTIIVGRTHGVHAEPTSFGLKILGFVA
EEERNKERLEKAIDNISQGKLSGAVGNYANIEPKVEEIALKKLNLRPCKVSTQVLPRDLHAEFFSVLAMIGSSIERLAIE
IRHLQKTEVLEVEEPFKKEQRGSSAMPHKKNPILCERLTGMSRMLRSYLSSAYENISLWHERDISHSSVERVFIPDATML
TYYMLNKANYLVENLVVHKDRMKENIEKSYNLIYSQRVLLKLVELGVSREEAYKIVQENAMKAWNERRDFKAILLEDNRV
KAKLSDKEAFEDIFSPDYYLKNINEIYERFEL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=391, Percent_Identity=25.0639386189258, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI183227688, Length=405, Percent_Identity=25.679012345679, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1787376, Length=325, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17508577, Length=395, Percent_Identity=27.3417721518987, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32564234, Length=352, Percent_Identity=27.5568181818182, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=390, Percent_Identity=26.6666666666667, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24647570, Length=386, Percent_Identity=23.5751295336788, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49936; Mature: 49936

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIERYSLSPIKDIWTLDAQYKRWLEVEIAVIEAFEELNLAPKGTSQNIRQKAKLDVQKI
CCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LDTEKIVDHDVIAFIKVVTEDMGDEARYFHKGLTSSDVVDTALSLAIKRAEEIILDELTQ
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YIANLKKLAVDHKYTIIVGRTHGVHAEPTSFGLKILGFVAEEERNKERLEKAIDNISQGK
HHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
LSGAVGNYANIEPKVEEIALKKLNLRPCKVSTQVLPRDLHAEFFSVLAMIGSSIERLAIE
HHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IRHLQKTEVLEVEEPFKKEQRGSSAMPHKKNPILCERLTGMSRMLRSYLSSAYENISLWH
HHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERDISHSSVERVFIPDATMLTYYMLNKANYLVENLVVHKDRMKENIEKSYNLIYSQRVLL
HCCCCHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVELGVSREEAYKIVQENAMKAWNERRDFKAILLEDNRVKAKLSDKEAFEDIFSPDYYL
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHHCCCHHHH
KNINEIYERFEL
HHHHHHHHHHCC
>Mature Secondary Structure
MLIERYSLSPIKDIWTLDAQYKRWLEVEIAVIEAFEELNLAPKGTSQNIRQKAKLDVQKI
CCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LDTEKIVDHDVIAFIKVVTEDMGDEARYFHKGLTSSDVVDTALSLAIKRAEEIILDELTQ
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YIANLKKLAVDHKYTIIVGRTHGVHAEPTSFGLKILGFVAEEERNKERLEKAIDNISQGK
HHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
LSGAVGNYANIEPKVEEIALKKLNLRPCKVSTQVLPRDLHAEFFSVLAMIGSSIERLAIE
HHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IRHLQKTEVLEVEEPFKKEQRGSSAMPHKKNPILCERLTGMSRMLRSYLSSAYENISLWH
HHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERDISHSSVERVFIPDATMLTYYMLNKANYLVENLVVHKDRMKENIEKSYNLIYSQRVLL
HCCCCHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVELGVSREEAYKIVQENAMKAWNERRDFKAILLEDNRVKAKLSDKEAFEDIFSPDYYL
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHHCCCHHHH
KNINEIYERFEL
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571; 10673438 [H]