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The map label for this gene is greA

Identifier: 160901647

GI number: 160901647

Start: 159257

End: 159736

Strand: Direct

Name: greA

Synonym: Pmob_0158

Alternate gene names: 160901647

Gene position: 159257-159736 (Clockwise)

Preceding gene: 160901646

Following gene: 160901648

Centisome position: 7.34

GC content: 35.42

Gene sequence:

>480_bases
GTGGCTGAACAAGTATACCAACTAACCCGAGAAGGGTACGAAAAATTAAAGAGGGAACGAGAAGAGCTTAAAAAAAGACT
TATGGGAGAAGTTGCCGAAAGAATTAAAGAAGCCAGGGAATTGGGAGATCTTTCAGAAAACAGTGAGTACGAAGAGGCAA
AAAACGAACAAGGAATGATAGATTCCAGAATCAAAGAGATAGATTATATATTAGACAATGCAGAAATAATTGAAAATGGA
GGCAACCACGATAACTCTACCGTAAGTTTGGGGAAAAAAGTGAAAATAAAAGACTATAGATTGAATAGAGAGCAGGAATT
CGTTCTAGTTACCCCTCAAGAGGCAAATATAGAAGAAAATAAGATCAGTATTGAATCTCCTATTGGAAGATCATTACTAG
GAAGGAAAAAAGGTGAAGTTATCAACATTAAAACCGCCAGAGGAAGTTCAAAAAAAATAGAAATCAAAGAGATAAGCTAA

Upstream 100 bases:

>100_bases
TGTATAAAGAATTTAGAGATTTAGACAGCTTTTTACAAGCTATAAGAGAATTAAAAAATAGTTAATCACCATTATTAGAA
ATGGGAGGAGCGATACAAAA

Downstream 100 bases:

>100_bases
GATTTCATAGATCACAAAACTAACCTTACCAAGCAGGAGGCAAAGTATGGACCTTAGAAATATACGAATGCATCAATTAA
AAGAAATGAGGGAAAAAGGA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MAEQVYQLTREGYEKLKREREELKKRLMGEVAERIKEARELGDLSENSEYEEAKNEQGMIDSRIKEIDYILDNAEIIENG
GNHDNSTVSLGKKVKIKDYRLNREQEFVLVTPQEANIEENKISIESPIGRSLLGRKKGEVINIKTARGSSKKIEIKEIS

Sequences:

>Translated_159_residues
MAEQVYQLTREGYEKLKREREELKKRLMGEVAERIKEARELGDLSENSEYEEAKNEQGMIDSRIKEIDYILDNAEIIENG
GNHDNSTVSLGKKVKIKDYRLNREQEFVLVTPQEANIEENKISIESPIGRSLLGRKKGEVINIKTARGSSKKIEIKEIS
>Mature_158_residues
AEQVYQLTREGYEKLKREREELKKRLMGEVAERIKEARELGDLSENSEYEEAKNEQGMIDSRIKEIDYILDNAEIIENGG
NHDNSTVSLGKKVKIKDYRLNREQEFVLVTPQEANIEENKISIESPIGRSLLGRKKGEVINIKTARGSSKKIEIKEIS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=145, Percent_Identity=38.6206896551724, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87082261, Length=143, Percent_Identity=32.1678321678322, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_PETMO (A9BFC0)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001567228.1
- ProteinModelPortal:   A9BFC0
- SMR:   A9BFC0
- GeneID:   5757930
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_0158
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   PMOB403833:PMOB_0158-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18309; Mature: 18178

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQVYQLTREGYEKLKREREELKKRLMGEVAERIKEARELGDLSENSEYEEAKNEQGMI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHH
DSRIKEIDYILDNAEIIENGGNHDNSTVSLGKKVKIKDYRLNREQEFVLVTPQEANIEEN
HHHHHHHHHHHCCHHHHHCCCCCCCCEEECCCEEEEEEEECCCCCCEEEECCCCCCCCCC
KISIESPIGRSLLGRKKGEVINIKTARGSSKKIEIKEIS
EEEECCCCCHHHHCCCCCCEEEEEECCCCCCEEEEEECC
>Mature Secondary Structure 
AEQVYQLTREGYEKLKREREELKKRLMGEVAERIKEARELGDLSENSEYEEAKNEQGMI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHH
DSRIKEIDYILDNAEIIENGGNHDNSTVSLGKKVKIKDYRLNREQEFVLVTPQEANIEEN
HHHHHHHHHHHCCHHHHHCCCCCCCCEEECCCEEEEEEEECCCCCCEEEECCCCCCCCCC
KISIESPIGRSLLGRKKGEVINIKTARGSSKKIEIKEIS
EEEECCCCCHHHHCCCCCCEEEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA