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The map label for this gene is fixL [H]

Identifier: 160876963

GI number: 160876963

Start: 4545326

End: 4547341

Strand: Reverse

Name: fixL [H]

Synonym: Sbal195_3859

Alternate gene names: 160876963

Gene position: 4547341-4545326 (Counterclockwise)

Preceding gene: 160876968

Following gene: 160876962

Centisome position: 85.04

GC content: 51.19

Gene sequence:

>2016_bases
GTGTTTAGCTCAGGGATGTCACTCAAGTGGCCGTTATCAATTCAAGGTTCAATCGAGCCTAAGATGACACCTCGAATAAT
TTTCAGGATGGCACTTAGGACTAAGGTGTTCATCACGTTATTAGCGTGCTTGTTCGCTTCGGCAAACGTGTTTGCCGTAG
AGCCGCAGAATCAAAGTGCGCCTTCGGATGTAATGGACGCTGAGGTTGTTGCGCCAGTCGCTACCGAAGAACCCGGTTAT
CAAGTGGTCGATGTGGGTGTGTTAGCTATCCGCGGTTATAAGGCGACCATCAACCGCTGGCAGCCGTTAATGGGTTGGCT
CGAAACCCAAATTCCCAACAGTTACTTTAGGTTACATCCTCTCTCCCTCGATGAGTTAGCCAAGGGCGTTGAAACCCAAG
GGCTCGATTTTGTGATCACTAACCCCGGGCAATCCGTGTTGCTGGCGCGGCAATATTCTCTCACTTGGTTGGCAACCCTG
CGCAGCCCGTTAAATAATGGCGCGGCGATGCAAGTGGGTTCGGCGCTGGTGGTGCGCGCCGATTCACCCTATCAGACTTT
AACGGATCTCAAGGGACGCCGACTCGGTATAGTGTCGAAAAATGCCTTCGGCGGTTACTTGACCTTAGTGTACGAAGCGC
AGCTAAAGGGCATAGATTTGCCTCGCTTTGTGGGTGAGATTATTCCGCTGGGGTTCCCGCTAGATAATTTGTTGTATCAG
TTAGATGATCAAAAAAGTGGTAATGATGCGGCGAAAGACAATCGACTGGATGCGACTGTAGTGCCTGTGTGTCAGCTTGA
GCAGATGCAGGCAGAAGGGCTGATTAATATCGGCCATTATCGGGTGCTCGATAACCAAACTCCGGTGGGATTTCATTGCC
AAGTCTCGACTCGCCTCTATCCCAACTGGTCTATGGCGAAGACCAATCGCGCCTCGCAATCTTTAGCTAAAAGCGTGACT
CAAGCGCTGTTGGCGCTGCCCGAAGATCACCTCGCCGCTAAGTCTTCCGATTCTGCGGGTTGGACCACGGCCGTGAGTCA
GCTGGCGATCGACCAACTGCTCAAAGATCTCGACATGCATCCCTTACAAACACCTTGGTGGCAAAGAGCGTGGCAGTGGG
TCAAGTTACATCAACAATGGGCTTGGTTTATTTTGGGGATTTTGGTGCTGCTCAATGCGTACCATTTCTGGTTGGAGTAT
CGTTTTAGCCGTCGCGGCCGGGAGTTGATTAACACTCAGCGCCAGCTCAATGAAAACCGCGCGCTACTGGAACATGCTCA
GCGCATCGCGATTGCGGGTGAGCTTGGCGCTAGCCTCTCCCATGAACTCAATCAACCTTTGGCGGCGATTGGCCATTATT
GCCATGGCGCCGAAGTTCGATTACAGCGGGGGACGAGCCCTGAAGAACTGCAATCGGTATTGACGCTTATCCAGCAGGAA
GTCACGCGCGCCGACAGTATTATCAGCCGCTTACGTAACTTGTTGAAGAAACGGCCTGTATCGAAACAAGCTTTGTACTT
GCATGAGCTGGTTAATGAGACTGTACCGCTGCTCGCCTATGAGTTTGAGCAACATCAAATCAATCTTGCCGTGAATGTGA
GCGGTGAGCCCTATTTACAGTCCCTCGATGAAGTTGGCATGCAGCAGTTGTTGCTTAATTTGCTGAAAAACGCCTTTGAC
GCCTGCGTTCAGCGGTTAGAGCTTGAATCCAGTGGCACAGAACAGAGCGGAATGCGCAAGCCTTATGTTCCGACGATCGA
TATCGATCTTCGCTATCAAGAACGCACACTCTTGCTGACAGTCACAGATAATGGCACTGGGTTAACCGAAGAGACCAGCC
TGCTGATGCAAGCCTTTTATTCCACTAAATCAGAGGGATTAGGTTTAGGCTTAGTGATTTGCCGCGATATTGCCGAGAGC
CATGGCGGAACCTTTAGCCTAGAAAGCGCTATGGGTGGCGGTTGCCAAGCGCAGGTGGCTATACCGCGTAAACCTGAACC
TAATGGAGTACTTTAA

Upstream 100 bases:

>100_bases
CCTACTTTAAACTGGATTTGAGCTTGAGTTGAATTTGAGTTGAATTTGAGTGATTGGGGATAATGGATTGCACTTCACGT
TATTTAGGATGATGAAAACA

Downstream 100 bases:

>100_bases
TGAGTCTTGCCTTACCTGTGTATTTAATCGATGACGATGACTCAGTGCGTCGCTCTTTGCGTTTTATGCTCGAAAGTTAT
GGCCTCAAGATCACCGACTT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MFSSGMSLKWPLSIQGSIEPKMTPRIIFRMALRTKVFITLLACLFASANVFAVEPQNQSAPSDVMDAEVVAPVATEEPGY
QVVDVGVLAIRGYKATINRWQPLMGWLETQIPNSYFRLHPLSLDELAKGVETQGLDFVITNPGQSVLLARQYSLTWLATL
RSPLNNGAAMQVGSALVVRADSPYQTLTDLKGRRLGIVSKNAFGGYLTLVYEAQLKGIDLPRFVGEIIPLGFPLDNLLYQ
LDDQKSGNDAAKDNRLDATVVPVCQLEQMQAEGLINIGHYRVLDNQTPVGFHCQVSTRLYPNWSMAKTNRASQSLAKSVT
QALLALPEDHLAAKSSDSAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQQWAWFILGILVLLNAYHFWLEY
RFSRRGRELINTQRQLNENRALLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQE
VTRADSIISRLRNLLKKRPVSKQALYLHELVNETVPLLAYEFEQHQINLAVNVSGEPYLQSLDEVGMQQLLLNLLKNAFD
ACVQRLELESSGTEQSGMRKPYVPTIDIDLRYQERTLLLTVTDNGTGLTEETSLLMQAFYSTKSEGLGLGLVICRDIAES
HGGTFSLESAMGGGCQAQVAIPRKPEPNGVL

Sequences:

>Translated_671_residues
MFSSGMSLKWPLSIQGSIEPKMTPRIIFRMALRTKVFITLLACLFASANVFAVEPQNQSAPSDVMDAEVVAPVATEEPGY
QVVDVGVLAIRGYKATINRWQPLMGWLETQIPNSYFRLHPLSLDELAKGVETQGLDFVITNPGQSVLLARQYSLTWLATL
RSPLNNGAAMQVGSALVVRADSPYQTLTDLKGRRLGIVSKNAFGGYLTLVYEAQLKGIDLPRFVGEIIPLGFPLDNLLYQ
LDDQKSGNDAAKDNRLDATVVPVCQLEQMQAEGLINIGHYRVLDNQTPVGFHCQVSTRLYPNWSMAKTNRASQSLAKSVT
QALLALPEDHLAAKSSDSAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQQWAWFILGILVLLNAYHFWLEY
RFSRRGRELINTQRQLNENRALLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQE
VTRADSIISRLRNLLKKRPVSKQALYLHELVNETVPLLAYEFEQHQINLAVNVSGEPYLQSLDEVGMQQLLLNLLKNAFD
ACVQRLELESSGTEQSGMRKPYVPTIDIDLRYQERTLLLTVTDNGTGLTEETSLLMQAFYSTKSEGLGLGLVICRDIAES
HGGTFSLESAMGGGCQAQVAIPRKPEPNGVL
>Mature_671_residues
MFSSGMSLKWPLSIQGSIEPKMTPRIIFRMALRTKVFITLLACLFASANVFAVEPQNQSAPSDVMDAEVVAPVATEEPGY
QVVDVGVLAIRGYKATINRWQPLMGWLETQIPNSYFRLHPLSLDELAKGVETQGLDFVITNPGQSVLLARQYSLTWLATL
RSPLNNGAAMQVGSALVVRADSPYQTLTDLKGRRLGIVSKNAFGGYLTLVYEAQLKGIDLPRFVGEIIPLGFPLDNLLYQ
LDDQKSGNDAAKDNRLDATVVPVCQLEQMQAEGLINIGHYRVLDNQTPVGFHCQVSTRLYPNWSMAKTNRASQSLAKSVT
QALLALPEDHLAAKSSDSAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQQWAWFILGILVLLNAYHFWLEY
RFSRRGRELINTQRQLNENRALLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQE
VTRADSIISRLRNLLKKRPVSKQALYLHELVNETVPLLAYEFEQHQINLAVNVSGEPYLQSLDEVGMQQLLLNLLKNAFD
ACVQRLELESSGTEQSGMRKPYVPTIDIDLRYQERTLLLTVTDNGTGLTEETSLLMQAFYSTKSEGLGLGLVICRDIAES
HGGTFSLESAMGGGCQAQVAIPRKPEPNGVL

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=292, Percent_Identity=25.3424657534247, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788549, Length=254, Percent_Identity=26.3779527559055, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 74645; Mature: 74645

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSGMSLKWPLSIQGSIEPKMTPRIIFRMALRTKVFITLLACLFASANVFAVEPQNQSA
CCCCCCCEEECEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
PSDVMDAEVVAPVATEEPGYQVVDVGVLAIRGYKATINRWQPLMGWLETQIPNSYFRLHP
CCHHCCHHHCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECC
LSLDELAKGVETQGLDFVITNPGQSVLLARQYSLTWLATLRSPLNNGAAMQVGSALVVRA
CCHHHHHCCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHCCCCCCEEECCEEEEEC
DSPYQTLTDLKGRRLGIVSKNAFGGYLTLVYEAQLKGIDLPRFVGEIIPLGFPLDNLLYQ
CCCHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHH
LDDQKSGNDAAKDNRLDATVVPVCQLEQMQAEGLINIGHYRVLDNQTPVGFHCQVSTRLY
HCCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCEECCEEEEECCCCCCCEEEEEECEEC
PNWSMAKTNRASQSLAKSVTQALLALPEDHLAAKSSDSAGWTTAVSQLAIDQLLKDLDMH
CCCCCHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
PLQTPWWQRAWQWVKLHQQWAWFILGILVLLNAYHFWLEYRFSRRGRELINTQRQLNENR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHH
ALLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQE
HHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
VTRADSIISRLRNLLKKRPVSKQALYLHELVNETVPLLAYEFEQHQINLAVNVSGEPYLQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHEEEECCEEEEEEEECCCCHHHH
SLDEVGMQQLLLNLLKNAFDACVQRLELESSGTEQSGMRKPYVPTIDIDLRYQERTLLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECEEEEEE
VTDNGTGLTEETSLLMQAFYSTKSEGLGLGLVICRDIAESHGGTFSLESAMGGGCQAQVA
EECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEE
IPRKPEPNGVL
CCCCCCCCCCC
>Mature Secondary Structure
MFSSGMSLKWPLSIQGSIEPKMTPRIIFRMALRTKVFITLLACLFASANVFAVEPQNQSA
CCCCCCCEEECEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
PSDVMDAEVVAPVATEEPGYQVVDVGVLAIRGYKATINRWQPLMGWLETQIPNSYFRLHP
CCHHCCHHHCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECC
LSLDELAKGVETQGLDFVITNPGQSVLLARQYSLTWLATLRSPLNNGAAMQVGSALVVRA
CCHHHHHCCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHCCCCCCEEECCEEEEEC
DSPYQTLTDLKGRRLGIVSKNAFGGYLTLVYEAQLKGIDLPRFVGEIIPLGFPLDNLLYQ
CCCHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHH
LDDQKSGNDAAKDNRLDATVVPVCQLEQMQAEGLINIGHYRVLDNQTPVGFHCQVSTRLY
HCCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCEECCEEEEECCCCCCCEEEEEECEEC
PNWSMAKTNRASQSLAKSVTQALLALPEDHLAAKSSDSAGWTTAVSQLAIDQLLKDLDMH
CCCCCHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
PLQTPWWQRAWQWVKLHQQWAWFILGILVLLNAYHFWLEYRFSRRGRELINTQRQLNENR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHH
ALLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQE
HHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
VTRADSIISRLRNLLKKRPVSKQALYLHELVNETVPLLAYEFEQHQINLAVNVSGEPYLQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHEEEECCEEEEEEEECCCCHHHH
SLDEVGMQQLLLNLLKNAFDACVQRLELESSGTEQSGMRKPYVPTIDIDLRYQERTLLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECEEEEEE
VTDNGTGLTEETSLLMQAFYSTKSEGLGLGLVICRDIAESHGGTFSLESAMGGGCQAQVA
EECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEE
IPRKPEPNGVL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]