Click here to switch to the map view.

The map label for this gene is fixJ [H]

Identifier: 160876962

GI number: 160876962

Start: 4544706

End: 4545326

Strand: Reverse

Name: fixJ [H]

Synonym: Sbal195_3858

Alternate gene names: 160876962

Gene position: 4545326-4544706 (Counterclockwise)

Preceding gene: 160876963

Following gene: 160876961

Centisome position: 85.0

GC content: 50.56

Gene sequence:

>621_bases
ATGAGTCTTGCCTTACCTGTGTATTTAATCGATGACGATGACTCAGTGCGTCGCTCTTTGCGTTTTATGCTCGAAAGTTA
TGGCCTCAAGATCACCGACTTTGATAGCGCCGAAGCCTTTTTTACCGCCGTAGACTTAACCCTACCGGGTTGTGCGCTGG
TTGATGTGCGTATGCCAGGCTTGAGCGGCCCGCAGTTGCATCTTGAATTAGTCTCCAAAAATAGTCCGTTAGCTGTGATT
TATTTGACCGGACATGGCGATGTGCCTATGGCGGTTGAAGCGCTCAAACTCGGTGCGGTGGATTTTTTCCAAAAGCCCGC
CGATGGCGCTAAGTTAGCCGAAGCGGTCGTCAAAGCGTTGGAACATGCTAAAACGCATTATCAAGACAATCAGTATCTTG
AGACGTATCAAGCGCTCACGCCAAGGGAGCGGGAGATACTCAACCTGATTGCCCAAGGGTTGAAGAACCAAGAGATCGCC
GATAACCTCTGTATTGCCATGCGTACGGTCGAGATCCACCGTGCCAATTTGATGAAGGGCATGCAAGTAGGATCGCTGGC
GGAAATGATGCTGATCTATAGCCGGATCGCCGAACGCCTGCGCCTTGAAGATAACCGCTAA

Upstream 100 bases:

>100_bases
AGAGCCATGGCGGAACCTTTAGCCTAGAAAGCGCTATGGGTGGCGGTTGCCAAGCGCAGGTGGCTATACCGCGTAAACCT
GAACCTAATGGAGTACTTTA

Downstream 100 bases:

>100_bases
AATTTACCTTTTACTGCGCTAATTGCCGCTTTGTTAGCCGCCTATTTTGGCTAAGTTGTCGTATCATTGGCGGTTAGTAT
TGGCTTTGCTAGTAGCTTAG

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSLALPVYLIDDDDSVRRSLRFMLESYGLKITDFDSAEAFFTAVDLTLPGCALVDVRMPGLSGPQLHLELVSKNSPLAVI
YLTGHGDVPMAVEALKLGAVDFFQKPADGAKLAEAVVKALEHAKTHYQDNQYLETYQALTPREREILNLIAQGLKNQEIA
DNLCIAMRTVEIHRANLMKGMQVGSLAEMMLIYSRIAERLRLEDNR

Sequences:

>Translated_206_residues
MSLALPVYLIDDDDSVRRSLRFMLESYGLKITDFDSAEAFFTAVDLTLPGCALVDVRMPGLSGPQLHLELVSKNSPLAVI
YLTGHGDVPMAVEALKLGAVDFFQKPADGAKLAEAVVKALEHAKTHYQDNQYLETYQALTPREREILNLIAQGLKNQEIA
DNLCIAMRTVEIHRANLMKGMQVGSLAEMMLIYSRIAERLRLEDNR
>Mature_205_residues
SLALPVYLIDDDDSVRRSLRFMLESYGLKITDFDSAEAFFTAVDLTLPGCALVDVRMPGLSGPQLHLELVSKNSPLAVIY
LTGHGDVPMAVEALKLGAVDFFQKPADGAKLAEAVVKALEHAKTHYQDNQYLETYQALTPREREILNLIAQGLKNQEIAD
NLCIAMRTVEIHRANLMKGMQVGSLAEMMLIYSRIAERLRLEDNR

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=135, Percent_Identity=35.5555555555556, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22949; Mature: 22818

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLALPVYLIDDDDSVRRSLRFMLESYGLKITDFDSAEAFFTAVDLTLPGCALVDVRMPG
CCCEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCHHHEEEEEECCCCEEEEEEECCC
LSGPQLHLELVSKNSPLAVIYLTGHGDVPMAVEALKLGAVDFFQKPADGAKLAEAVVKAL
CCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EHAKTHYQDNQYLETYQALTPREREILNLIAQGLKNQEIADNLCIAMRTVEIHRANLMKG
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
MQVGSLAEMMLIYSRIAERLRLEDNR
CCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLALPVYLIDDDDSVRRSLRFMLESYGLKITDFDSAEAFFTAVDLTLPGCALVDVRMPG
CCEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCHHHEEEEEECCCCEEEEEEECCC
LSGPQLHLELVSKNSPLAVIYLTGHGDVPMAVEALKLGAVDFFQKPADGAKLAEAVVKAL
CCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EHAKTHYQDNQYLETYQALTPREREILNLIAQGLKNQEIADNLCIAMRTVEIHRANLMKG
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
MQVGSLAEMMLIYSRIAERLRLEDNR
CCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]