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The map label for this gene is pflB [H]

Identifier: 160875880

GI number: 160875880

Start: 3298336

End: 3300618

Strand: Direct

Name: pflB [H]

Synonym: Sbal195_2769

Alternate gene names: 160875880

Gene position: 3298336-3300618 (Clockwise)

Preceding gene: 160875878

Following gene: 160875881

Centisome position: 61.68

GC content: 46.61

Gene sequence:

>2283_bases
ATGACCGAAAAAACTGAACTGTTTGCCAACGCATGGGAAGGCTTTACCCCTGGCGATTGGAAATCTGAAGTCAATGTACG
TGACTTTATCCAACAAAACTACGCGCCTTTTGAAGGCGACGAATCATTCCTTGCCGGTGCCACCGAAGCGACCACAACCC
TGTGGGATAAAGTCATGGAAGGCATCAAACAGGAAAACCGTACCCACGCGCCTGTTGATTTCGATACTAAGATGGTTTCT
ACCATCACTTCCCACGACGCTGGCTACATCAACAAAGAATTAGAAACTATCGTTGGCTTACAAACAGATGCGCCGCTAAA
ACGCGCTATGTTGCCTAACGGTGGTATTCGTATGGTGGAAAGCTCATGCGCTGCCTACGACCGCGTGCTAGATGAAGACG
TTAAATACATCTATTCAGAACTGCGTAAAACCCATAACCAAGGTGTATTCGATGTTTACACCCCAGAAATTATGGCTTGC
CGTAAATCAGGTATTTTAACCGGTTTACCTGATGCTTATGGCCGTGGCCGTATCATTGGTGACTATCGCCGTGTTGCACT
TTACGGTATCGATTACCTGATGAAAGATAAGTTTGCCCAATTCACTTCACTGCAAGCGCAGTTCGAAGCGGGTGAAGAAT
TATCAAATGTTATCCAATTACGTGAAGAAATCGCTGAGCAACACCGTGCACTAGGTCAAATGAAGAAAATGGCTGCCAAA
TATGGCTGCGATATTTCTCGCCCAGCAACCACTGCACAAGAAGCTATTCAATGGACTTACTTCGGTTACTTAGCCGCAGT
GAAAAGCCAAAACGGCGCGGCGATGTCATTAGGCCGTACCTCTTCATTCCTCGACATCTATATTGAACGCGATCTGAAAA
ATGGCACCTTAACTGAGCAACAAGCTCAGGAAATGATTGACCATTTCGTGATGAAACTGCGTATGGTACGTTTCCTACGT
ACTCCAGAATACGATGAATTGTTCTCTGGCGACCCAATTTGGGCGACTGAGTCAATTGGCGGTATGGGCTTAGATGGCCG
TACGTTAGTGACTAAATCAAGCTTCCGTTTCTTGAATACCCTTTACACTATGGGTCCAAGTCCAGAGCCAAACATCACAG
TATTGTGGTCAGATAAACTGCCCATCGGCTTCAAAAAATATTGCGCCAAAGTGTCTATCGACACTAGCTCAATCCAATAT
GAAAACGATGACCTAATGCGTCCAGATTTCCAATCAGACGACTATGCTATTGCCTGTTGTGTAAGCCCAATGGTTGTGGG
TAAACACATGCAGTTCTTCGGTGCCCGCGCCAACTTAGCTAAAACTATGTTGTATGCGATTAACGGCGGCGTTGACGAAA
AGCTGAAAATCCAAGTTGCCCCTAAAGCCGACCCTATCACTGACGCCGTATTAAACTACGACGACGTGATGAATCGCTTA
GACGGTTTAATGGATTGGTTAGCGACTCAATATGTCACAGCACTGAACTCAATCCACTTCATGCACGACAAGTACTCTTA
CGAAGCCGCGCTAATGGCACTGCACGATAGAGACGTACGTCGCACTATGGCTTGTGGTATTGCGGGTCTGTCTATCGCAG
CCGACTCACTGTCTGCCATTAAATTCGCCACAGTAAAACCTGTTCGTGATGAAAACGGCATTGCAGTTGATTTCGAAATT
AGCGGTGACTATCCAAAATTTGGTAACAACGACCCACGTGTTGATGATATCGCCTGTGATTTAGTTGAACGTTTTATGGC
GAAAATTCGCGACCGTAAAATGTATCGCAATGCGATCCCAACTCAGTCAATTCTGACCATCACCTCAAACGTGGTTTATG
GTAAGAAAACCGGTAACACACCAGATGGTCGTCGTTCAGGTGCGCCATTTGCACCGGGTGCAAACCCTATGCATGGCCGT
GATGAAAAAGGCGCAATCGCTTCTTTAACCTCAGTGGCAAAACTCCCATTTGCCCACGCTCAAGATGGTATCTCTTATAC
CTTCTCAATCGTGCCAAATGCCCTGGGTAAAGATGATGATGCTCGCCGTACCAACTTAGCGGCATTGATGGACGGATACT
TCGACCACAATGACAAAAAAGAAGGTGGCCAACACTTGAACGTCAACGTGATGAACCGCGAGATGCTCGAAGATGCCGTT
AAGCATCCAGACAAGTATCCACAGCTGACCATCCGTGTTTCTGGCTACGCAGTGCGCTTCAACTCACTGACGCCAGAGCA
GCAACAAGATGTGATCACGCGTACATTCACCAAAGGTCTGTAA

Upstream 100 bases:

>100_bases
GAAGGGAGCTTTATTTCAGGAATTAGCTATTGGCTTATACTCAGCATCGCTTCACGCTGATCGCCAATAACCTTTATCTC
AACCAGAAGGTATTAGTACG

Downstream 100 bases:

>100_bases
GCTAAATTAGTCAAGGCTGTAGTACACTGTGCTACAGCCTCACTTATTTTAACAGGAGTCACAATGGCAGTTATCGGTCG
GATCCACTCGGTGGAATCCT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MTEKTELFANAWEGFTPGDWKSEVNVRDFIQQNYAPFEGDESFLAGATEATTTLWDKVMEGIKQENRTHAPVDFDTKMVS
TITSHDAGYINKELETIVGLQTDAPLKRAMLPNGGIRMVESSCAAYDRVLDEDVKYIYSELRKTHNQGVFDVYTPEIMAC
RKSGILTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKFAQFTSLQAQFEAGEELSNVIQLREEIAEQHRALGQMKKMAAK
YGCDISRPATTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTLTEQQAQEMIDHFVMKLRMVRFLR
TPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNTLYTMGPSPEPNITVLWSDKLPIGFKKYCAKVSIDTSSIQY
ENDDLMRPDFQSDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQVAPKADPITDAVLNYDDVMNRL
DGLMDWLATQYVTALNSIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAIKFATVKPVRDENGIAVDFEI
SGDYPKFGNNDPRVDDIACDLVERFMAKIRDRKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGR
DEKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDDDARRTNLAALMDGYFDHNDKKEGGQHLNVNVMNREMLEDAV
KHPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL

Sequences:

>Translated_760_residues
MTEKTELFANAWEGFTPGDWKSEVNVRDFIQQNYAPFEGDESFLAGATEATTTLWDKVMEGIKQENRTHAPVDFDTKMVS
TITSHDAGYINKELETIVGLQTDAPLKRAMLPNGGIRMVESSCAAYDRVLDEDVKYIYSELRKTHNQGVFDVYTPEIMAC
RKSGILTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKFAQFTSLQAQFEAGEELSNVIQLREEIAEQHRALGQMKKMAAK
YGCDISRPATTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTLTEQQAQEMIDHFVMKLRMVRFLR
TPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNTLYTMGPSPEPNITVLWSDKLPIGFKKYCAKVSIDTSSIQY
ENDDLMRPDFQSDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQVAPKADPITDAVLNYDDVMNRL
DGLMDWLATQYVTALNSIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAIKFATVKPVRDENGIAVDFEI
SGDYPKFGNNDPRVDDIACDLVERFMAKIRDRKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGR
DEKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDDDARRTNLAALMDGYFDHNDKKEGGQHLNVNVMNREMLEDAV
KHPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL
>Mature_759_residues
TEKTELFANAWEGFTPGDWKSEVNVRDFIQQNYAPFEGDESFLAGATEATTTLWDKVMEGIKQENRTHAPVDFDTKMVST
ITSHDAGYINKELETIVGLQTDAPLKRAMLPNGGIRMVESSCAAYDRVLDEDVKYIYSELRKTHNQGVFDVYTPEIMACR
KSGILTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKFAQFTSLQAQFEAGEELSNVIQLREEIAEQHRALGQMKKMAAKY
GCDISRPATTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTLTEQQAQEMIDHFVMKLRMVRFLRT
PEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNTLYTMGPSPEPNITVLWSDKLPIGFKKYCAKVSIDTSSIQYE
NDDLMRPDFQSDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQVAPKADPITDAVLNYDDVMNRLD
GLMDWLATQYVTALNSIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAIKFATVKPVRDENGIAVDFEIS
GDYPKFGNNDPRVDDIACDLVERFMAKIRDRKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGRD
EKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDDDARRTNLAALMDGYFDHNDKKEGGQHLNVNVMNREMLEDAVK
HPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=760, Percent_Identity=81.8421052631579, Blast_Score=1337, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=755, Percent_Identity=75.4966887417219, Blast_Score=1231, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=595, Percent_Identity=27.8991596638655, Blast_Score=189, Evalue=6e-49,
Organism=Escherichia coli, GI1790388, Length=665, Percent_Identity=24.3609022556391, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788933, Length=60, Percent_Identity=86.6666666666667, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84907; Mature: 84776

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKTELFANAWEGFTPGDWKSEVNVRDFIQQNYAPFEGDESFLAGATEATTTLWDKVME
CCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHH
GIKQENRTHAPVDFDTKMVSTITSHDAGYINKELETIVGLQTDAPLKRAMLPNGGIRMVE
HHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHH
SSCAAYDRVLDEDVKYIYSELRKTHNQGVFDVYTPEIMACRKSGILTGLPDAYGRGRIIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCEEH
DYRRVALYGIDYLMKDKFAQFTSLQAQFEAGEELSNVIQLREEIAEQHRALGQMKKMAAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGCDISRPATTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTLTEQ
HCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCCCHH
QAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNT
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEHHHHHHHHH
LYTMGPSPEPNITVLWSDKLPIGFKKYCAKVSIDTSSIQYENDDLMRPDFQSDDYAIACC
HHHCCCCCCCCEEEEECCCCCCCHHHHHHEEEECCCCEEECCCCCCCCCCCCCCCEEEEE
VSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQVAPKADPITDAVLNYDDVMNRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCHHHHHHHH
DGLMDWLATQYVTALNSIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHE
KFATVKPVRDENGIAVDFEISGDYPKFGNNDPRVDDIACDLVERFMAKIRDRKMYRNAIP
EEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGRDEKGAIASLTSVAKLPFAHA
CHHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHC
QDGISYTFSIVPNALGKDDDARRTNLAALMDGYFDHNDKKEGGQHLNVNVMNREMLEDAV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHH
KHPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL
HCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEKTELFANAWEGFTPGDWKSEVNVRDFIQQNYAPFEGDESFLAGATEATTTLWDKVME
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHH
GIKQENRTHAPVDFDTKMVSTITSHDAGYINKELETIVGLQTDAPLKRAMLPNGGIRMVE
HHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHH
SSCAAYDRVLDEDVKYIYSELRKTHNQGVFDVYTPEIMACRKSGILTGLPDAYGRGRIIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCEEH
DYRRVALYGIDYLMKDKFAQFTSLQAQFEAGEELSNVIQLREEIAEQHRALGQMKKMAAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGCDISRPATTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTLTEQ
HCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCCCHH
QAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNT
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEHHHHHHHHH
LYTMGPSPEPNITVLWSDKLPIGFKKYCAKVSIDTSSIQYENDDLMRPDFQSDDYAIACC
HHHCCCCCCCCEEEEECCCCCCCHHHHHHEEEECCCCEEECCCCCCCCCCCCCCCEEEEE
VSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQVAPKADPITDAVLNYDDVMNRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCHHHHHHHH
DGLMDWLATQYVTALNSIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHE
KFATVKPVRDENGIAVDFEISGDYPKFGNNDPRVDDIACDLVERFMAKIRDRKMYRNAIP
EEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGRDEKGAIASLTSVAKLPFAHA
CHHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHC
QDGISYTFSIVPNALGKDDDARRTNLAALMDGYFDHNDKKEGGQHLNVNVMNREMLEDAV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHH
KHPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL
HCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]