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The map label for this gene is pflA [H]

Identifier: 160875881

GI number: 160875881

Start: 3300682

End: 3301422

Strand: Direct

Name: pflA [H]

Synonym: Sbal195_2770

Alternate gene names: 160875881

Gene position: 3300682-3301422 (Clockwise)

Preceding gene: 160875880

Following gene: 160875883

Centisome position: 61.73

GC content: 45.48

Gene sequence:

>741_bases
ATGGCAGTTATCGGTCGGATCCACTCGGTGGAATCCTTTGGCACAGTCGATGGTCCAGGCATACGGTTTATCACCTTTAT
GCAAGGCTGTTTAATGCGCTGCCAATATTGCCACAACCGTGACACTTGGGATCTTGATGGCGGAAAAGAAGTTCTGGTCG
ATGAGTTAATGAGTCAAATCATCAGCTATCGGCCTTTTCTCGATGCCAGCAATGGTGGTGTTACGGCCAGTGGTGGTGAA
GCCATTTTACAGGCAGAATTCGTCGCCGAATTATTCACAGCCTGTCAGCAAGAAGGTATTCATACCTGTTTAGATACCAA
TGGTTTTGTACGTAAGTACACGCCAGTGATTGATGAGTTACTCGACCACACTAACTTGGTCCTGCTCGACATCAAACACA
TGGACGATGAAAAACATATTGAGTTAACTAAAGTCAGCAACCATAGAACACTGCAATTTGCGCAGTATTTGGCAGAGCGT
AATCAAGCGACTTGGATACGGTATGTGGTGGTGGGCGGATTTACCGACGATGAAGCATCGGCATTACAACTGGCCGAGTT
TATCAAGCCCATGAAAAACATTGAAAAGGTAGAATTACTACCCTATCACGAATTAGGTAAACACAAGTGGGAAGCCATGG
GCGAAAGCTATCAACTCGATGGCATATCACCACCGAGCCGAGAAACCATGGAAAAGATCAAAGCCGTATTTGTTGGGCAA
GGGATTAACGCGATTTATTAA

Upstream 100 bases:

>100_bases
AGATGTGATCACGCGTACATTCACCAAAGGTCTGTAAGCTAAATTAGTCAAGGCTGTAGTACACTGTGCTACAGCCTCAC
TTATTTTAACAGGAGTCACA

Downstream 100 bases:

>100_bases
TCCACAGTAATAGAAAAGCCAACACGGATAACAGCGGCAACGGCCTAAAGTTCTTCCCAATAACTGGCATCTAAACGCTC
AAAGGCTATTTTTAAAATCA

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAVIGRIHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEVLVDELMSQIISYRPFLDASNGGVTASGGE
AILQAEFVAELFTACQQEGIHTCLDTNGFVRKYTPVIDELLDHTNLVLLDIKHMDDEKHIELTKVSNHRTLQFAQYLAER
NQATWIRYVVVGGFTDDEASALQLAEFIKPMKNIEKVELLPYHELGKHKWEAMGESYQLDGISPPSRETMEKIKAVFVGQ
GINAIY

Sequences:

>Translated_246_residues
MAVIGRIHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEVLVDELMSQIISYRPFLDASNGGVTASGGE
AILQAEFVAELFTACQQEGIHTCLDTNGFVRKYTPVIDELLDHTNLVLLDIKHMDDEKHIELTKVSNHRTLQFAQYLAER
NQATWIRYVVVGGFTDDEASALQLAEFIKPMKNIEKVELLPYHELGKHKWEAMGESYQLDGISPPSRETMEKIKAVFVGQ
GINAIY
>Mature_245_residues
AVIGRIHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEVLVDELMSQIISYRPFLDASNGGVTASGGEA
ILQAEFVAELFTACQQEGIHTCLDTNGFVRKYTPVIDELLDHTNLVLLDIKHMDDEKHIELTKVSNHRTLQFAQYLAERN
QATWIRYVVVGGFTDDEASALQLAEFIKPMKNIEKVELLPYHELGKHKWEAMGESYQLDGISPPSRETMEKIKAVFVGQG
INAIY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=67.479674796748, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1790389, Length=255, Percent_Identity=25.8823529411765, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1790839, Length=273, Percent_Identity=27.4725274725275, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI226510931, Length=270, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27703; Mature: 27572

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIGRIHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEVLVDELMSQI
CCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
ISYRPFLDASNGGVTASGGEAILQAEFVAELFTACQQEGIHTCLDTNGFVRKYTPVIDEL
HHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LDHTNLVLLDIKHMDDEKHIELTKVSNHRTLQFAQYLAERNQATWIRYVVVGGFTDDEAS
HCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHH
ALQLAEFIKPMKNIEKVELLPYHELGKHKWEAMGESYQLDGISPPSRETMEKIKAVFVGQ
HHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCC
GINAIY
CCHHCC
>Mature Secondary Structure 
AVIGRIHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEVLVDELMSQI
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
ISYRPFLDASNGGVTASGGEAILQAEFVAELFTACQQEGIHTCLDTNGFVRKYTPVIDEL
HHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LDHTNLVLLDIKHMDDEKHIELTKVSNHRTLQFAQYLAERNQATWIRYVVVGGFTDDEAS
HCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHH
ALQLAEFIKPMKNIEKVELLPYHELGKHKWEAMGESYQLDGISPPSRETMEKIKAVFVGQ
HHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCC
GINAIY
CCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]