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The map label for this gene is nuoE2 [H]

Identifier: 159899602

GI number: 159899602

Start: 3892275

End: 3892799

Strand: Direct

Name: nuoE2 [H]

Synonym: Haur_3083

Alternate gene names: 159899602

Gene position: 3892275-3892799 (Clockwise)

Preceding gene: 159899601

Following gene: 159899603

Centisome position: 61.33

GC content: 50.1

Gene sequence:

>525_bases
GTGCTGTACGAACAACACAAGGCTGAAATTGATGAAATTTTGGCCCGCTATCCTGTAGATCGCAAACGCTCGGCCTTGTT
GCCGTTGTTATATTTGGCGCAAGATGTCTATGGCCGCTTGGATCGCGATTCAATTCGCGAAGTGGCCGAGATTCTCGATT
TGCCCTATACCGATGTTTTTGAGGTCGTAGGTTTTTATACACTCTTTTATAACGAAGAAGTTGGCAAGGTTGTGCTCGAT
GTCTGCGACGACGTGCCATGCTGCTTCTGTGGTGCTGAAGAGTTGGTCGCCGATTTGGAAAATCGCCTAGGAATCAAAGC
TGGCGAAACCACCAAAGATAAGGTTTTCACCTTGCGCCGCGTGAAATGTATTGCTGCTTGCGATCAAGCCCCAGTGCTGC
AAGCCAACTTAGAGTTTCACAACCGCGTCTTGCCCGATAAAGTTGAAGCCATGCTCGCCAAGCTGCGCAACGATGTCGAA
TCTGGCAAGCCCGTGAGCATCTCTGGCCGCCTTGCAGAACGCTAA

Upstream 100 bases:

>100_bases
CCCCACATCGCCAAAGGCAAATTGATGGCCGACTTGGTGGCATTGATTGCGAGCATCGATCCGGTGCTTGGTGAAGTGGA
TCGTTAAGAAGGAGCTTAGG

Downstream 100 bases:

>100_bases
ATCAAGTCAAATGGCAAAGGGCAAAAGGCAAAAAGTTTAATTTATCGAACTTCGAGCTTTTGACTTTTGACTTCTGACTT
CTGACTTTTGGAGTGCTTAT

Product: NADH-quinone oxidoreductase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MLYEQHKAEIDEILARYPVDRKRSALLPLLYLAQDVYGRLDRDSIREVAEILDLPYTDVFEVVGFYTLFYNEEVGKVVLD
VCDDVPCCFCGAEELVADLENRLGIKAGETTKDKVFTLRRVKCIAACDQAPVLQANLEFHNRVLPDKVEAMLAKLRNDVE
SGKPVSISGRLAER

Sequences:

>Translated_174_residues
MLYEQHKAEIDEILARYPVDRKRSALLPLLYLAQDVYGRLDRDSIREVAEILDLPYTDVFEVVGFYTLFYNEEVGKVVLD
VCDDVPCCFCGAEELVADLENRLGIKAGETTKDKVFTLRRVKCIAACDQAPVLQANLEFHNRVLPDKVEAMLAKLRNDVE
SGKPVSISGRLAER
>Mature_174_residues
MLYEQHKAEIDEILARYPVDRKRSALLPLLYLAQDVYGRLDRDSIREVAEILDLPYTDVFEVVGFYTLFYNEEVGKVVLD
VCDDVPCCFCGAEELVADLENRLGIKAGETTKDKVFTLRRVKCIAACDQAPVLQANLEFHNRVLPDKVEAMLAKLRNDVE
SGKPVSISGRLAER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=163, Percent_Identity=36.8098159509202, Blast_Score=115, Evalue=2e-26,
Organism=Escherichia coli, GI1788621, Length=134, Percent_Identity=31.3432835820896, Blast_Score=84, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17561328, Length=171, Percent_Identity=42.1052631578947, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI18859877, Length=169, Percent_Identity=37.2781065088757, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355353, Length=153, Percent_Identity=35.2941176470588, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19695; Mature: 19695

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYEQHKAEIDEILARYPVDRKRSALLPLLYLAQDVYGRLDRDSIREVAEILDLPYTDVF
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHH
EVVGFYTLFYNEEVGKVVLDVCDDVPCCFCGAEELVADLENRLGIKAGETTKDKVFTLRR
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VKCIAACDQAPVLQANLEFHNRVLPDKVEAMLAKLRNDVESGKPVSISGRLAER
HHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure
MLYEQHKAEIDEILARYPVDRKRSALLPLLYLAQDVYGRLDRDSIREVAEILDLPYTDVF
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHH
EVVGFYTLFYNEEVGKVVLDVCDDVPCCFCGAEELVADLENRLGIKAGETTKDKVFTLRR
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VKCIAACDQAPVLQANLEFHNRVLPDKVEAMLAKLRNDVESGKPVSISGRLAER
HHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]