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The map label for this gene is nuoF2 [H]

Identifier: 159899603

GI number: 159899603

Start: 3892898

End: 3894166

Strand: Direct

Name: nuoF2 [H]

Synonym: Haur_3084

Alternate gene names: 159899603

Gene position: 3892898-3894166 (Clockwise)

Preceding gene: 159899602

Following gene: 159899604

Centisome position: 61.34

GC content: 51.77

Gene sequence:

>1269_bases
ATGCCTAAGACAGAACTCAAAGAATATATTGTGATGCGCAATCGCGAAATTGAAAATATTCGCGATCTTGATGTGTATCT
GGCAAGTGGTGGGTATGCGATGGCCAAGCAAGCCTTGACCAGCATGACCCCAGCCGCAATTATCGACGAAGTAAAAAAAT
CGGGCTTGCGCGGTCGTGGCGGCGCTGGCTTCCCGACTGGGGTTAAATGGAGCTTCGTTCCCAAGGAATTAAACCCCAAA
TATTTGGTGGTCAACGCCGATGAGAGCGAGCCAGGCACGTTCAACAACCATGAAATTATCGACGAAAATCCCCATCAATT
GCTCGAAGGGATTGTAATCAGTGCCTTTGCAATTGGCGCGAACGTCGCCTATATCTACATTCGCGGCGAATTTGCCTATG
GCGCACGTTTCCTTGAGCAAAAAATTGCTGAAGCTCGCGCCCGTGGCTTGATCGGCAAGAATTTGTTTGGCACTGGCTAC
GATGTGGAAATTTATGTCCATCGTGGAGCTGGCGCTTACATTTGTGGCGAAGAAACCGCCTTGCTCGAATCGCTCGAAGG
CAAAATCGGCCAGCCACGTTTGAAGCCACCCTTCCCTGCTGTCGCTGGTTTGTATGCCAAGCCAACCGTGGTCAACAACG
TTGAAACCTTGACCAACGTGCCACGGATTATCGAAAAAGGCGCTGATTGGTTCCGCTCGTTCGGCACCGAAAAATCGCCT
GGCACCAAAGCAGTTTCAATTAGCGGCCACGTCAAAAAACCTGGCAACTATGAAATTCCCTTGGGCATCACGATTCGCGA
GTTCATTTTCGATTGGGCTGGCGGTATGCGCGACCCTAATTTGCCATTGAAGTTCATTATCCCAGGCGGTGCTTCATCCA
ACTGGCTAACCGAGCAACACCTCGATTTGCCAATGACGTGGGATGATATGGCCGCTGCGGGAACGATGCTCGGCTCAGGC
GCGATGGTCGTGCTCGATACCTCGGTTCCAGTGGTGCGAGCAGCCTTGAAGGTTGACGAATTCTTCAAACACGAATCGTG
TGGCAAGTGTTCGCCCTGTCGCGAAGGCACGCACTTCTTGGTCAAGGTGTGGGAGCGGATCGACGAGGGCCACGGGCGGG
TTGGCGATATTGAATTATTGGCTGATGTTGGCAAGCAAATGCTTGGCAAGTGTTTCTGCCCACTGGGCGATTCATCAGTT
TCAGCCGTCAATAGCGCGATCAAATTCTTCCGGCCTGAGCTTGATGCAGCGATCGATGCACAACACTAA

Upstream 100 bases:

>100_bases
AAATCAAGTCAAATGGCAAAGGGCAAAAGGCAAAAAGTTTAATTTATCGAACTTCGAGCTTTTGACTTTTGACTTCTGAC
TTCTGACTTTTGGAGTGCTT

Downstream 100 bases:

>100_bases
GCATTATTGATCGTTTCAGTTGAGATATCGCGCAAATTTTTAGCCACAGATTGACACAGATGACACAGATGACCATCGAC
TGGCATTGTGCGAATCACCA

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MPKTELKEYIVMRNREIENIRDLDVYLASGGYAMAKQALTSMTPAAIIDEVKKSGLRGRGGAGFPTGVKWSFVPKELNPK
YLVVNADESEPGTFNNHEIIDENPHQLLEGIVISAFAIGANVAYIYIRGEFAYGARFLEQKIAEARARGLIGKNLFGTGY
DVEIYVHRGAGAYICGEETALLESLEGKIGQPRLKPPFPAVAGLYAKPTVVNNVETLTNVPRIIEKGADWFRSFGTEKSP
GTKAVSISGHVKKPGNYEIPLGITIREFIFDWAGGMRDPNLPLKFIIPGGASSNWLTEQHLDLPMTWDDMAAAGTMLGSG
AMVVLDTSVPVVRAALKVDEFFKHESCGKCSPCREGTHFLVKVWERIDEGHGRVGDIELLADVGKQMLGKCFCPLGDSSV
SAVNSAIKFFRPELDAAIDAQH

Sequences:

>Translated_422_residues
MPKTELKEYIVMRNREIENIRDLDVYLASGGYAMAKQALTSMTPAAIIDEVKKSGLRGRGGAGFPTGVKWSFVPKELNPK
YLVVNADESEPGTFNNHEIIDENPHQLLEGIVISAFAIGANVAYIYIRGEFAYGARFLEQKIAEARARGLIGKNLFGTGY
DVEIYVHRGAGAYICGEETALLESLEGKIGQPRLKPPFPAVAGLYAKPTVVNNVETLTNVPRIIEKGADWFRSFGTEKSP
GTKAVSISGHVKKPGNYEIPLGITIREFIFDWAGGMRDPNLPLKFIIPGGASSNWLTEQHLDLPMTWDDMAAAGTMLGSG
AMVVLDTSVPVVRAALKVDEFFKHESCGKCSPCREGTHFLVKVWERIDEGHGRVGDIELLADVGKQMLGKCFCPLGDSSV
SAVNSAIKFFRPELDAAIDAQH
>Mature_421_residues
PKTELKEYIVMRNREIENIRDLDVYLASGGYAMAKQALTSMTPAAIIDEVKKSGLRGRGGAGFPTGVKWSFVPKELNPKY
LVVNADESEPGTFNNHEIIDENPHQLLEGIVISAFAIGANVAYIYIRGEFAYGARFLEQKIAEARARGLIGKNLFGTGYD
VEIYVHRGAGAYICGEETALLESLEGKIGQPRLKPPFPAVAGLYAKPTVVNNVETLTNVPRIIEKGADWFRSFGTEKSPG
TKAVSISGHVKKPGNYEIPLGITIREFIFDWAGGMRDPNLPLKFIIPGGASSNWLTEQHLDLPMTWDDMAAAGTMLGSGA
MVVLDTSVPVVRAALKVDEFFKHESCGKCSPCREGTHFLVKVWERIDEGHGRVGDIELLADVGKQMLGKCFCPLGDSSVS
AVNSAIKFFRPELDAAIDAQH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=392, Percent_Identity=48.469387755102, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI20149568, Length=392, Percent_Identity=48.469387755102, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1788620, Length=412, Percent_Identity=47.5728155339806, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17531727, Length=379, Percent_Identity=47.2295514511873, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI19920794, Length=372, Percent_Identity=49.4623655913978, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI45550452, Length=375, Percent_Identity=45.6, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24653819, Length=382, Percent_Identity=35.0785340314136, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=32.6760563380282, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46032; Mature: 45901

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTELKEYIVMRNREIENIRDLDVYLASGGYAMAKQALTSMTPAAIIDEVKKSGLRGRG
CCHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
GAGFPTGVKWSFVPKELNPKYLVVNADESEPGTFNNHEIIDENPHQLLEGIVISAFAIGA
CCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
NVAYIYIRGEFAYGARFLEQKIAEARARGLIGKNLFGTGYDVEIYVHRGAGAYICGEETA
CEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEECCHHH
LLESLEGKIGQPRLKPPFPAVAGLYAKPTVVNNVETLTNVPRIIEKGADWFRSFGTEKSP
HHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GTKAVSISGHVKKPGNYEIPLGITIREFIFDWAGGMRDPNLPLKFIIPGGASSNWLTEQH
CCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHC
LDLPMTWDDMAAAGTMLGSGAMVVLDTSVPVVRAALKVDEFFKHESCGKCSPCREGTHFL
CCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VKVWERIDEGHGRVGDIELLADVGKQMLGKCFCPLGDSSVSAVNSAIKFFRPELDAAIDA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCC
QH
CC
>Mature Secondary Structure 
PKTELKEYIVMRNREIENIRDLDVYLASGGYAMAKQALTSMTPAAIIDEVKKSGLRGRG
CHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
GAGFPTGVKWSFVPKELNPKYLVVNADESEPGTFNNHEIIDENPHQLLEGIVISAFAIGA
CCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
NVAYIYIRGEFAYGARFLEQKIAEARARGLIGKNLFGTGYDVEIYVHRGAGAYICGEETA
CEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEECCHHH
LLESLEGKIGQPRLKPPFPAVAGLYAKPTVVNNVETLTNVPRIIEKGADWFRSFGTEKSP
HHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GTKAVSISGHVKKPGNYEIPLGITIREFIFDWAGGMRDPNLPLKFIIPGGASSNWLTEQH
CCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHC
LDLPMTWDDMAAAGTMLGSGAMVVLDTSVPVVRAALKVDEFFKHESCGKCSPCREGTHFL
CCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VKVWERIDEGHGRVGDIELLADVGKQMLGKCFCPLGDSSVSAVNSAIKFFRPELDAAIDA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCC
QH
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]