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The map label for this gene is greA [H]

Identifier: 159898200

GI number: 159898200

Start: 1942562

End: 1943029

Strand: Reverse

Name: greA [H]

Synonym: Haur_1676

Alternate gene names: 159898200

Gene position: 1943029-1942562 (Counterclockwise)

Preceding gene: 159898204

Following gene: 159898199

Centisome position: 30.62

GC content: 49.57

Gene sequence:

>468_bases
ATGACCGATAAGCCGTTTTATCTGACTAAAGAGGGTCGTGCGAAACTGGAAGCGGAAATTGAGGAACTTGAAACAACTGG
CCGTATGGAAGTTGCCGAGCGGATTCGTCAAGCCAAAGAACTCGGCGATATTTCCGAAAGCGGCGAGTACGAAGATGCCA
AAAATAAGCAGGCTTTCCTCGAAGGGCGCATCCGCGAAATCCGTAACATTTTAAATAACGCCCAAATGATTGAAGAAGAT
GGGGATAACCGCGAGGTACGGCTAGGGTCATCAGTCACGATTCGCTATGAAGACGGCGAAGAAGAAGAATGGATGATTGT
CGGTGCTGCCGAAGCTAGCCCCCGCGATGGCAAAATCTCCAATGAATCGCCGATTGGCAGCGCTTTGATGGGTAAACGCG
CTCGTCAAAAAGCCACTGCCAGCACCCCCAGCGGGATTATGAAAATGACAGTTCTCAAAATTCGCTAA

Upstream 100 bases:

>100_bases
CCGTATAATGGCTAACACGTTCACGTGAGCAATCGCGTGGGCGTTTCTTTTGACTGAGAGTCCTCTCGGTTTTTTTCATT
CCATAATCGAGGCAAGTACT

Downstream 100 bases:

>100_bases
CCAATCCCCACTCAATTCAGTGTGTGACAAAGCCTAAGGCGCACTGGAGAGTGCGCCTTTTGGATTATACAGCGCGTTCT
CGGCTATAATAGCCAGCAGG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MTDKPFYLTKEGRAKLEAEIEELETTGRMEVAERIRQAKELGDISESGEYEDAKNKQAFLEGRIREIRNILNNAQMIEED
GDNREVRLGSSVTIRYEDGEEEEWMIVGAAEASPRDGKISNESPIGSALMGKRARQKATASTPSGIMKMTVLKIR

Sequences:

>Translated_155_residues
MTDKPFYLTKEGRAKLEAEIEELETTGRMEVAERIRQAKELGDISESGEYEDAKNKQAFLEGRIREIRNILNNAQMIEED
GDNREVRLGSSVTIRYEDGEEEEWMIVGAAEASPRDGKISNESPIGSALMGKRARQKATASTPSGIMKMTVLKIR
>Mature_154_residues
TDKPFYLTKEGRAKLEAEIEELETTGRMEVAERIRQAKELGDISESGEYEDAKNKQAFLEGRIREIRNILNNAQMIEEDG
DNREVRLGSSVTIRYEDGEEEEWMIVGAAEASPRDGKISNESPIGSALMGKRARQKATASTPSGIMKMTVLKIR

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=150, Percent_Identity=36.6666666666667, Blast_Score=87, Evalue=4e-19,
Organism=Escherichia coli, GI87082261, Length=139, Percent_Identity=31.6546762589928, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17395; Mature: 17264

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKPFYLTKEGRAKLEAEIEELETTGRMEVAERIRQAKELGDISESGEYEDAKNKQAFL
CCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
EGRIREIRNILNNAQMIEEDGDNREVRLGSSVTIRYEDGEEEEWMIVGAAEASPRDGKIS
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCEEEEECCCCCCCCCCCC
NESPIGSALMGKRARQKATASTPSGIMKMTVLKIR
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TDKPFYLTKEGRAKLEAEIEELETTGRMEVAERIRQAKELGDISESGEYEDAKNKQAFL
CCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
EGRIREIRNILNNAQMIEEDGDNREVRLGSSVTIRYEDGEEEEWMIVGAAEASPRDGKIS
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCEEEEECCCCCCCCCCCC
NESPIGSALMGKRARQKATASTPSGIMKMTVLKIR
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA