Click here to switch to the map view.

The map label for this gene is lysS

Identifier: 159898199

GI number: 159898199

Start: 1940893

End: 1942371

Strand: Reverse

Name: lysS

Synonym: Haur_1675

Alternate gene names: 159898199

Gene position: 1942371-1940893 (Counterclockwise)

Preceding gene: 159898200

Following gene: 159898198

Centisome position: 30.6

GC content: 50.71

Gene sequence:

>1479_bases
ATGGAATTAAACGATTTACAGCAAACACGCTATGGCAAGCTACAGGCGCTGCAAGCCGCTGGCATCGAGCCATATCCAGC
CCGAGTGCCGCAACGCACTCATACATTAACCGCCGTGCGTGAGCAATTTTCGGCCCTGGTTGAGGCCAATGCCACGGTAA
CAATTATGGGGCGCTTGCGCCAACGTCGCGTTATGGGCAAATCAGCGTTCGCCCATTTAAATGATGATCATGGCGCGTTT
CAAATTTTCCTCAGCAAAGCCGATGTTGGCGATGAGCCATTCAAGCATTTTGTTGATCTGACTGATCTTGGCGATATTAT
TGCGGTCACAGGCACGCTCTTTACGACCAAAATGGGCGAACCAAGCGTACATGTCACCAGCTGGACGATGCTCAGCAAGG
CGATCACGCCGCCACCCGACAAACGCGAAGGTCAATTTAGCGACCAAGAAGCTCGCCAACGCCAACGCTATGTTGACTTA
TCCGCCAATCCTGAAGTTCGCGAAATCTTCCGGATTCGCGCTCGTTTGATCACGGCAATGCGGCGCTACCTCGATGAACG
CGGCTTTTTGGAAGTTGAAACGCCAGTATTGCAGGGGATTTATGGTGGCGCAGCGGCGCGACCATTCACCACCCATCATA
ATCAATTGCACCAAGATTTATACCTGCGGATCGCCACCGAGCTCTATTTGAAGCGCTTGATCGTTGGCGGCTTCGATGGT
GTGTATGAAATTGGCAAAAACTTCCGCAACGAAGGCGTTGATCGCACCCATAACCCCGAATTTACCATGATCGAGGTCTA
TCAAGCCTACGGCGATTATGAATCGATTATGCAATTAACCGAGGGCATGATTCGCTTCGCTGCTGAGCAAATTTTTAACA
GCACCAGCATCGAATACCAAGGGCATCAGATCGAGCTTGGCGGTTCGTGGCAGCGCTTGACCATGCGCGATGCCATTTTT
GAAAAAACCGGGGTTGATATTCGCGAGTGCCGCGAATTTGATACACTATGGGAAGCAATTGGCGAAGCTGGCCTGAAAAT
TGAGCGCAAGCCAACCTGGGCCAAGCAAGTTGATGAGCTATTTAGTGAGTTTGTTGAGCCTGAGTTGATTCAGCCAACCT
TTATCACCGAATACCCTCAGCCACTTTCGCCTTTGGCCAAGCGCAAAGCCGATGATCCACAGTTTGTCGAGCGCTTTGAG
CTATTTATGCTTGGAGCCGAAATTGCCAACGCCTTCAGCGAATTAAACGATCCCTTCGATCAAGAGCAACGCTTCTTGGA
GCAAGGCCGCGATTATGCTGCTGGCGATGACGAAGCCATGCAAATGGACGAAGATTACCTTGAGGCGCTTAAAGTTGGTA
TGCCACCAACTGGCGGTTTAGGCATCGGGATCGATCGGCTATGTCTGTTATTTACCAATCAAACTACGATTCGTGAAGTA
ATCTTCTTCCCGCATTTGCGCAAGCAGGGCGAGGAGTAG

Upstream 100 bases:

>100_bases
AGCCAGCAGGATTCAAGCAGTAGGCCATCCAGCATAGCCCTTTTTCGTTAGCATTAGCACGGGTTGCGCTGGCTCGCTTT
ACTCTACTATTGAGATTAGC

Downstream 100 bases:

>100_bases
CAACGAGGGGCTAGGGACTAGGGGTCAGGGTTCAGAAAATTAGACCCATTAGACCAATACCTGTATCGTCAGATAGATAC
GAACCAATTGTTCAGAGGCT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MELNDLQQTRYGKLQALQAAGIEPYPARVPQRTHTLTAVREQFSALVEANATVTIMGRLRQRRVMGKSAFAHLNDDHGAF
QIFLSKADVGDEPFKHFVDLTDLGDIIAVTGTLFTTKMGEPSVHVTSWTMLSKAITPPPDKREGQFSDQEARQRQRYVDL
SANPEVREIFRIRARLITAMRRYLDERGFLEVETPVLQGIYGGAAARPFTTHHNQLHQDLYLRIATELYLKRLIVGGFDG
VYEIGKNFRNEGVDRTHNPEFTMIEVYQAYGDYESIMQLTEGMIRFAAEQIFNSTSIEYQGHQIELGGSWQRLTMRDAIF
EKTGVDIRECREFDTLWEAIGEAGLKIERKPTWAKQVDELFSEFVEPELIQPTFITEYPQPLSPLAKRKADDPQFVERFE
LFMLGAEIANAFSELNDPFDQEQRFLEQGRDYAAGDDEAMQMDEDYLEALKVGMPPTGGLGIGIDRLCLLFTNQTTIREV
IFFPHLRKQGEE

Sequences:

>Translated_492_residues
MELNDLQQTRYGKLQALQAAGIEPYPARVPQRTHTLTAVREQFSALVEANATVTIMGRLRQRRVMGKSAFAHLNDDHGAF
QIFLSKADVGDEPFKHFVDLTDLGDIIAVTGTLFTTKMGEPSVHVTSWTMLSKAITPPPDKREGQFSDQEARQRQRYVDL
SANPEVREIFRIRARLITAMRRYLDERGFLEVETPVLQGIYGGAAARPFTTHHNQLHQDLYLRIATELYLKRLIVGGFDG
VYEIGKNFRNEGVDRTHNPEFTMIEVYQAYGDYESIMQLTEGMIRFAAEQIFNSTSIEYQGHQIELGGSWQRLTMRDAIF
EKTGVDIRECREFDTLWEAIGEAGLKIERKPTWAKQVDELFSEFVEPELIQPTFITEYPQPLSPLAKRKADDPQFVERFE
LFMLGAEIANAFSELNDPFDQEQRFLEQGRDYAAGDDEAMQMDEDYLEALKVGMPPTGGLGIGIDRLCLLFTNQTTIREV
IFFPHLRKQGEE
>Mature_492_residues
MELNDLQQTRYGKLQALQAAGIEPYPARVPQRTHTLTAVREQFSALVEANATVTIMGRLRQRRVMGKSAFAHLNDDHGAF
QIFLSKADVGDEPFKHFVDLTDLGDIIAVTGTLFTTKMGEPSVHVTSWTMLSKAITPPPDKREGQFSDQEARQRQRYVDL
SANPEVREIFRIRARLITAMRRYLDERGFLEVETPVLQGIYGGAAARPFTTHHNQLHQDLYLRIATELYLKRLIVGGFDG
VYEIGKNFRNEGVDRTHNPEFTMIEVYQAYGDYESIMQLTEGMIRFAAEQIFNSTSIEYQGHQIELGGSWQRLTMRDAIF
EKTGVDIRECREFDTLWEAIGEAGLKIERKPTWAKQVDELFSEFVEPELIQPTFITEYPQPLSPLAKRKADDPQFVERFE
LFMLGAEIANAFSELNDPFDQEQRFLEQGRDYAAGDDEAMQMDEDYLEALKVGMPPTGGLGIGIDRLCLLFTNQTTIREV
IFFPHLRKQGEE

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=506, Percent_Identity=39.7233201581028, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI194272210, Length=509, Percent_Identity=39.4891944990177, Blast_Score=348, Evalue=9e-96,
Organism=Homo sapiens, GI45439306, Length=504, Percent_Identity=22.2222222222222, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI40789249, Length=245, Percent_Identity=24.8979591836735, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790571, Length=495, Percent_Identity=47.0707070707071, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1789256, Length=498, Percent_Identity=47.9919678714859, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI87082379, Length=319, Percent_Identity=32.6018808777429, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788173, Length=250, Percent_Identity=28.8, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=502, Percent_Identity=40.8366533864542, Blast_Score=357, Evalue=7e-99,
Organism=Caenorhabditis elegans, GI17535927, Length=502, Percent_Identity=40.8366533864542, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI71994340, Length=502, Percent_Identity=40.8366533864542, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI32566633, Length=255, Percent_Identity=28.2352941176471, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=339, Percent_Identity=23.0088495575221, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=508, Percent_Identity=40.5511811023622, Blast_Score=333, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6324256, Length=488, Percent_Identity=30.9426229508197, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6323011, Length=320, Percent_Identity=24.375, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640849, Length=508, Percent_Identity=39.1732283464567, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24640851, Length=508, Percent_Identity=39.1732283464567, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24584738, Length=325, Percent_Identity=26.4615384615385, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_HERA2 (A9B5T5)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001544446.1
- ProteinModelPortal:   A9B5T5
- SMR:   A9B5T5
- GeneID:   5733559
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_1675
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- ProtClustDB:   PRK00484
- BioCyc:   HAUR316274:HAUR_1675-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56253; Mature: 56253

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNDLQQTRYGKLQALQAAGIEPYPARVPQRTHTLTAVREQFSALVEANATVTIMGRLR
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
QRRVMGKSAFAHLNDDHGAFQIFLSKADVGDEPFKHFVDLTDLGDIIAVTGTLFTTKMGE
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHCHHHCCHHHHHHHHHHEECCCC
PSVHVTSWTMLSKAITPPPDKREGQFSDQEARQRQRYVDLSANPEVREIFRIRARLITAM
CCEEEEHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHH
RRYLDERGFLEVETPVLQGIYGGAAARPFTTHHNQLHQDLYLRIATELYLKRLIVGGFDG
HHHHHHCCCEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VYEIGKNFRNEGVDRTHNPEFTMIEVYQAYGDYESIMQLTEGMIRFAAEQIFNSTSIEYQ
HHHHHHHHHHCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GHQIELGGSWQRLTMRDAIFEKTGVDIRECREFDTLWEAIGEAGLKIERKPTWAKQVDEL
CCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHH
FSEFVEPELIQPTFITEYPQPLSPLAKRKADDPQFVERFELFMLGAEIANAFSELNDPFD
HHHHCCCCCCCCCHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QEQRFLEQGRDYAAGDDEAMQMDEDYLEALKVGMPPTGGLGIGIDRLCLLFTNQTTIREV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
IFFPHLRKQGEE
HHHHHHHHCCCC
>Mature Secondary Structure
MELNDLQQTRYGKLQALQAAGIEPYPARVPQRTHTLTAVREQFSALVEANATVTIMGRLR
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
QRRVMGKSAFAHLNDDHGAFQIFLSKADVGDEPFKHFVDLTDLGDIIAVTGTLFTTKMGE
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHCHHHCCHHHHHHHHHHEECCCC
PSVHVTSWTMLSKAITPPPDKREGQFSDQEARQRQRYVDLSANPEVREIFRIRARLITAM
CCEEEEHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHH
RRYLDERGFLEVETPVLQGIYGGAAARPFTTHHNQLHQDLYLRIATELYLKRLIVGGFDG
HHHHHHCCCEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VYEIGKNFRNEGVDRTHNPEFTMIEVYQAYGDYESIMQLTEGMIRFAAEQIFNSTSIEYQ
HHHHHHHHHHCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GHQIELGGSWQRLTMRDAIFEKTGVDIRECREFDTLWEAIGEAGLKIERKPTWAKQVDEL
CCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHH
FSEFVEPELIQPTFITEYPQPLSPLAKRKADDPQFVERFELFMLGAEIANAFSELNDPFD
HHHHCCCCCCCCCHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QEQRFLEQGRDYAAGDDEAMQMDEDYLEALKVGMPPTGGLGIGIDRLCLLFTNQTTIREV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
IFFPHLRKQGEE
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA