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The map label for this gene is dapE [H]

Identifier: 159898184

GI number: 159898184

Start: 1928016

End: 1929389

Strand: Reverse

Name: dapE [H]

Synonym: Haur_1660

Alternate gene names: 159898184

Gene position: 1929389-1928016 (Counterclockwise)

Preceding gene: 159898185

Following gene: 159898182

Centisome position: 30.4

GC content: 52.69

Gene sequence:

>1374_bases
ATGACGGTTGATGCTGCCTTAGCATGGGTCAACGATCGCCACGACGATCTATTAGCACGATTTAGTGAATTGCTGCGAAT
TCCTTCAGTGAGTACCGACCCCGCCTATGCTGCCGATGTGCAACGCTGCGCCGATTGGCTGGTTGGCGACCTACAACGAA
TTGGGTTTGCTAATTGCCAAGCGATTGCGACCAGCGGGCATCCTGTGGTATATGGCGAATGGCTCAAGGCTGGGTCGGCG
GCTCCAACTATTTTGGTCTATGCCCATTATGATGTTCAACCAGTCGAGCCGCTAGAATTGTGGAAAAACCCGCCATTCGA
GCCAGTGCTGCGCGATGGCAAATTGTATGCGCGTGGCTCGATCGACGATAAATGTGGAGCATTCGCCAATTTGATCGCCT
TTGAAGCGCTGCTTGCGACCACTGGCACACTCCCCGTCAACATTAAAGTCATCTTTGAGGGCGAAGAGGAAACCGGCTCA
CCATCAATGGAGCCATTTGTGCGCGAGTATCAAGCTTTGCTCGCCGCCGATTTGATGTTGATTTGTGATGGTGGCTCGCT
GCCCGATCAACCGTTGATGTTCTATACCGCCCGTGGCATTATTGGGGCCGAAGTCAAAGTGACTGGGCCAAGCCATGATC
TGCATTCGGGCTTAGTTGGCGGGGCAGTCCAAAACCCAATCCATGTGGTTGGCAACATTATCGCCGCGCTACACGACGAA
ACCGGACGTATCCAAATTCCTGGTTTTTATGCTGATGTTCAAACGCCTTCGGCTACAGAGCAAGCCCTGATGGATGCGCA
AGAACAAGATTTTTTCGCCATGTTGAAAAATGAAAGTGGTCGCGAGAGCTTTTGGGCCAACGAGCTTGGTTCATTGGTCG
CCCGCACCACGGCCTTGCCAACCTGCGATGTAAACGGCATTTGGGGTGGCTACCAAGGCGCAGGCACCAAAACAATCATT
CCGGCTGAGGCTGGCTTTAAGGTTACGATGCGCTTGGTTGCCAACCAAGATCCTCAGGCTGTGCTCGAATCATTCTGCCA
GTTTGTCCAAGGCTTCACCAGCTCCACCGCTGAGGTTCACGTTACCAAAGGTCAAACCAGTTACCCGGTTAATTTGCTCT
ACGATGGCCCAGTGATTGATGCATTGCAAGCGGCATTTGAAGCAACGTGGGGCAAGCCAGCCATGCTCTATCGCCAAGGT
GGCTCAGTGCCAATTATGGGCATGTTTCAGCGCGAGTTGGGCATTGATTTAGCAACACTCGGCTTTGGCACCGGCGATAA
TGGTCATGCGCCCAACGAATATCTGCTGGTTGATGCCTTCTTCCGTGGAGTCGCCACCGCCATCCATTTTTACACTCGTA
TGGGTCAGCAATAA

Upstream 100 bases:

>100_bases
GATTATTATTATTTGTTCAAACCCTAGGCATAGCCTATACTGAATCACGGGATCGATTTGGAACATACATGGTTGTGTTC
CTCAGTGTGAGGTCTATCAG

Downstream 100 bases:

>100_bases
ACGAAACAAGCGCAGAGGGATTGGATTCCCTCTGCGCCACGACCGCTAGCAATAACCAATTAGTCTTGGAAGGTTAAAAC
GGCAAACGGCTCACCAAAAG

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTVDAALAWVNDRHDDLLARFSELLRIPSVSTDPAYAADVQRCADWLVGDLQRIGFANCQAIATSGHPVVYGEWLKAGSA
APTILVYAHYDVQPVEPLELWKNPPFEPVLRDGKLYARGSIDDKCGAFANLIAFEALLATTGTLPVNIKVIFEGEEETGS
PSMEPFVREYQALLAADLMLICDGGSLPDQPLMFYTARGIIGAEVKVTGPSHDLHSGLVGGAVQNPIHVVGNIIAALHDE
TGRIQIPGFYADVQTPSATEQALMDAQEQDFFAMLKNESGRESFWANELGSLVARTTALPTCDVNGIWGGYQGAGTKTII
PAEAGFKVTMRLVANQDPQAVLESFCQFVQGFTSSTAEVHVTKGQTSYPVNLLYDGPVIDALQAAFEATWGKPAMLYRQG
GSVPIMGMFQRELGIDLATLGFGTGDNGHAPNEYLLVDAFFRGVATAIHFYTRMGQQ

Sequences:

>Translated_457_residues
MTVDAALAWVNDRHDDLLARFSELLRIPSVSTDPAYAADVQRCADWLVGDLQRIGFANCQAIATSGHPVVYGEWLKAGSA
APTILVYAHYDVQPVEPLELWKNPPFEPVLRDGKLYARGSIDDKCGAFANLIAFEALLATTGTLPVNIKVIFEGEEETGS
PSMEPFVREYQALLAADLMLICDGGSLPDQPLMFYTARGIIGAEVKVTGPSHDLHSGLVGGAVQNPIHVVGNIIAALHDE
TGRIQIPGFYADVQTPSATEQALMDAQEQDFFAMLKNESGRESFWANELGSLVARTTALPTCDVNGIWGGYQGAGTKTII
PAEAGFKVTMRLVANQDPQAVLESFCQFVQGFTSSTAEVHVTKGQTSYPVNLLYDGPVIDALQAAFEATWGKPAMLYRQG
GSVPIMGMFQRELGIDLATLGFGTGDNGHAPNEYLLVDAFFRGVATAIHFYTRMGQQ
>Mature_456_residues
TVDAALAWVNDRHDDLLARFSELLRIPSVSTDPAYAADVQRCADWLVGDLQRIGFANCQAIATSGHPVVYGEWLKAGSAA
PTILVYAHYDVQPVEPLELWKNPPFEPVLRDGKLYARGSIDDKCGAFANLIAFEALLATTGTLPVNIKVIFEGEEETGSP
SMEPFVREYQALLAADLMLICDGGSLPDQPLMFYTARGIIGAEVKVTGPSHDLHSGLVGGAVQNPIHVVGNIIAALHDET
GRIQIPGFYADVQTPSATEQALMDAQEQDFFAMLKNESGRESFWANELGSLVARTTALPTCDVNGIWGGYQGAGTKTIIP
AEAGFKVTMRLVANQDPQAVLESFCQFVQGFTSSTAEVHVTKGQTSYPVNLLYDGPVIDALQAAFEATWGKPAMLYRQGG
SVPIMGMFQRELGIDLATLGFGTGDNGHAPNEYLLVDAFFRGVATAIHFYTRMGQQ

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=467, Percent_Identity=27.8372591006424, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI21071039, Length=477, Percent_Identity=26.6247379454927, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI271398277, Length=299, Percent_Identity=29.4314381270903, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17563146, Length=473, Percent_Identity=31.0782241014799, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI193211059, Length=461, Percent_Identity=28.6334056399132, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321159, Length=457, Percent_Identity=27.5711159737418, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319758, Length=465, Percent_Identity=25.5913978494624, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24585880, Length=471, Percent_Identity=27.8131634819533, Blast_Score=175, Evalue=7e-44,
Organism=Drosophila melanogaster, GI21356353, Length=237, Percent_Identity=29.535864978903, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49323; Mature: 49192

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDAALAWVNDRHDDLLARFSELLRIPSVSTDPAYAADVQRCADWLVGDLQRIGFANCQ
CCCHHEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
AIATSGHPVVYGEWLKAGSAAPTILVYAHYDVQPVEPLELWKNPPFEPVLRDGKLYARGS
EEECCCCCEEEEHHHCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCHHHCCCEEEECCC
IDDKCGAFANLIAFEALLATTGTLPVNIKVIFEGEEETGSPSMEPFVREYQALLAADLML
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCEEE
ICDGGSLPDQPLMFYTARGIIGAEVKVTGPSHDLHSGLVGGAVQNPIHVVGNIIAALHDE
EECCCCCCCCCEEEEEECCEEECEEEEECCCCHHHHCHHCCHHCCHHHHHHHHHHHHCCC
TGRIQIPGFYADVQTPSATEQALMDAQEQDFFAMLKNESGRESFWANELGSLVARTTALP
CCEEECCCEEECCCCCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
TCDVNGIWGGYQGAGTKTIIPAEAGFKVTMRLVANQDPQAVLESFCQFVQGFTSSTAEVH
CCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEE
VTKGQTSYPVNLLYDGPVIDALQAAFEATWGKPAMLYRQGGSVPIMGMFQRELGIDLATL
EECCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEE
GFGTGDNGHAPNEYLLVDAFFRGVATAIHFYTRMGQQ
ECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVDAALAWVNDRHDDLLARFSELLRIPSVSTDPAYAADVQRCADWLVGDLQRIGFANCQ
CCHHEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
AIATSGHPVVYGEWLKAGSAAPTILVYAHYDVQPVEPLELWKNPPFEPVLRDGKLYARGS
EEECCCCCEEEEHHHCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCHHHCCCEEEECCC
IDDKCGAFANLIAFEALLATTGTLPVNIKVIFEGEEETGSPSMEPFVREYQALLAADLML
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCEEE
ICDGGSLPDQPLMFYTARGIIGAEVKVTGPSHDLHSGLVGGAVQNPIHVVGNIIAALHDE
EECCCCCCCCCEEEEEECCEEECEEEEECCCCHHHHCHHCCHHCCHHHHHHHHHHHHCCC
TGRIQIPGFYADVQTPSATEQALMDAQEQDFFAMLKNESGRESFWANELGSLVARTTALP
CCEEECCCEEECCCCCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
TCDVNGIWGGYQGAGTKTIIPAEAGFKVTMRLVANQDPQAVLESFCQFVQGFTSSTAEVH
CCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEE
VTKGQTSYPVNLLYDGPVIDALQAAFEATWGKPAMLYRQGGSVPIMGMFQRELGIDLATL
EECCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEE
GFGTGDNGHAPNEYLLVDAFFRGVATAIHFYTRMGQQ
ECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA