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The map label for this gene is purD [H]

Identifier: 159897475

GI number: 159897475

Start: 1082410

End: 1083672

Strand: Reverse

Name: purD [H]

Synonym: Haur_0946

Alternate gene names: 159897475

Gene position: 1083672-1082410 (Counterclockwise)

Preceding gene: 159897476

Following gene: 159897474

Centisome position: 17.07

GC content: 57.17

Gene sequence:

>1263_bases
ATGCGAGTATTACTTGTTGGAGCCGGCGGGCGTGAACATGCCCTAGCATGGAAAATCGCCCAATCGCCATTATGCGAACA
CCTGTGGATCGTCCCTGGTAATCCCGGCACGGCAACGCTTGGTACAAACGTCGATTTAGCCGCAGACGATGCTGCTGGCA
TTGTGGGTTTAGCCACCCGCGAACAGATAGATTTGGTGGTTGTCGGGCCAGAAGCGCCCTTGGTCGCAGGCTTAGGCGAG
GTCTGCGAGGCTGTCGATTTGGCCATGTTTGGGCCTTCGTCTGAGGCCGCCCAACTTGAGGGCAGCAAAGCCTTCGCCAA
AATGATTATGCTTGAGGCGGGCGTGCCAACCGCTGCTGCCCATACCTTCGATAACTCCGCCGCTGCAATCGCCTTTGTTG
AAGCCGATCAACAGGCTTGGGTGGTTAAAGCTGATGGTTTGGCCGCTGGCAAGGGTGTGATCGTGCCCGAAGATGGCGAT
GTTGCCGCCACAATCAGCGCCATTCGCGAATTGGCCGCGACCAATGCTGGCACGCGCTTGGTGCTTGAAGAAAAACTAAC
TGGCCCCGAAGTTTCGTTATTGGCCTTATGCGATGGCGAACGGGCGATTCCGCTGATCGCCGCTCAAGACCACAAGCGCA
TCGGCGATAACGACACTGGCGGTAATACCGGCGGCATGGGATCATATGCGCCAGCCCCAATTTTGCCCTATGCCGAAGCG
CAAGCCTTGGTTGATGTGATTTTCACGCCAGTGCTGCGCACCATGGCCGCTCGCCAAATTCCCTATCGTGGGGTGTTATA
TGCTGGTTTGATGCTCACCCCCAACGGCGTAAAAGTCATCGAATTTAATGCCCGCTTTGGCGACCCCGAAACCCAAGCCT
TGCTGCCCTTGCTCAAAAGCGATTTGCTCGAACTGCTGCACAAGGTTGCCACCAGCAATTTGGCCGATGTCACAATCGAA
TGGCACGACGGCGCGGCGGTCTGTGTGGTGCTTTCGGCGGCAGGCTATCCCGCCAAACCACGCACCGGCGACGTGATCAC
TGGTTTAGATCGTTTGCCCAGCGATGTGCTAGCGTTTCAAGCGGGCACGGCCACCAATGCTCAAGGTGAGTTGGTAACCG
CAGGCGGGCGGGTTTTGGGCATCACCGCAGTTGCGCCAACATTGCGCGAAGCCCGCGATAAAGCCTACGCCGCAACCGAA
TTGGTCGAATTTGCTGGCAAATATCAACGCAGCGATATCGCCGCACGGGGGTTAGCAGAATGA

Upstream 100 bases:

>100_bases
AAAGCCTCGAACAGCGTGTTTAGGCCTGATCCGCAACTGGCTGTTCTCGGATTATAATAGGGCTTGAACCCGTTACGAGC
CTATTGAGGATAGCAACGCA

Downstream 100 bases:

>100_bases
TGCGGCTTGCCGTGATGGTATCGGGCAGTGGCTCCAATTTACAAGCCTTGATCGATGCTCAAAAATCGCACCAACTCAAC
GCCACAATCAAGGTGGTGAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRVLLVGAGGREHALAWKIAQSPLCEHLWIVPGNPGTATLGTNVDLAADDAAGIVGLATREQIDLVVVGPEAPLVAGLGE
VCEAVDLAMFGPSSEAAQLEGSKAFAKMIMLEAGVPTAAAHTFDNSAAAIAFVEADQQAWVVKADGLAAGKGVIVPEDGD
VAATISAIRELAATNAGTRLVLEEKLTGPEVSLLALCDGERAIPLIAAQDHKRIGDNDTGGNTGGMGSYAPAPILPYAEA
QALVDVIFTPVLRTMAARQIPYRGVLYAGLMLTPNGVKVIEFNARFGDPETQALLPLLKSDLLELLHKVATSNLADVTIE
WHDGAAVCVVLSAAGYPAKPRTGDVITGLDRLPSDVLAFQAGTATNAQGELVTAGGRVLGITAVAPTLREARDKAYAATE
LVEFAGKYQRSDIAARGLAE

Sequences:

>Translated_420_residues
MRVLLVGAGGREHALAWKIAQSPLCEHLWIVPGNPGTATLGTNVDLAADDAAGIVGLATREQIDLVVVGPEAPLVAGLGE
VCEAVDLAMFGPSSEAAQLEGSKAFAKMIMLEAGVPTAAAHTFDNSAAAIAFVEADQQAWVVKADGLAAGKGVIVPEDGD
VAATISAIRELAATNAGTRLVLEEKLTGPEVSLLALCDGERAIPLIAAQDHKRIGDNDTGGNTGGMGSYAPAPILPYAEA
QALVDVIFTPVLRTMAARQIPYRGVLYAGLMLTPNGVKVIEFNARFGDPETQALLPLLKSDLLELLHKVATSNLADVTIE
WHDGAAVCVVLSAAGYPAKPRTGDVITGLDRLPSDVLAFQAGTATNAQGELVTAGGRVLGITAVAPTLREARDKAYAATE
LVEFAGKYQRSDIAARGLAE
>Mature_420_residues
MRVLLVGAGGREHALAWKIAQSPLCEHLWIVPGNPGTATLGTNVDLAADDAAGIVGLATREQIDLVVVGPEAPLVAGLGE
VCEAVDLAMFGPSSEAAQLEGSKAFAKMIMLEAGVPTAAAHTFDNSAAAIAFVEADQQAWVVKADGLAAGKGVIVPEDGD
VAATISAIRELAATNAGTRLVLEEKLTGPEVSLLALCDGERAIPLIAAQDHKRIGDNDTGGNTGGMGSYAPAPILPYAEA
QALVDVIFTPVLRTMAARQIPYRGVLYAGLMLTPNGVKVIEFNARFGDPETQALLPLLKSDLLELLHKVATSNLADVTIE
WHDGAAVCVVLSAAGYPAKPRTGDVITGLDRLPSDVLAFQAGTATNAQGELVTAGGRVLGITAVAPTLREARDKAYAATE
LVEFAGKYQRSDIAARGLAE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=43.0588235294118, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=43.0588235294118, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=43.0588235294118, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=43.0588235294118, Blast_Score=306, Evalue=3e-83,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=45.9207459207459, Blast_Score=342, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=39.667458432304, Blast_Score=283, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6321203, Length=441, Percent_Identity=42.6303854875283, Blast_Score=312, Evalue=7e-86,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=42.5581395348837, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=42.5581395348837, Blast_Score=298, Evalue=7e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 43391; Mature: 43391

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLVGAGGREHALAWKIAQSPLCEHLWIVPGNPGTATLGTNVDLAADDAAGIVGLATR
CEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCEEECCCCCCEEEEEEC
EQIDLVVVGPEAPLVAGLGEVCEAVDLAMFGPSSEAAQLEGSKAFAKMIMLEAGVPTAAA
CCEEEEEECCCCCHHHHHHHHHHHHHHEEECCCCCHHHCCCHHHHHHHHHHCCCCCHHHH
HTFDNSAAAIAFVEADQQAWVVKADGLAAGKGVIVPEDGDVAATISAIRELAATNAGTRL
HCCCCCCEEEEEEECCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEE
VLEEKLTGPEVSLLALCDGERAIPLIAAQDHKRIGDNDTGGNTGGMGSYAPAPILPYAEA
EEEECCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
QALVDVIFTPVLRTMAARQIPYRGVLYAGLMLTPNGVKVIEFNARFGDPETQALLPLLKS
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHH
DLLELLHKVATSNLADVTIEWHDGAAVCVVLSAAGYPAKPRTGDVITGLDRLPSDVLAFQ
HHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHCHHHCCCHHHEEE
AGTATNAQGELVTAGGRVLGITAVAPTLREARDKAYAATELVEFAGKYQRSDIAARGLAE
CCCCCCCCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRVLLVGAGGREHALAWKIAQSPLCEHLWIVPGNPGTATLGTNVDLAADDAAGIVGLATR
CEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCEEECCCCCCEEEEEEC
EQIDLVVVGPEAPLVAGLGEVCEAVDLAMFGPSSEAAQLEGSKAFAKMIMLEAGVPTAAA
CCEEEEEECCCCCHHHHHHHHHHHHHHEEECCCCCHHHCCCHHHHHHHHHHCCCCCHHHH
HTFDNSAAAIAFVEADQQAWVVKADGLAAGKGVIVPEDGDVAATISAIRELAATNAGTRL
HCCCCCCEEEEEEECCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEE
VLEEKLTGPEVSLLALCDGERAIPLIAAQDHKRIGDNDTGGNTGGMGSYAPAPILPYAEA
EEEECCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
QALVDVIFTPVLRTMAARQIPYRGVLYAGLMLTPNGVKVIEFNARFGDPETQALLPLLKS
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHH
DLLELLHKVATSNLADVTIEWHDGAAVCVVLSAAGYPAKPRTGDVITGLDRLPSDVLAFQ
HHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHCHHHCCCHHHEEE
AGTATNAQGELVTAGGRVLGITAVAPTLREARDKAYAATELVEFAGKYQRSDIAARGLAE
CCCCCCCCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]