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The map label for this gene is malE [H]

Identifier: 159897419

GI number: 159897419

Start: 1016995

End: 1018347

Strand: Reverse

Name: malE [H]

Synonym: Haur_0890

Alternate gene names: 159897419

Gene position: 1018347-1016995 (Counterclockwise)

Preceding gene: 159897420

Following gene: 159897418

Centisome position: 16.05

GC content: 51.52

Gene sequence:

>1353_bases
ATGCACCGTAAGCCATCTCAGCCACCCCGCACAGTTCGTAGCCTTGGATTCAGTTTGATCGTTCTGCTGATCTTGGCTGC
CTGTGGTCAAGCAGCCACGCCACCAACCCAAATCAACCCAACCAGCTTGCCCACCGATACACCGCCAACCGCCGTGCCAG
CCCTCGCCAACTCGGCAGATAAGGCCTTGGTTATTTGGTCAACTGGCTCAGAGGTCGAGGCACTTTCAATTCAAGCAGCA
GCAGATATCTTTGTGCAGAAAAATCCTGGGGTCTTGATTAAAGTTCAAGCCTTGCCCTGGAGCGATGCTCATACCCAAAT
TCTTGAGGCCATCGCCGCCGATCGCGGGCCAGATCTGATGGCTGGCGGTATGTCATGGGGCATCGAGTTTGGCAAATTGG
GTGGCATGATCGATCTGCAAAAGGCCTATCCCGATGTGGTAAGCGATGTTAAGCAAAAGATGTTGCCGCAACTCCAAGAT
TCATTGTTCTTGGATACAGGCGAGGTCTATGCATTACAACTCGATGTGGCATTGATGGGGATGTTCTATCGCACTGATTT
GCTGCGCGATTTAGTCGGCATGCAACAAGCTCCCCAAAACTGGACTGAACTGACCAAAGTGCTAGAAAAAGCCCGCACGA
TCAATAAAAAAGGCTTTGCGATTGGCTGGGATAACGCTGGCTGGATCAGCTACTTTACCTTTTTATATTCAGCTGGTGGC
AGTTTATATAACGACGATTGCTCCAAAGCCACAATCAACTCGGCGGCTGGCTTACAAGCTCTGACCTACTTCCGCGATCT
CTACACCAAATATCAAATTCCCACCGATACTGCCTTGGATATTGAAGGCGGCCTCGAAAGCGGCGATTATCTAGTGAGCT
ATGCTGGCACTTGGACGGTTGCAGCACTGGAATTCAATCGGCCTGAGTTGGCGGGCAAATGGGCCTTGGCTCCGCTGCCC
AAAAGCCCCAAAGGCCAAAGTGTTGCGTTCCTTGGAGGGCGGGTCATCGGCGTGATGGAAGCTTCGCAGAACAAAGATCT
GGCTGCCGAATTTATTCGCTTTTTGTATACCGAAGAAGCGGTTGCCGCCCAATCGGCCTATGCACAAAGTGCCAGTAACT
ATTATGTGCCGCCAAGTTTGCCCTTGCTCAGCGCCGCCAAATTGCCAACCCATATCGAAGCTGGCTTCCGTGGCTTGTTG
GAGCAATCGGCAGGCCCGCCCAAGTGCGTCGGCTGGGAAGAAAGCACCACCGATGTCCAAAAATTATTGCAAGAAGTCGT
CTACAACAATGCTGATCCCCAAGATGCTTTAGATCAAGCAGCGGAGATCATGAATCGCAATTTACAACCATAG

Upstream 100 bases:

>100_bases
TATATACTTAACGATGTTTGGGAAGGGTTATCGATGCACAGCCTACCAACACCCAAACAACCTGCATCGATGCATGTATA
GGCCAAGGGAGGTCAGCGTT

Downstream 100 bases:

>100_bases
GGGAGTAATTGCGCCACTGCGGGATTTGAAGTTGTACAGGGGGAATTATGCGTCGATGGCTCTATCGCTTACATCTATGG
TTGGTTCTGCTGCTGTTGAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MHRKPSQPPRTVRSLGFSLIVLLILAACGQAATPPTQINPTSLPTDTPPTAVPALANSADKALVIWSTGSEVEALSIQAA
ADIFVQKNPGVLIKVQALPWSDAHTQILEAIAADRGPDLMAGGMSWGIEFGKLGGMIDLQKAYPDVVSDVKQKMLPQLQD
SLFLDTGEVYALQLDVALMGMFYRTDLLRDLVGMQQAPQNWTELTKVLEKARTINKKGFAIGWDNAGWISYFTFLYSAGG
SLYNDDCSKATINSAAGLQALTYFRDLYTKYQIPTDTALDIEGGLESGDYLVSYAGTWTVAALEFNRPELAGKWALAPLP
KSPKGQSVAFLGGRVIGVMEASQNKDLAAEFIRFLYTEEAVAAQSAYAQSASNYYVPPSLPLLSAAKLPTHIEAGFRGLL
EQSAGPPKCVGWEESTTDVQKLLQEVVYNNADPQDALDQAAEIMNRNLQP

Sequences:

>Translated_450_residues
MHRKPSQPPRTVRSLGFSLIVLLILAACGQAATPPTQINPTSLPTDTPPTAVPALANSADKALVIWSTGSEVEALSIQAA
ADIFVQKNPGVLIKVQALPWSDAHTQILEAIAADRGPDLMAGGMSWGIEFGKLGGMIDLQKAYPDVVSDVKQKMLPQLQD
SLFLDTGEVYALQLDVALMGMFYRTDLLRDLVGMQQAPQNWTELTKVLEKARTINKKGFAIGWDNAGWISYFTFLYSAGG
SLYNDDCSKATINSAAGLQALTYFRDLYTKYQIPTDTALDIEGGLESGDYLVSYAGTWTVAALEFNRPELAGKWALAPLP
KSPKGQSVAFLGGRVIGVMEASQNKDLAAEFIRFLYTEEAVAAQSAYAQSASNYYVPPSLPLLSAAKLPTHIEAGFRGLL
EQSAGPPKCVGWEESTTDVQKLLQEVVYNNADPQDALDQAAEIMNRNLQP
>Mature_450_residues
MHRKPSQPPRTVRSLGFSLIVLLILAACGQAATPPTQINPTSLPTDTPPTAVPALANSADKALVIWSTGSEVEALSIQAA
ADIFVQKNPGVLIKVQALPWSDAHTQILEAIAADRGPDLMAGGMSWGIEFGKLGGMIDLQKAYPDVVSDVKQKMLPQLQD
SLFLDTGEVYALQLDVALMGMFYRTDLLRDLVGMQQAPQNWTELTKVLEKARTINKKGFAIGWDNAGWISYFTFLYSAGG
SLYNDDCSKATINSAAGLQALTYFRDLYTKYQIPTDTALDIEGGLESGDYLVSYAGTWTVAALEFNRPELAGKWALAPLP
KSPKGQSVAFLGGRVIGVMEASQNKDLAAEFIRFLYTEEAVAAQSAYAQSASNYYVPPSLPLLSAAKLPTHIEAGFRGLL
EQSAGPPKCVGWEESTTDVQKLLQEVVYNNADPQDALDQAAEIMNRNLQP

Specific function: Involved in an abc transport system for maltotriose [H]

COG id: COG1653

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 48667; Mature: 48667

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRKPSQPPRTVRSLGFSLIVLLILAACGQAATPPTQINPTSLPTDTPPTAVPALANSAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
KALVIWSTGSEVEALSIQAAADIFVQKNPGVLIKVQALPWSDAHTQILEAIAADRGPDLM
CEEEEEECCCCCEEEEHEEHEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEE
AGGMSWGIEFGKLGGMIDLQKAYPDVVSDVKQKMLPQLQDSLFLDTGEVYALQLDVALMG
ECCHHHCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHHHHH
MFYRTDLLRDLVGMQQAPQNWTELTKVLEKARTINKKGFAIGWDNAGWISYFTFLYSAGG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCC
SLYNDDCSKATINSAAGLQALTYFRDLYTKYQIPTDTALDIEGGLESGDYLVSYAGTWTV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEECCCEEE
AALEFNRPELAGKWALAPLPKSPKGQSVAFLGGRVIGVMEASQNKDLAAEFIRFLYTEEA
EEEECCCCCCCCCEEECCCCCCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHH
VAAQSAYAQSASNYYVPPSLPLLSAAKLPTHIEAGFRGLLEQSAGPPKCVGWEESTTDVQ
HHHHHHHHHCCCCEECCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KLLQEVVYNNADPQDALDQAAEIMNRNLQP
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHRKPSQPPRTVRSLGFSLIVLLILAACGQAATPPTQINPTSLPTDTPPTAVPALANSAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
KALVIWSTGSEVEALSIQAAADIFVQKNPGVLIKVQALPWSDAHTQILEAIAADRGPDLM
CEEEEEECCCCCEEEEHEEHEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEE
AGGMSWGIEFGKLGGMIDLQKAYPDVVSDVKQKMLPQLQDSLFLDTGEVYALQLDVALMG
ECCHHHCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHHHHH
MFYRTDLLRDLVGMQQAPQNWTELTKVLEKARTINKKGFAIGWDNAGWISYFTFLYSAGG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCC
SLYNDDCSKATINSAAGLQALTYFRDLYTKYQIPTDTALDIEGGLESGDYLVSYAGTWTV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEECCCEEE
AALEFNRPELAGKWALAPLPKSPKGQSVAFLGGRVIGVMEASQNKDLAAEFIRFLYTEEA
EEEECCCCCCCCCEEECCCCCCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHH
VAAQSAYAQSASNYYVPPSLPLLSAAKLPTHIEAGFRGLLEQSAGPPKCVGWEESTTDVQ
HHHHHHHHHCCCCEECCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KLLQEVVYNNADPQDALDQAAEIMNRNLQP
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]