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The map label for this gene is rlmL [H]

Identifier: 157963445

GI number: 157963445

Start: 4422365

End: 4423417

Strand: Direct

Name: rlmL [H]

Synonym: Spea_3633

Alternate gene names: 157963445

Gene position: 4422365-4423417 (Clockwise)

Preceding gene: 157963443

Following gene: 157963446

Centisome position: 85.46

GC content: 48.24

Gene sequence:

>1053_bases
ATGAGCCCAATATTTATGTCGATCACTACATCGGACCCAGCTGCTTTATCTCTTCCTCAAGCATTGGCCCAAGCCATAGC
CAAGCGTGCAGATTTCTTACAACAGGCTACTGCGCAGCAAACGGACTGTTACCGACTATTTCACGGTACCGTTGAGGGCT
GTAATGGACTCAATATTGATCGCTACGGCAACGCATGGCTCATTCAAAGCTTCCATCAGTCACTAACTGATGATGAGTTA
ACTGAGGTGACCGAGCAGCTGACTCAACTCGCCGAACTCGCCGTGATCTATAACGATCGTTCAGATAAAAACTCTCGAGT
GTTAAACCGATTATCAGAGGAGCGACAAGCCTTTGCCGAGAGCGAGCAGCAGATGCAAGAAAACGGCATCCAATTTACCA
GTAAGCTGCGTCATGAAGGCCAAGATCCTCTACTATTTCTCGATATGCGTATCGGTCGAGAATACGTAGCAGCAAATAGC
CAAGGCAAGAGTGTATTAAACCTATTCTCCTATACCTGCGGTATTGGCACCGCTGCGGCCGTTGGCGGTGCGACACGCGT
GGTTAACGTCGACTTTTCCTCGTTCGCTCTGGCTGCTGGACGTAAAAATGCCGCACTAAACCAAGCTGAAGATGTGTGTG
AGTTTATTCAAAGTGACGCTTTCCCTGCCTTAAGACAACTTGCCGGACTCAAAGTTGGCGGACGTCGCAATCAAAAACTG
CCAGCCTATCCAAAACTGAGTGCGACTCAGTTTGACCTAGTTTTTCTCGACCCTCCTAGATACGCCAAGAGCCCATTTGG
CATTGTCGACCTAGTGAATGACTATCAGGGCTTGTTCAAGCCTGCGCTACTCACCACTAAAGCTGGTGGCACTATCGTGT
GCTGTAATAACGTAGCAAAAGTTGATCGCGATGAATGGTTTGCAAGCTTAGTCCGCTGCGTTGAAAAGCAAGGCCGTAAA
GTCACTGGCCACACTTGGTTAGATTGCCACGCAGATTTTCCTTCATTTGATGGTAACCATCCACTGAAAATCGTAGCACT
GCAACTGAGTTAA

Upstream 100 bases:

>100_bases
CTCCCCTAGCCCTATTCTCATCGCCTTATCATCAAGATTGAGGGTATTTATTTGTGCAATTTTGCTATCATTAGCGCCGT
CACCTCTGACAGTATTTCAA

Downstream 100 bases:

>100_bases
CCCCGTTCGATTACCTGATAGAAGATAGCGACTCATTGATGACAACCTCTGTTGATGATAGCTATCCATTCCACTGAAAA
TCGTGGCGCTGCAGCTTAGT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSPIFMSITTSDPAALSLPQALAQAIAKRADFLQQATAQQTDCYRLFHGTVEGCNGLNIDRYGNAWLIQSFHQSLTDDEL
TEVTEQLTQLAELAVIYNDRSDKNSRVLNRLSEERQAFAESEQQMQENGIQFTSKLRHEGQDPLLFLDMRIGREYVAANS
QGKSVLNLFSYTCGIGTAAAVGGATRVVNVDFSSFALAAGRKNAALNQAEDVCEFIQSDAFPALRQLAGLKVGGRRNQKL
PAYPKLSATQFDLVFLDPPRYAKSPFGIVDLVNDYQGLFKPALLTTKAGGTIVCCNNVAKVDRDEWFASLVRCVEKQGRK
VTGHTWLDCHADFPSFDGNHPLKIVALQLS

Sequences:

>Translated_350_residues
MSPIFMSITTSDPAALSLPQALAQAIAKRADFLQQATAQQTDCYRLFHGTVEGCNGLNIDRYGNAWLIQSFHQSLTDDEL
TEVTEQLTQLAELAVIYNDRSDKNSRVLNRLSEERQAFAESEQQMQENGIQFTSKLRHEGQDPLLFLDMRIGREYVAANS
QGKSVLNLFSYTCGIGTAAAVGGATRVVNVDFSSFALAAGRKNAALNQAEDVCEFIQSDAFPALRQLAGLKVGGRRNQKL
PAYPKLSATQFDLVFLDPPRYAKSPFGIVDLVNDYQGLFKPALLTTKAGGTIVCCNNVAKVDRDEWFASLVRCVEKQGRK
VTGHTWLDCHADFPSFDGNHPLKIVALQLS
>Mature_349_residues
SPIFMSITTSDPAALSLPQALAQAIAKRADFLQQATAQQTDCYRLFHGTVEGCNGLNIDRYGNAWLIQSFHQSLTDDELT
EVTEQLTQLAELAVIYNDRSDKNSRVLNRLSEERQAFAESEQQMQENGIQFTSKLRHEGQDPLLFLDMRIGREYVAANSQ
GKSVLNLFSYTCGIGTAAAVGGATRVVNVDFSSFALAAGRKNAALNQAEDVCEFIQSDAFPALRQLAGLKVGGRRNQKLP
AYPKLSATQFDLVFLDPPRYAKSPFGIVDLVNDYQGLFKPALLTTKAGGTIVCCNNVAKVDRDEWFASLVRCVEKQGRKV
TGHTWLDCHADFPSFDGNHPLKIVALQLS

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=287, Percent_Identity=27.1777003484321, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787181, Length=278, Percent_Identity=26.978417266187, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 38547; Mature: 38416

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIFMSITTSDPAALSLPQALAQAIAKRADFLQQATAQQTDCYRLFHGTVEGCNGLNID
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RYGNAWLIQSFHQSLTDDELTEVTEQLTQLAELAVIYNDRSDKNSRVLNRLSEERQAFAE
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
SEQQMQENGIQFTSKLRHEGQDPLLFLDMRIGREYVAANSQGKSVLNLFSYTCGIGTAAA
HHHHHHHCCHHHHHHHHHCCCCCEEEEEEHHCHHHHHCCCCCHHHHHHHHHHHCCCHHHH
VGGATRVVNVDFSSFALAAGRKNAALNQAEDVCEFIQSDAFPALRQLAGLKVGGRRNQKL
CCCCEEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
PAYPKLSATQFDLVFLDPPRYAKSPFGIVDLVNDYQGLFKPALLTTKAGGTIVCCNNVAK
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCEEEEECCCHH
VDRDEWFASLVRCVEKQGRKVTGHTWLDCHADFPSFDGNHPLKIVALQLS
CCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure 
SPIFMSITTSDPAALSLPQALAQAIAKRADFLQQATAQQTDCYRLFHGTVEGCNGLNID
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RYGNAWLIQSFHQSLTDDELTEVTEQLTQLAELAVIYNDRSDKNSRVLNRLSEERQAFAE
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
SEQQMQENGIQFTSKLRHEGQDPLLFLDMRIGREYVAANSQGKSVLNLFSYTCGIGTAAA
HHHHHHHCCHHHHHHHHHCCCCCEEEEEEHHCHHHHHCCCCCHHHHHHHHHHHCCCHHHH
VGGATRVVNVDFSSFALAAGRKNAALNQAEDVCEFIQSDAFPALRQLAGLKVGGRRNQKL
CCCCEEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
PAYPKLSATQFDLVFLDPPRYAKSPFGIVDLVNDYQGLFKPALLTTKAGGTIVCCNNVAK
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCEEEEECCCHH
VDRDEWFASLVRCVEKQGRKVTGHTWLDCHADFPSFDGNHPLKIVALQLS
CCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA