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The map label for this gene is pitA [C]

Identifier: 157963302

GI number: 157963302

Start: 4259670

End: 4260938

Strand: Direct

Name: pitA [C]

Synonym: Spea_3488

Alternate gene names: 157963302

Gene position: 4259670-4260938 (Clockwise)

Preceding gene: 157963301

Following gene: 157963303

Centisome position: 82.32

GC content: 46.26

Gene sequence:

>1269_bases
ATGGTTGATGTATTAGTCACCAATGGCCCGTGGCTTATTGGTATTGCGGCTCTATTTGGTTTTTTAATGGCATGGGGTAT
TGGCGCCAATGACGTAGCCAATGCAATGGGAACCTCTGTAGGTTCGAATGCGATTACAATTAAACAAGCGATCATTATCG
CTATGATCTTCGAGTTTGCAGGTGCGTATCTTGCTGGTGGTGAAGTCACCAGTACAATCCGTAACGGCATTATTGACTCT
GCTTATTTTGTCGATTCACCAGAACTTCTGGTTTACGGTATGATTTCAGCATTGCTTGCTGCAGGTATCTGGCTCGTTTT
TGCCTCTGCACTGGGCTGGCCAGTATCGACCACTCACTCTATCGTTGGCGCCATCGTTGGTTTCGCCGCAGTTGGTGTGG
GTGCAGATGCTGTAGCATGGGGCAAAGTAGGTGGAATCGTTGGTTCTTGGGTCGTCACACCGGCAATATCTGGCTTTATC
GCCTTTATGATATTCCAGAGTGTACAAAAGCTCATTTTCAATACCGACAATCCTCTAGAGAACGCTAAGCGTTACGTTCC
ATTCTATATGGCTCTTGCTGGCTTTGTTATGTCACTAGTGACTGTTAAGAAAGGCCTTAAGCATGTCGGCCTAGATTTCA
GTAATGGTGAAGCGTATGCGTTATCACTGGCACTGGCAGTATTAGTCGGTATCGGTGGTATGATTGCGATTAAGCGACTT
AAGATGGACAGCAAGGCTGACCGTCAAACACAATTTGGTAACGTTGAGAAAGTCTTCGCTATCCTAATGGTTGTGACCGC
TTGTTGTATGGCATTCGCTCACGGTTCTAACGACGTTGCTAACGCAATTGGCCCATTAGCAGCAGTAGTCTCTGTTGTTG
AAAGTGGCGGTGTGATCAGCAATAAAGCAGACCTTGTTTGGTGGATCCTTCCTCTAGGTGCTGTTGGTATCGTTTTAGGT
CTTGCTATCTTTGGTCAACGCGTAATGCAAACCATAGGTAAGAACATTACTCACCTAACGCCAAGCCGTGGTTTCGCAGC
TGAACTTGCTGCGGCATCAACCGTGGTTATTGCATCGGGCACAGGTCTACCAATCTCTACTACACAAACATTAGTGGGTG
CAGTACTAGGTGTGGGTATGGCTCGTGGTATTGCGGCAATTAACATGGGTGTTGTTCGTAACATCGTGGTTTCTTGGGTT
GTAACCCTTCCAGCGGGTGCTGGCCTTTCAATCATATTCTTCTTCATGATTAAAGGCATTTTTAACTAA

Upstream 100 bases:

>100_bases
TGAGTGGGTTGGTGACTTGGCAGATCTTGCTGAACGAGTCGGTTCTCGCTTAGAGCTAATGTTAGCTCGCGTCTAATCAA
AAGATTATCAAGGTAACAAC

Downstream 100 bases:

>100_bases
ATCCAAGCAACATCAGTATTACAAATACTGAGTAAAAATGAGGGAAGTTTGATAATTTTAGTATCAAACTTCCCTTTTGC
ATTTTATCCCTCTTTTGCCA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MVDVLVTNGPWLIGIAALFGFLMAWGIGANDVANAMGTSVGSNAITIKQAIIIAMIFEFAGAYLAGGEVTSTIRNGIIDS
AYFVDSPELLVYGMISALLAAGIWLVFASALGWPVSTTHSIVGAIVGFAAVGVGADAVAWGKVGGIVGSWVVTPAISGFI
AFMIFQSVQKLIFNTDNPLENAKRYVPFYMALAGFVMSLVTVKKGLKHVGLDFSNGEAYALSLALAVLVGIGGMIAIKRL
KMDSKADRQTQFGNVEKVFAILMVVTACCMAFAHGSNDVANAIGPLAAVVSVVESGGVISNKADLVWWILPLGAVGIVLG
LAIFGQRVMQTIGKNITHLTPSRGFAAELAAASTVVIASGTGLPISTTQTLVGAVLGVGMARGIAAINMGVVRNIVVSWV
VTLPAGAGLSIIFFFMIKGIFN

Sequences:

>Translated_422_residues
MVDVLVTNGPWLIGIAALFGFLMAWGIGANDVANAMGTSVGSNAITIKQAIIIAMIFEFAGAYLAGGEVTSTIRNGIIDS
AYFVDSPELLVYGMISALLAAGIWLVFASALGWPVSTTHSIVGAIVGFAAVGVGADAVAWGKVGGIVGSWVVTPAISGFI
AFMIFQSVQKLIFNTDNPLENAKRYVPFYMALAGFVMSLVTVKKGLKHVGLDFSNGEAYALSLALAVLVGIGGMIAIKRL
KMDSKADRQTQFGNVEKVFAILMVVTACCMAFAHGSNDVANAIGPLAAVVSVVESGGVISNKADLVWWILPLGAVGIVLG
LAIFGQRVMQTIGKNITHLTPSRGFAAELAAASTVVIASGTGLPISTTQTLVGAVLGVGMARGIAAINMGVVRNIVVSWV
VTLPAGAGLSIIFFFMIKGIFN
>Mature_422_residues
MVDVLVTNGPWLIGIAALFGFLMAWGIGANDVANAMGTSVGSNAITIKQAIIIAMIFEFAGAYLAGGEVTSTIRNGIIDS
AYFVDSPELLVYGMISALLAAGIWLVFASALGWPVSTTHSIVGAIVGFAAVGVGADAVAWGKVGGIVGSWVVTPAISGFI
AFMIFQSVQKLIFNTDNPLENAKRYVPFYMALAGFVMSLVTVKKGLKHVGLDFSNGEAYALSLALAVLVGIGGMIAIKRL
KMDSKADRQTQFGNVEKVFAILMVVTACCMAFAHGSNDVANAIGPLAAVVSVVESGGVISNKADLVWWILPLGAVGIVLG
LAIFGQRVMQTIGKNITHLTPSRGFAAELAAASTVVIASGTGLPISTTQTLVGAVLGVGMARGIAAINMGVVRNIVVSWV
VTLPAGAGLSIIFFFMIKGIFN

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=252, Percent_Identity=37.6984126984127, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI5803173, Length=181, Percent_Identity=39.2265193370166, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25146401, Length=463, Percent_Identity=33.6933045356371, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI71981576, Length=448, Percent_Identity=35.2678571428571, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17557328, Length=506, Percent_Identity=26.6798418972332, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17539280, Length=271, Percent_Identity=34.6863468634686, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI32566716, Length=194, Percent_Identity=39.1752577319588, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17536725, Length=231, Percent_Identity=30.7359307359307, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319773, Length=221, Percent_Identity=38.4615384615385, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356511, Length=240, Percent_Identity=35.4166666666667, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 43576; Mature: 43576

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDVLVTNGPWLIGIAALFGFLMAWGIGANDVANAMGTSVGSNAITIKQAIIIAMIFEFA
CEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
GAYLAGGEVTSTIRNGIIDSAYFVDSPELLVYGMISALLAAGIWLVFASALGWPVSTTHS
CCHHCCCHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IVGAIVGFAAVGVGADAVAWGKVGGIVGSWVVTPAISGFIAFMIFQSVQKLIFNTDNPLE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NAKRYVPFYMALAGFVMSLVTVKKGLKHVGLDFSNGEAYALSLALAVLVGIGGMIAIKRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
KMDSKADRQTQFGNVEKVFAILMVVTACCMAFAHGSNDVANAIGPLAAVVSVVESGGVIS
HCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
NKADLVWWILPLGAVGIVLGLAIFGQRVMQTIGKNITHLTPSRGFAAELAAASTVVIASG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCEEEEECC
TGLPISTTQTLVGAVLGVGMARGIAAINMGVVRNIVVSWVVTLPAGAGLSIIFFFMIKGI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FN
CC
>Mature Secondary Structure
MVDVLVTNGPWLIGIAALFGFLMAWGIGANDVANAMGTSVGSNAITIKQAIIIAMIFEFA
CEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
GAYLAGGEVTSTIRNGIIDSAYFVDSPELLVYGMISALLAAGIWLVFASALGWPVSTTHS
CCHHCCCHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IVGAIVGFAAVGVGADAVAWGKVGGIVGSWVVTPAISGFIAFMIFQSVQKLIFNTDNPLE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NAKRYVPFYMALAGFVMSLVTVKKGLKHVGLDFSNGEAYALSLALAVLVGIGGMIAIKRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
KMDSKADRQTQFGNVEKVFAILMVVTACCMAFAHGSNDVANAIGPLAAVVSVVESGGVIS
HCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
NKADLVWWILPLGAVGIVLGLAIFGQRVMQTIGKNITHLTPSRGFAAELAAASTVVIASG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCEEEEECC
TGLPISTTQTLVGAVLGVGMARGIAAINMGVVRNIVVSWVVTLPAGAGLSIIFFFMIKGI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]