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The map label for this gene is rsmB [H]

Identifier: 157962778

GI number: 157962778

Start: 3622860

End: 3624209

Strand: Direct

Name: rsmB [H]

Synonym: Spea_2960

Alternate gene names: 157962778

Gene position: 3622860-3624209 (Clockwise)

Preceding gene: 157962777

Following gene: 157962781

Centisome position: 70.01

GC content: 47.19

Gene sequence:

>1350_bases
ATGATAGAACCGTCATCATCTCCTCAAGATGCTTTTCAAGCAGGTACTCCCGCACAAAAACGAGCGTTAAGCTATAGCAA
CACCATACATCAGCTGTTTATCAGCGTGATGAATGAGAAGCTACCTGCAGATCGCATTGTTGGTGAATACTTTAGAATCA
ATAAGAAACATGGCTCTAAAGATCGACGCGTCATAAGAGAAAGCCTGTTTGCTCTATTTCGTTGGTGGGGCTGGCTTAAA
AAGGTTCACACCAGTGAACAACAAGATGCAACCTGGTTTGCTTGTTTAAGCCTCGTTGCTGAATTAGAAGCTCATAAATG
GCCTCAATTTAGTGAGGCGTGGCGCGAGTTTGCCAACCAGTCAGCTGAGTTTAGTCTCAGCCAAGCCAGTCAAATATTGA
CCAGTGTTAGCGACAGATGTGAGTACTTAAACCAGCAGTTTTCCCACTTAAGCTTTAATGATGCTGAGTTATTACCAGAG
TGGTTTTGGACTGTCTCTCAAGTCAATGACGCAGATAAGCAAGCTGTTATCGAAGCCATGTGTTCACGTCCGCCAATCTG
GGGCCGCGCGCAAACATTGACTCAAGAGCAGCTTATCGCGTCACTTGCAAGCAAAGATATCGTTGCTATTGCCAGTGAGT
TTTTTGATGATGCAGTGAGTCTTGGACATAAGAGCATTAACCTCAATGCTATTGATGCCTATCAGCGCGGTGAGCTTGAG
ATCCAAGATCTTGGTTCTCAAGTCATCGGTCAAATCTGTGCCCCTAAAGCGGATGAGCAGTGGTGGGATACCTGCTCAGG
CGCTGGTGGTAAAACACTGCAGCTGCGCTCTTTAATGCTAAGACAAGACTCATCCGCGTCTGGTGAGATCGTCTCATCAG
ATATTCGTCGCAAACCGTTAGAAGAATTGCTAAAGCGCGCTAAACGAGCAGGCTTTAATGGCATCACGACTGCGCCTTGG
AAAAGTGATGACTTACCCGTAAAGGCCAGCTCTTTCGATGGAGTATTAGTCGATGCCCCTTGTAGCTGTACAGGCACCTG
GCGACGTAACCCAGATATGCGCTGGCTAGATGAGGCAAGCGTTGTTACCGATAAGCACGCACTACAGTTAGATATCCTCA
CTCGTAGCGCCAAGGCAGTTAAGTCTCAAGGTCAGCTGGTCTATGCCACTTGCTCATTGTCACCTATCGAAAATGAGCAG
GTAGTGAATGCCTTTTTAGCTGAAAATAGCGAGTTTGAGTTACAAACTCTGACGCACCCGTTTACCGGCGAGCAAACACA
GCTGCTCACCATCTGGCCCCATGAGGCCGATACCGACGGCATGTTTGTCGCTAAGATGAAAAAGCGCTAA

Upstream 100 bases:

>100_bases
AGGCGTTTCTATCAAGCGCCTCTACACGCAAGTGTAATCTCTGCTACAATTGCGCCAGTTTTAATTTCCAGCCAATTTAG
GCTAGCTCAGGCACATTCTA

Downstream 100 bases:

>100_bases
TACCGGTTCTCAAACGCTTAAAGTAAAAAAACGCCATACATTGTATGGCGTTTTTGTTAGCAATTTTGCCGATAATCACT
TGGTTTAATCTAGGCCTTGT

Product: Fmu (Sun) domain-containing protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MIEPSSSPQDAFQAGTPAQKRALSYSNTIHQLFISVMNEKLPADRIVGEYFRINKKHGSKDRRVIRESLFALFRWWGWLK
KVHTSEQQDATWFACLSLVAELEAHKWPQFSEAWREFANQSAEFSLSQASQILTSVSDRCEYLNQQFSHLSFNDAELLPE
WFWTVSQVNDADKQAVIEAMCSRPPIWGRAQTLTQEQLIASLASKDIVAIASEFFDDAVSLGHKSINLNAIDAYQRGELE
IQDLGSQVIGQICAPKADEQWWDTCSGAGGKTLQLRSLMLRQDSSASGEIVSSDIRRKPLEELLKRAKRAGFNGITTAPW
KSDDLPVKASSFDGVLVDAPCSCTGTWRRNPDMRWLDEASVVTDKHALQLDILTRSAKAVKSQGQLVYATCSLSPIENEQ
VVNAFLAENSEFELQTLTHPFTGEQTQLLTIWPHEADTDGMFVAKMKKR

Sequences:

>Translated_449_residues
MIEPSSSPQDAFQAGTPAQKRALSYSNTIHQLFISVMNEKLPADRIVGEYFRINKKHGSKDRRVIRESLFALFRWWGWLK
KVHTSEQQDATWFACLSLVAELEAHKWPQFSEAWREFANQSAEFSLSQASQILTSVSDRCEYLNQQFSHLSFNDAELLPE
WFWTVSQVNDADKQAVIEAMCSRPPIWGRAQTLTQEQLIASLASKDIVAIASEFFDDAVSLGHKSINLNAIDAYQRGELE
IQDLGSQVIGQICAPKADEQWWDTCSGAGGKTLQLRSLMLRQDSSASGEIVSSDIRRKPLEELLKRAKRAGFNGITTAPW
KSDDLPVKASSFDGVLVDAPCSCTGTWRRNPDMRWLDEASVVTDKHALQLDILTRSAKAVKSQGQLVYATCSLSPIENEQ
VVNAFLAENSEFELQTLTHPFTGEQTQLLTIWPHEADTDGMFVAKMKKR
>Mature_449_residues
MIEPSSSPQDAFQAGTPAQKRALSYSNTIHQLFISVMNEKLPADRIVGEYFRINKKHGSKDRRVIRESLFALFRWWGWLK
KVHTSEQQDATWFACLSLVAELEAHKWPQFSEAWREFANQSAEFSLSQASQILTSVSDRCEYLNQQFSHLSFNDAELLPE
WFWTVSQVNDADKQAVIEAMCSRPPIWGRAQTLTQEQLIASLASKDIVAIASEFFDDAVSLGHKSINLNAIDAYQRGELE
IQDLGSQVIGQICAPKADEQWWDTCSGAGGKTLQLRSLMLRQDSSASGEIVSSDIRRKPLEELLKRAKRAGFNGITTAPW
KSDDLPVKASSFDGVLVDAPCSCTGTWRRNPDMRWLDEASVVTDKHALQLDILTRSAKAVKSQGQLVYATCSLSPIENEQ
VVNAFLAENSEFELQTLTHPFTGEQTQLLTIWPHEADTDGMFVAKMKKR

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=244, Percent_Identity=28.6885245901639, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI76150623, Length=244, Percent_Identity=28.6885245901639, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI301336155, Length=199, Percent_Identity=31.1557788944724, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI39995082, Length=199, Percent_Identity=31.1557788944724, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI32698918, Length=217, Percent_Identity=27.6497695852535, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI2367212, Length=418, Percent_Identity=25.8373205741627, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081985, Length=245, Percent_Identity=26.530612244898, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17536757, Length=326, Percent_Identity=26.3803680981595, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25143471, Length=179, Percent_Identity=29.0502793296089, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319447, Length=178, Percent_Identity=31.4606741573034, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324268, Length=341, Percent_Identity=23.4604105571848, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI22024126, Length=239, Percent_Identity=26.3598326359833, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21355201, Length=206, Percent_Identity=28.6407766990291, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 50479; Mature: 50479

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEPSSSPQDAFQAGTPAQKRALSYSNTIHQLFISVMNEKLPADRIVGEYFRINKKHGSK
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCH
DRRVIRESLFALFRWWGWLKKVHTSEQQDATWFACLSLVAELEAHKWPQFSEAWREFANQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
SAEFSLSQASQILTSVSDRCEYLNQQFSHLSFNDAELLPEWFWTVSQVNDADKQAVIEAM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
CSRPPIWGRAQTLTQEQLIASLASKDIVAIASEFFDDAVSLGHKSINLNAIDAYQRGELE
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEECCCCCCCCH
IQDLGSQVIGQICAPKADEQWWDTCSGAGGKTLQLRSLMLRQDSSASGEIVSSDIRRKPL
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EELLKRAKRAGFNGITTAPWKSDDLPVKASSFDGVLVDAPCSCTGTWRRNPDMRWLDEAS
HHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCHHCCHH
VVTDKHALQLDILTRSAKAVKSQGQLVYATCSLSPIENEQVVNAFLAENSEFELQTLTHP
HHCCCHHEEEHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCC
FTGEQTQLLTIWPHEADTDGMFVAKMKKR
CCCCCEEEEEEECCCCCCCCEEEEECCCC
>Mature Secondary Structure
MIEPSSSPQDAFQAGTPAQKRALSYSNTIHQLFISVMNEKLPADRIVGEYFRINKKHGSK
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCH
DRRVIRESLFALFRWWGWLKKVHTSEQQDATWFACLSLVAELEAHKWPQFSEAWREFANQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
SAEFSLSQASQILTSVSDRCEYLNQQFSHLSFNDAELLPEWFWTVSQVNDADKQAVIEAM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
CSRPPIWGRAQTLTQEQLIASLASKDIVAIASEFFDDAVSLGHKSINLNAIDAYQRGELE
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEECCCCCCCCH
IQDLGSQVIGQICAPKADEQWWDTCSGAGGKTLQLRSLMLRQDSSASGEIVSSDIRRKPL
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EELLKRAKRAGFNGITTAPWKSDDLPVKASSFDGVLVDAPCSCTGTWRRNPDMRWLDEAS
HHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCHHCCHH
VVTDKHALQLDILTRSAKAVKSQGQLVYATCSLSPIENEQVVNAFLAENSEFELQTLTHP
HHCCCHHEEEHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCC
FTGEQTQLLTIWPHEADTDGMFVAKMKKR
CCCCCEEEEEEECCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA