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The map label for this gene is hyg [H]

Identifier: 157962625

GI number: 157962625

Start: 3424616

End: 3425653

Strand: Direct

Name: hyg [H]

Synonym: Spea_2805

Alternate gene names: 157962625

Gene position: 3424616-3425653 (Clockwise)

Preceding gene: 157962624

Following gene: 157962629

Centisome position: 66.18

GC content: 43.16

Gene sequence:

>1038_bases
ATGCCCATCTATAGAGAGTCCAGTATGAATCTGTCGTTCTTAACGTTATATCCTAGCGCACTGTGGCTACCTGAATCGCC
TGAATACGACTTGGCCTGCGACATCATAGATCATCTTGATTTTAGCTTTTGGAGACCATTGACAGAAAAGTTAGCGCTAG
AGCATCGACTCACCTATACCAAATGTACACGTGTTACAGAAGGGGCTAATCCGGTATTTATCTATGAGGGCGAACAAAAG
TTCGTATTAAAGCTGGTGCCACCAAATTGGTCTCAGCAAGCAAAATCCGAGCTCACAGGCCTCAAGCTATTGGAACAAGT
AAAACTGGCGCTAGATGTACCAAAACTTATCTGTCATGGCCACTTTCGCAACTGGGATTATATCTTCATGAGTTTTGTAG
AGGGAGAGAATCTCGCTTCCCTCTTGCCCACTCTCACGCAGCAAGATAAAGAGATGATTGCATTTCAGCTTGGTGAATTC
TGCCACCAGCTACACCAAATTCCAATTACGACTATCACAGAAACGACCACGAACGTTATCTTAGACTGGCCCATCCTTCT
GCAAGAGCAACGCGGTAACATTTATCAGAAACGGCAGCAACAAGGTTTAATTGAGCCATTTTTATCTGACCTAGAACCTT
ACTTAAACAATATTAATATCCAATGTAAGCAGGGTAGCAACCACTTCATTCATACGGATTTGCACCCAGCAAACCTGCTA
GTAAAACGCACTGCAAAAGGAGTTAGATTGACTGGAGTCATTGACTTTGGTGATGCAATTATTTGTCCCGATCCTGTATT
TGAATTTACCTCACCCGGACTGTTACTCGCGCTGTCGGATAAAAAGATATTCACTCAATTTTTAGATGGTTACGGCTTTG
ATTATAAAGATAAGCAAAGCTTGGTAGAGCAGTGTATGGCGTTATCTTTACTGCGCCATACGGGCGAACTCAACTACATA
CTCAATACTGTTCCCCATGCCAATACGGCTAAGGGGTGGAAACAAGCGGAAAGGTATCTTTTCCCACTCTGCGAATAA

Upstream 100 bases:

>100_bases
AGATTTTAACGCCCCATGCTCTAACCACCAGCGCTCAAAACACTATGATCTATAAACGCAGCTCAAGAGCTGCGTTTTTT
TACTCAGTTAGCGTTCAGTA

Downstream 100 bases:

>100_bases
CAGAGTTAAATTGCCCGGTATGAACTTGCACAACCGACAAGTTCATACTTAATATAGCGATTACTGAGCTTCGTTAGCAG
ACATAATAACATCTGCAGCC

Product: aminoglycoside phosphotransferase

Products: NA

Alternate protein names: APH(7''); Hygromycin B phosphotransferase; Hygromycin-B kinase [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MPIYRESSMNLSFLTLYPSALWLPESPEYDLACDIIDHLDFSFWRPLTEKLALEHRLTYTKCTRVTEGANPVFIYEGEQK
FVLKLVPPNWSQQAKSELTGLKLLEQVKLALDVPKLICHGHFRNWDYIFMSFVEGENLASLLPTLTQQDKEMIAFQLGEF
CHQLHQIPITTITETTTNVILDWPILLQEQRGNIYQKRQQQGLIEPFLSDLEPYLNNINIQCKQGSNHFIHTDLHPANLL
VKRTAKGVRLTGVIDFGDAIICPDPVFEFTSPGLLLALSDKKIFTQFLDGYGFDYKDKQSLVEQCMALSLLRHTGELNYI
LNTVPHANTAKGWKQAERYLFPLCE

Sequences:

>Translated_345_residues
MPIYRESSMNLSFLTLYPSALWLPESPEYDLACDIIDHLDFSFWRPLTEKLALEHRLTYTKCTRVTEGANPVFIYEGEQK
FVLKLVPPNWSQQAKSELTGLKLLEQVKLALDVPKLICHGHFRNWDYIFMSFVEGENLASLLPTLTQQDKEMIAFQLGEF
CHQLHQIPITTITETTTNVILDWPILLQEQRGNIYQKRQQQGLIEPFLSDLEPYLNNINIQCKQGSNHFIHTDLHPANLL
VKRTAKGVRLTGVIDFGDAIICPDPVFEFTSPGLLLALSDKKIFTQFLDGYGFDYKDKQSLVEQCMALSLLRHTGELNYI
LNTVPHANTAKGWKQAERYLFPLCE
>Mature_344_residues
PIYRESSMNLSFLTLYPSALWLPESPEYDLACDIIDHLDFSFWRPLTEKLALEHRLTYTKCTRVTEGANPVFIYEGEQKF
VLKLVPPNWSQQAKSELTGLKLLEQVKLALDVPKLICHGHFRNWDYIFMSFVEGENLASLLPTLTQQDKEMIAFQLGEFC
HQLHQIPITTITETTTNVILDWPILLQEQRGNIYQKRQQQGLIEPFLSDLEPYLNNINIQCKQGSNHFIHTDLHPANLLV
KRTAKGVRLTGVIDFGDAIICPDPVFEFTSPGLLLALSDKKIFTQFLDGYGFDYKDKQSLVEQCMALSLLRHTGELNYIL
NTVPHANTAKGWKQAERYLFPLCE

Specific function: The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation [H]

COG id: COG3173

COG function: function code R; Predicted aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR016259
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.119 [H]

Molecular weight: Translated: 39665; Mature: 39534

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIYRESSMNLSFLTLYPSALWLPESPEYDLACDIIDHLDFSFWRPLTEKLALEHRLTYT
CCCCCCCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
KCTRVTEGANPVFIYEGEQKFVLKLVPPNWSQQAKSELTGLKLLEQVKLALDVPKLICHG
HHHHHCCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
HFRNWDYIFMSFVEGENLASLLPTLTQQDKEMIAFQLGEFCHQLHQIPITTITETTTNVI
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEE
LDWPILLQEQRGNIYQKRQQQGLIEPFLSDLEPYLNNINIQCKQGSNHFIHTDLHPANLL
EECHHEEEHHCCCHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCHHHHH
VKRTAKGVRLTGVIDFGDAIICPDPVFEFTSPGLLLALSDKKIFTQFLDGYGFDYKDKQS
HHHCCCCEEEEEEEECCCEEECCCCCHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCHHH
LVEQCMALSLLRHTGELNYILNTVPHANTAKGWKQAERYLFPLCE
HHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PIYRESSMNLSFLTLYPSALWLPESPEYDLACDIIDHLDFSFWRPLTEKLALEHRLTYT
CCCCCCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
KCTRVTEGANPVFIYEGEQKFVLKLVPPNWSQQAKSELTGLKLLEQVKLALDVPKLICHG
HHHHHCCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
HFRNWDYIFMSFVEGENLASLLPTLTQQDKEMIAFQLGEFCHQLHQIPITTITETTTNVI
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEE
LDWPILLQEQRGNIYQKRQQQGLIEPFLSDLEPYLNNINIQCKQGSNHFIHTDLHPANLL
EECHHEEEHHCCCHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCHHHHH
VKRTAKGVRLTGVIDFGDAIICPDPVFEFTSPGLLLALSDKKIFTQFLDGYGFDYKDKQS
HHHCCCCEEEEEEEECCCEEECCCCCHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCHHH
LVEQCMALSLLRHTGELNYILNTVPHANTAKGWKQAERYLFPLCE
HHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3005976 [H]