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The map label for this gene is lpsJ [H]

Identifier: 157962624

GI number: 157962624

Start: 3423868

End: 3424524

Strand: Direct

Name: lpsJ [H]

Synonym: Spea_2804

Alternate gene names: 157962624

Gene position: 3423868-3424524 (Clockwise)

Preceding gene: 157962623

Following gene: 157962625

Centisome position: 66.17

GC content: 47.03

Gene sequence:

>657_bases
ATGGCATTATCAAGAGAACAACTGGCACAGCGTGTAGCACAAGAACTCCAAGATGGCTTTTACGTCAACCTAGGCATTGG
TATTCCAACACTCGTTGCTAACTATATCCCTCAAGGCATCGAGGTGATGCTGCAATCGGAAAATGGTTTACTGGGTATGG
GCGAGTTCCCAACCGAAGACACCATCGATGCAGACTTAATTAACGCAGGTAAGCAAACCGTTACCGCTGTAGACGGTGCC
TCATTTTTCTCATCCGCGGAAAGCTTTGCCATGATCCGTGGCGGCCATGTGGACTTAACCGTACTTGGCGCATTTGAAGT
CGATGAACAGGGCTCAATCGCCTCGTGGATGATCCCTGGAAAATTAATTAAGGGCATGGGCGGCGCTATGGATTTAGTGG
CAGGCGCAGACAATATTATTGTCACCATGATGCACGCCGACAAGAAAGGCAATTCTAAACTTTTGCCTAAGTGCGAACTG
CCATTAACCGGCTTTGGCTGTATCAAGCGGGTATTAACCGACTTAGCCTTTCTGGAAATTAAAGATGGCGCGTTTCATCT
ATTAGAGCGTGCACCGGGTGTATCTGTTGAAGAAATTGTATCGAAAACAGCTGGCAAGCTAGTAGTGCCTGAACATGTTC
CAGAGATGAGATTTTAA

Upstream 100 bases:

>100_bases
GATCCAAGGTAAGTTCGAGAAACGTATCGAGCAACGTACTGTTAAACCGGTCTCTGATTCAAATACAAACTCAGATGCAA
AAGCATAAGGAGGCAGCACC

Downstream 100 bases:

>100_bases
CGCCCCATGCTCTAACCACCAGCGCTCAAAACACTATGATCTATAAACGCAGCTCAAGAGCTGCGTTTTTTTACTCAGTT
AGCGTTCAGTAATGCCCATC

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MALSREQLAQRVAQELQDGFYVNLGIGIPTLVANYIPQGIEVMLQSENGLLGMGEFPTEDTIDADLINAGKQTVTAVDGA
SFFSSAESFAMIRGGHVDLTVLGAFEVDEQGSIASWMIPGKLIKGMGGAMDLVAGADNIIVTMMHADKKGNSKLLPKCEL
PLTGFGCIKRVLTDLAFLEIKDGAFHLLERAPGVSVEEIVSKTAGKLVVPEHVPEMRF

Sequences:

>Translated_218_residues
MALSREQLAQRVAQELQDGFYVNLGIGIPTLVANYIPQGIEVMLQSENGLLGMGEFPTEDTIDADLINAGKQTVTAVDGA
SFFSSAESFAMIRGGHVDLTVLGAFEVDEQGSIASWMIPGKLIKGMGGAMDLVAGADNIIVTMMHADKKGNSKLLPKCEL
PLTGFGCIKRVLTDLAFLEIKDGAFHLLERAPGVSVEEIVSKTAGKLVVPEHVPEMRF
>Mature_217_residues
ALSREQLAQRVAQELQDGFYVNLGIGIPTLVANYIPQGIEVMLQSENGLLGMGEFPTEDTIDADLINAGKQTVTAVDGAS
FFSSAESFAMIRGGHVDLTVLGAFEVDEQGSIASWMIPGKLIKGMGGAMDLVAGADNIIVTMMHADKKGNSKLLPKCELP
LTGFGCIKRVLTDLAFLEIKDGAFHLLERAPGVSVEEIVSKTAGKLVVPEHVPEMRF

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=213, Percent_Identity=52.5821596244132, Blast_Score=224, Evalue=5e-59,
Organism=Homo sapiens, GI11545841, Length=216, Percent_Identity=48.6111111111111, Blast_Score=216, Evalue=8e-57,
Organism=Escherichia coli, GI1788552, Length=214, Percent_Identity=46.2616822429907, Blast_Score=205, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17531517, Length=216, Percent_Identity=53.7037037037037, Blast_Score=222, Evalue=1e-58,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=55.1401869158878, Blast_Score=245, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=55.1401869158878, Blast_Score=244, Evalue=3e-65,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 23291; Mature: 23160

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSREQLAQRVAQELQDGFYVNLGIGIPTLVANYIPQGIEVMLQSENGLLGMGEFPTED
CCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCC
TIDADLINAGKQTVTAVDGASFFSSAESFAMIRGGHVDLTVLGAFEVDEQGSIASWMIPG
CCCHHHHHCCCCEEEEECCHHHHHHHHHHHEEECCCEEEEEEEEEEECCCCCEEEEECCH
KLIKGMGGAMDLVAGADNIIVTMMHADKKGNSKLLPKCELPLTGFGCIKRVLTDLAFLEI
HHHHCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEE
KDGAFHLLERAPGVSVEEIVSKTAGKLVVPEHVPEMRF
CCCHHHHHHCCCCCCHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure 
ALSREQLAQRVAQELQDGFYVNLGIGIPTLVANYIPQGIEVMLQSENGLLGMGEFPTED
CCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCC
TIDADLINAGKQTVTAVDGASFFSSAESFAMIRGGHVDLTVLGAFEVDEQGSIASWMIPG
CCCHHHHHCCCCEEEEECCHHHHHHHHHHHEEECCCEEEEEEEEEEECCCCCEEEEECCH
KLIKGMGGAMDLVAGADNIIVTMMHADKKGNSKLLPKCELPLTGFGCIKRVLTDLAFLEI
HHHHCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEE
KDGAFHLLERAPGVSVEEIVSKTAGKLVVPEHVPEMRF
CCCHHHHHHCCCCCCHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]