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The map label for this gene is glnS [H]

Identifier: 157962510

GI number: 157962510

Start: 3276320

End: 3277990

Strand: Reverse

Name: glnS [H]

Synonym: Spea_2689

Alternate gene names: 157962510

Gene position: 3277990-3276320 (Counterclockwise)

Preceding gene: 157962511

Following gene: 157962508

Centisome position: 63.35

GC content: 43.15

Gene sequence:

>1671_bases
ATGAGTCATGTGGACACTGAAGTACGTCCTAGTAACTTCATACGTAACATCATAGATGAAGATCTAGCCACGGGTAAGCA
TTCGAGTGTTCAAACTCGTTTTCCGCCAGAACCAAACGGCTTTCTTCATATCGGCCACGCTAAGTCTATCTGCCTGAATT
TTGGCATTGCTCAAGACTATAAAGGTCAATGTAACTTACGTTTTGATGATACCAACCCTGAAAAAGAAGATATCGATTAC
GTTAATTCTATTCAGGCTGATGTGCAGTGGCTCGGTTTTCAGTGGGATGGTGATATTCGTTATTCATCAAACTACTTTGA
TCAACTACATCAGTATGCCGTTGAATTAATCAACAAAGGCCTAGCTTATGTATGTTTCTTAAACGGTGACGAAACTCGTG
AATACCGTGGCACCCTTAAAGAGCCTGGTAAAAACAGCCCTTACCGTGACACAACACCTGAAGAGAACTTAGCGCTATTT
GAAAAAATGCGTTTAGGTGAGTTCAAAGAAGGTGAGTGTTCACTGCGTGCTAAGATTGATATGGCATCACCATTTATGTG
TATGCGTGATCCAATCATCTACCGTATTCGTTTTGCTCATCATCATCAGACTGCCGATAAGTGGTGCATTTACCCAATGT
ACGACTTTACTCACTGTATTTCTGATGCGATTGAAAATATTACTCACTCTCTATGTACGCTTGAATTCCAAGACAACCGT
AGACTGTATGACTGGGTGCTAGACCATTTAGATGATTTCCAAGCGCCTAATAGAACACGTCAGTATGAGTTTTCAAGACT
AAACCTTGAATACACATTGATGTCAAAGCGTAAGCTCAATGACTTAGTGGTTCGCAACTTGGTAACAGGTTGGGATGACG
CGCGTATGCCTACTATCGCAGGCTACCGCCGTCGCGGTTATACAGCAGCTTCTATTCGTGAGTTCTGTAAGCGTATTGGC
GTGACTAAGCAAGATAACATGGTTGAAGTAGCCATGCTTGATGCCTGTATCCGCGAAGAGCTAAATGACTTTGCACCGCG
TGCAATGGCTGTGATTAACCCTGTTAAAGTGGTTATCGAGAACTACCCAGAAGGCCAAGTTGAAATCATCAATGCGGCTA
ATCATCCGAGCAAGGAAGAGATGGGCAAGCGTGAGCTATCTTTTGCTCGTGAACTGTTTATCGATGCCGATGACTTCCGT
GAGGAAGCGAACAAGAAGTACAAACGTTTAGTACAAGGCAAAGAAGTCCGTCTGCGTAATGCTTACGTGATTAAGGCTGA
GCGCTGCGAGAAAGATGCTGAAGGTAACATCACCACTATCTTCTGTACTTATGATGACGAGACTTTGGGTAAAAATCCAA
GCGATGGTCGTAAAGTTAAAGGCGTTATTCATTGGGTCGAAGCGTCAACGGCTAAGCCTGCTGAGCTGCGTATGTACGAA
AGATTGTTCAACGATCCTAACCCTGCTGCAGCAGAATCTGTTGATGAAGTGTTAAACCCACATTCATTGACAATTAAGCA
TGGTCTGGTTGAAGCAAGCTTGGTGAATGCTGAAGCTGAAAAAGCGTATCAGTTTGAGCGTGAAGGTTACTTCTGTGCCG
ATAGCAAGGATTCAAGCAAAGATAATTTAGTGTTTAACCTAACGGTTCCACTAAGAGACACTTTCGAGTAA

Upstream 100 bases:

>100_bases
ACATAAGCGAGTGTTTTTAAGCGGTAGTCGTTGATCCTTTACAAGATCTGTAGGATTATGCGTTTTTAGTCTTTAATCAT
CGTTCGCAAAGAGGAATTCC

Downstream 100 bases:

>100_bases
ATCTTTTTGAGCTTAGCGTTAAGGCTAAAAAAGCCGACATCATGTCGGCTTTTTTGTTGCTTAAACTTCAGTGTTATCTA
TTGATACATAGGTCCGAAAC

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSHVDTEVRPSNFIRNIIDEDLATGKHSSVQTRFPPEPNGFLHIGHAKSICLNFGIAQDYKGQCNLRFDDTNPEKEDIDY
VNSIQADVQWLGFQWDGDIRYSSNYFDQLHQYAVELINKGLAYVCFLNGDETREYRGTLKEPGKNSPYRDTTPEENLALF
EKMRLGEFKEGECSLRAKIDMASPFMCMRDPIIYRIRFAHHHQTADKWCIYPMYDFTHCISDAIENITHSLCTLEFQDNR
RLYDWVLDHLDDFQAPNRTRQYEFSRLNLEYTLMSKRKLNDLVVRNLVTGWDDARMPTIAGYRRRGYTAASIREFCKRIG
VTKQDNMVEVAMLDACIREELNDFAPRAMAVINPVKVVIENYPEGQVEIINAANHPSKEEMGKRELSFARELFIDADDFR
EEANKKYKRLVQGKEVRLRNAYVIKAERCEKDAEGNITTIFCTYDDETLGKNPSDGRKVKGVIHWVEASTAKPAELRMYE
RLFNDPNPAAAESVDEVLNPHSLTIKHGLVEASLVNAEAEKAYQFEREGYFCADSKDSSKDNLVFNLTVPLRDTFE

Sequences:

>Translated_556_residues
MSHVDTEVRPSNFIRNIIDEDLATGKHSSVQTRFPPEPNGFLHIGHAKSICLNFGIAQDYKGQCNLRFDDTNPEKEDIDY
VNSIQADVQWLGFQWDGDIRYSSNYFDQLHQYAVELINKGLAYVCFLNGDETREYRGTLKEPGKNSPYRDTTPEENLALF
EKMRLGEFKEGECSLRAKIDMASPFMCMRDPIIYRIRFAHHHQTADKWCIYPMYDFTHCISDAIENITHSLCTLEFQDNR
RLYDWVLDHLDDFQAPNRTRQYEFSRLNLEYTLMSKRKLNDLVVRNLVTGWDDARMPTIAGYRRRGYTAASIREFCKRIG
VTKQDNMVEVAMLDACIREELNDFAPRAMAVINPVKVVIENYPEGQVEIINAANHPSKEEMGKRELSFARELFIDADDFR
EEANKKYKRLVQGKEVRLRNAYVIKAERCEKDAEGNITTIFCTYDDETLGKNPSDGRKVKGVIHWVEASTAKPAELRMYE
RLFNDPNPAAAESVDEVLNPHSLTIKHGLVEASLVNAEAEKAYQFEREGYFCADSKDSSKDNLVFNLTVPLRDTFE
>Mature_555_residues
SHVDTEVRPSNFIRNIIDEDLATGKHSSVQTRFPPEPNGFLHIGHAKSICLNFGIAQDYKGQCNLRFDDTNPEKEDIDYV
NSIQADVQWLGFQWDGDIRYSSNYFDQLHQYAVELINKGLAYVCFLNGDETREYRGTLKEPGKNSPYRDTTPEENLALFE
KMRLGEFKEGECSLRAKIDMASPFMCMRDPIIYRIRFAHHHQTADKWCIYPMYDFTHCISDAIENITHSLCTLEFQDNRR
LYDWVLDHLDDFQAPNRTRQYEFSRLNLEYTLMSKRKLNDLVVRNLVTGWDDARMPTIAGYRRRGYTAASIREFCKRIGV
TKQDNMVEVAMLDACIREELNDFAPRAMAVINPVKVVIENYPEGQVEIINAANHPSKEEMGKRELSFARELFIDADDFRE
EANKKYKRLVQGKEVRLRNAYVIKAERCEKDAEGNITTIFCTYDDETLGKNPSDGRKVKGVIHWVEASTAKPAELRMYER
LFNDPNPAAAESVDEVLNPHSLTIKHGLVEASLVNAEAEKAYQFEREGYFCADSKDSSKDNLVFNLTVPLRDTFE

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=532, Percent_Identity=45.1127819548872, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI62241042, Length=519, Percent_Identity=33.5260115606936, Blast_Score=266, Evalue=4e-71,
Organism=Escherichia coli, GI1786895, Length=551, Percent_Identity=72.7767695099818, Blast_Score=848, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=119, Percent_Identity=26.890756302521, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=537, Percent_Identity=41.5270018621974, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17511007, Length=533, Percent_Identity=31.5196998123827, Blast_Score=232, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6324742, Length=564, Percent_Identity=38.8297872340425, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321192, Length=539, Percent_Identity=33.9517625231911, Blast_Score=276, Evalue=9e-75,
Organism=Drosophila melanogaster, GI17864488, Length=547, Percent_Identity=40.5850091407678, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24649466, Length=420, Percent_Identity=37.3809523809524, Blast_Score=254, Evalue=9e-68,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 64249; Mature: 64118

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVDTEVRPSNFIRNIIDEDLATGKHSSVQTRFPPEPNGFLHIGHAKSICLNFGIAQDY
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCHHEEHEECCCCCCC
KGQCNLRFDDTNPEKEDIDYVNSIQADVQWLGFQWDGDIRYSSNYFDQLHQYAVELINKG
CCCEEEEECCCCCCHHHHHHHHHHHHCEEEEEEEECCCEEECCHHHHHHHHHHHHHHHCC
LAYVCFLNGDETREYRGTLKEPGKNSPYRDTTPEENLALFEKMRLGEFKEGECSLRAKID
CEEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEC
MASPFMCMRDPIIYRIRFAHHHQTADKWCIYPMYDFTHCISDAIENITHSLCTLEFQDNR
CCCCCHHHCCCEEEEEEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCC
RLYDWVLDHLDDFQAPNRTRQYEFSRLNLEYTLMSKRKLNDLVVRNLVTGWDDARMPTIA
CHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHH
GYRRRGYTAASIREFCKRIGVTKQDNMVEVAMLDACIREELNDFAPRAMAVINPVKVVIE
HHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
NYPEGQVEIINAANHPSKEEMGKRELSFARELFIDADDFREEANKKYKRLVQGKEVRLRN
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEC
AYVIKAERCEKDAEGNITTIFCTYDDETLGKNPSDGRKVKGVIHWVEASTAKPAELRMYE
EEEEEHHHCCCCCCCCEEEEEEEECCHHCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHH
RLFNDPNPAAAESVDEVLNPHSLTIKHGLVEASLVNAEAEKAYQFEREGYFCADSKDSSK
HHCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHCHHHHHHHHHHCCCEEEECCCCCCC
DNLVFNLTVPLRDTFE
CCEEEEEEECCCCCCC
>Mature Secondary Structure 
SHVDTEVRPSNFIRNIIDEDLATGKHSSVQTRFPPEPNGFLHIGHAKSICLNFGIAQDY
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCHHEEHEECCCCCCC
KGQCNLRFDDTNPEKEDIDYVNSIQADVQWLGFQWDGDIRYSSNYFDQLHQYAVELINKG
CCCEEEEECCCCCCHHHHHHHHHHHHCEEEEEEEECCCEEECCHHHHHHHHHHHHHHHCC
LAYVCFLNGDETREYRGTLKEPGKNSPYRDTTPEENLALFEKMRLGEFKEGECSLRAKID
CEEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEC
MASPFMCMRDPIIYRIRFAHHHQTADKWCIYPMYDFTHCISDAIENITHSLCTLEFQDNR
CCCCCHHHCCCEEEEEEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCC
RLYDWVLDHLDDFQAPNRTRQYEFSRLNLEYTLMSKRKLNDLVVRNLVTGWDDARMPTIA
CHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHH
GYRRRGYTAASIREFCKRIGVTKQDNMVEVAMLDACIREELNDFAPRAMAVINPVKVVIE
HHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
NYPEGQVEIINAANHPSKEEMGKRELSFARELFIDADDFREEANKKYKRLVQGKEVRLRN
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEC
AYVIKAERCEKDAEGNITTIFCTYDDETLGKNPSDGRKVKGVIHWVEASTAKPAELRMYE
EEEEEHHHCCCCCCCCEEEEEEEECCHHCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHH
RLFNDPNPAAAESVDEVLNPHSLTIKHGLVEASLVNAEAEKAYQFEREGYFCADSKDSSK
HHCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHCHHHHHHHHHHCCCEEEECCCCCCC
DNLVFNLTVPLRDTFE
CCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]