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The map label for this gene is miaE [H]

Identifier: 157962508

GI number: 157962508

Start: 3274656

End: 3275438

Strand: Reverse

Name: miaE [H]

Synonym: Spea_2687

Alternate gene names: 157962508

Gene position: 3275438-3274656 (Counterclockwise)

Preceding gene: 157962510

Following gene: 157962505

Centisome position: 63.3

GC content: 42.78

Gene sequence:

>783_bases
ATGAATTTAGCTATGCAGCAACTGTTAGCGCCAATTTATACCTTTTTGAAATGCGAAACACCGGATAGCTGGATTGATGA
AGCTAAAAAGCCTGAACGATTAAAAGGACTATTAATTGATCACTGTAACTGTGAATTAAAAGCCGCTCAGACAGCGATGT
TTGTGGTGCGAAAATATGCTGTTGATGAGAAAAGTGGCCAAGTATTGCAAGATTGGGCTAAGCCATATGCTGACTTTGTC
TACAGTCAAGATAGAGATGTAGAGACGTTCTTAGCTAACAACGGTAAAAAGAATGAATTAGCAGCTGAATTAGTTGGGCG
TCGTGACTTTGCCCATAGTGATGACTTGATCGCTAAAATGGTGCGCCTAATTAAAGAGGAGTTTCATCATTTCGAGCAAG
TGCTTGAAATTATGCATAACCGCGGTATTGAGTACCAAAGTGCGCGGGCCGGCCGTTATGCAAAAGGCATGATGAAACAA
GTCCGAACTCATGATCCTGTAACTTTAATCGATAAGCTCATTGTTGGGGCTTTCATTGAGGCTCGATCTTGTGAGCGCTT
TGCCAAACTGGCGCCGCATTTAGATGAGGAGCTGAAGAAGTTTTACATATCACTTTTGCGCTCAGAGGCCCGTCACTACC
AAGACTACTTAACTCTTGCGCAAGCAATATCAGATACTGATATCAGTGACCGCGTCGCACATTTTGCCCAAGTCGAAGCA
GAGTTGATCTCTTCAGATGATGAAGACTTTAAGTTTCATAGTGGCTCACCTGTACTGAGTTAG

Upstream 100 bases:

>100_bases
GAGTCAAGCCTATCGAATTAAGCATAGGTGTTAAGCTAAGACTCTGCTGAAAATAGCTGATAAAATAACGAGCTACTGAC
AGCGCCCTGATGGACCCAAA

Downstream 100 bases:

>100_bases
CAGCTATTTAGGTCTGTTGCTGAACTTGCTAATAATAGGCATGTCGATAAAGAGGCAAGTAGTTAACTACTTGCCGAAAG
GAAGCTCGATTAGCTCGACA

Product: tRNA--hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNLAMQQLLAPIYTFLKCETPDSWIDEAKKPERLKGLLIDHCNCELKAAQTAMFVVRKYAVDEKSGQVLQDWAKPYADFV
YSQDRDVETFLANNGKKNELAAELVGRRDFAHSDDLIAKMVRLIKEEFHHFEQVLEIMHNRGIEYQSARAGRYAKGMMKQ
VRTHDPVTLIDKLIVGAFIEARSCERFAKLAPHLDEELKKFYISLLRSEARHYQDYLTLAQAISDTDISDRVAHFAQVEA
ELISSDDEDFKFHSGSPVLS

Sequences:

>Translated_260_residues
MNLAMQQLLAPIYTFLKCETPDSWIDEAKKPERLKGLLIDHCNCELKAAQTAMFVVRKYAVDEKSGQVLQDWAKPYADFV
YSQDRDVETFLANNGKKNELAAELVGRRDFAHSDDLIAKMVRLIKEEFHHFEQVLEIMHNRGIEYQSARAGRYAKGMMKQ
VRTHDPVTLIDKLIVGAFIEARSCERFAKLAPHLDEELKKFYISLLRSEARHYQDYLTLAQAISDTDISDRVAHFAQVEA
ELISSDDEDFKFHSGSPVLS
>Mature_260_residues
MNLAMQQLLAPIYTFLKCETPDSWIDEAKKPERLKGLLIDHCNCELKAAQTAMFVVRKYAVDEKSGQVLQDWAKPYADFV
YSQDRDVETFLANNGKKNELAAELVGRRDFAHSDDLIAKMVRLIKEEFHHFEQVLEIMHNRGIEYQSARAGRYAKGMMKQ
VRTHDPVTLIDKLIVGAFIEARSCERFAKLAPHLDEELKKFYISLLRSEARHYQDYLTLAQAISDTDISDRVAHFAQVEA
ELISSDDEDFKFHSGSPVLS

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 29844; Mature: 29844

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAMQQLLAPIYTFLKCETPDSWIDEAKKPERLKGLLIDHCNCELKAAQTAMFVVRKYA
CCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VDEKSGQVLQDWAKPYADFVYSQDRDVETFLANNGKKNELAAELVGRRDFAHSDDLIAKM
HCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHH
VRLIKEEFHHFEQVLEIMHNRGIEYQSARAGRYAKGMMKQVRTHDPVTLIDKLIVGAFIE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ARSCERFAKLAPHLDEELKKFYISLLRSEARHYQDYLTLAQAISDTDISDRVAHFAQVEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ELISSDDEDFKFHSGSPVLS
HHHCCCCCCCEECCCCCCCC
>Mature Secondary Structure
MNLAMQQLLAPIYTFLKCETPDSWIDEAKKPERLKGLLIDHCNCELKAAQTAMFVVRKYA
CCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VDEKSGQVLQDWAKPYADFVYSQDRDVETFLANNGKKNELAAELVGRRDFAHSDDLIAKM
HCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHH
VRLIKEEFHHFEQVLEIMHNRGIEYQSARAGRYAKGMMKQVRTHDPVTLIDKLIVGAFIE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ARSCERFAKLAPHLDEELKKFYISLLRSEARHYQDYLTLAQAISDTDISDRVAHFAQVEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ELISSDDEDFKFHSGSPVLS
HHHCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]