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The map label for this gene is hisF [H]

Identifier: 157962283

GI number: 157962283

Start: 3005393

End: 3006166

Strand: Direct

Name: hisF [H]

Synonym: Spea_2462

Alternate gene names: 157962283

Gene position: 3005393-3006166 (Clockwise)

Preceding gene: 157962282

Following gene: 157962284

Centisome position: 58.08

GC content: 47.16

Gene sequence:

>774_bases
ATGTTGGCCAAACGCATAGTTCCCTGCCTCGACGTCAAAGAGGGAAAAGTGGTAAAAGGGGTACAATTTCGAAACCATGA
AATAGTCGGTGATATCGTTCCCCTCGCCCAACGCTACGCAGAAGAAGGCGCCGATGAGCTGGTCTTTTATGATATTACCG
CCAGCGCTCACGATAGAGTGATTGATAAATCTTGGGTGAGCCGGGTCGCTGAACGCATCGATATCCCATTTTGCGTGGCA
GGCGGTATTAGAACCATTGAGCAAGCACGTGAAAAACTCGCCTTTGGTGCCGATAAAATATCTGTCAACTCACCCGCGCT
TACCGATCCGAGTCTAATTGCACGATTACAGGATGAATTTGGCCGTCAGTGTATCGTCATTGGTATCGATTCTTATTATG
ACGCCAGCAGTGACAGCTATATGGTCAAACAGTTTACTGGCGATGAGTCTGCCACTAAAGATACCCAATGGTTTACACAG
AATTGGGTAGAAGAGGTGCAAAAGCAAGGTTGCGGCGAAATAGTACTTAACGTGATGAACCAAGACGGCGTACGCCAAGG
TTACGACATTAAACAACTGTCGATGATCCGTAGCATCTGTGACGTACCATTAATCGCTTCTGGCGGGGCCGGTACCATGG
AGCACTTTGAAGAAGTGTTTAGCCTAGCTAATGTCGATGCAGCACTTGCGGCTAGTGTATTCCACAAAGGGATCATCGAC
ATTCAGGCGCTAAAGCGGTATCTAAAAGATAAAAATATTGCTGTCCGGATCTAA

Upstream 100 bases:

>100_bases
CTTTAGACGACGTTAATGCGGTAAAACAAAGTGGTGCAACAGGCATCATCATAGGAAAAGCCTTATTAATAAATCAGTTT
ACCGTTGAGGAGGCTATTGC

Downstream 100 bases:

>100_bases
TTCAGTGAGACCTTGACCCAAATTAAGGATGTAACATGACAAGTACCAAGCAAAACCAGCAATACGGCGATCTAATCACT
CAATTGGACTGGAAAAAAAC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRIVPCLDVKEGKVVKGVQFRNHEIVGDIVPLAQRYAEEGADELVFYDITASAHDRVIDKSWVSRVAERIDIPFCVA
GGIRTIEQAREKLAFGADKISVNSPALTDPSLIARLQDEFGRQCIVIGIDSYYDASSDSYMVKQFTGDESATKDTQWFTQ
NWVEEVQKQGCGEIVLNVMNQDGVRQGYDIKQLSMIRSICDVPLIASGGAGTMEHFEEVFSLANVDAALAASVFHKGIID
IQALKRYLKDKNIAVRI

Sequences:

>Translated_257_residues
MLAKRIVPCLDVKEGKVVKGVQFRNHEIVGDIVPLAQRYAEEGADELVFYDITASAHDRVIDKSWVSRVAERIDIPFCVA
GGIRTIEQAREKLAFGADKISVNSPALTDPSLIARLQDEFGRQCIVIGIDSYYDASSDSYMVKQFTGDESATKDTQWFTQ
NWVEEVQKQGCGEIVLNVMNQDGVRQGYDIKQLSMIRSICDVPLIASGGAGTMEHFEEVFSLANVDAALAASVFHKGIID
IQALKRYLKDKNIAVRI
>Mature_257_residues
MLAKRIVPCLDVKEGKVVKGVQFRNHEIVGDIVPLAQRYAEEGADELVFYDITASAHDRVIDKSWVSRVAERIDIPFCVA
GGIRTIEQAREKLAFGADKISVNSPALTDPSLIARLQDEFGRQCIVIGIDSYYDASSDSYMVKQFTGDESATKDTQWFTQ
NWVEEVQKQGCGEIVLNVMNQDGVRQGYDIKQLSMIRSICDVPLIASGGAGTMEHFEEVFSLANVDAALAASVFHKGIID
IQALKRYLKDKNIAVRI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=71.5953307392996, Blast_Score=390, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28505; Mature: 28505

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIVPCLDVKEGKVVKGVQFRNHEIVGDIVPLAQRYAEEGADELVFYDITASAHDRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
IDKSWVSRVAERIDIPFCVAGGIRTIEQAREKLAFGADKISVNSPALTDPSLIARLQDEF
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHH
GRQCIVIGIDSYYDASSDSYMVKQFTGDESATKDTQWFTQNWVEEVQKQGCGEIVLNVMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHC
QDGVRQGYDIKQLSMIRSICDVPLIASGGAGTMEHFEEVFSLANVDAALAASVFHKGIID
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHH
IQALKRYLKDKNIAVRI
HHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MLAKRIVPCLDVKEGKVVKGVQFRNHEIVGDIVPLAQRYAEEGADELVFYDITASAHDRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
IDKSWVSRVAERIDIPFCVAGGIRTIEQAREKLAFGADKISVNSPALTDPSLIARLQDEF
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHH
GRQCIVIGIDSYYDASSDSYMVKQFTGDESATKDTQWFTQNWVEEVQKQGCGEIVLNVMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHC
QDGVRQGYDIKQLSMIRSICDVPLIASGGAGTMEHFEEVFSLANVDAALAASVFHKGIID
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHH
IQALKRYLKDKNIAVRI
HHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA