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The map label for this gene is hisH [H]

Identifier: 157962281

GI number: 157962281

Start: 3003934

End: 3004677

Strand: Direct

Name: hisH [H]

Synonym: Spea_2460

Alternate gene names: 157962281

Gene position: 3003934-3004677 (Clockwise)

Preceding gene: 157962280

Following gene: 157962282

Centisome position: 58.05

GC content: 48.79

Gene sequence:

>744_bases
ATGAGCCAACAAGATAAAGCAGGCTTGAGCGATGATCGTTCATTGAACAATAACAAAGCCATAGTCGGCAACACCGTTAT
TGTCGACACAGGTTGCGCCAATTTAAGCTCTGTGCGTTACGCCTTCGAACGTCTAATGACGGATATTGAGCGCGAGCGCC
TATTTGTCACCGATGATATCGAGATGATAAGAGCTGCCGAGCGGGTGATTTTGCCTGGTGTCGGCACTGCTGCGGCTGCC
ATGAGCGCTTTACAAAGCAAAGGCTTAGTACAACTTATCCCTGAGTTGACCCAACCGGTACTCGGTGTGTGTTTAGGCAT
GCAGATGCTGACAAGAGTATCTAAAGAACGTGGCAGCCAAAGTGAAGACTGTCCGTGTCTCAATATCATACCGACTGATA
TCAATTTACTCGACACCAAAGGACTGCCACTGCCGCATATGGGCTGGAATCAGCTAGTGCCAGGCTCACATCCCATCTTT
GACGGGATCACAGCGGGCAGTTATGTGTATTTTGTCCACAGCTACCGAGCACCAATCAGTGATTACACTATCGCTACGGC
GCAGCACGGAGAAACATTTAGCGCGGCAATTGCCAAAGATAATTTTATCGGTCTGCAGTTCCACCCTGAAAAAAGCGCGG
AAGTCGGTGCAAGAATGCTTAAAAACTTCCTGACTATGGATCAAGCGACGTTCAGTCCAACAAATAACCAACTGGCGTAT
GGCCAGCAAGAGGCGCAGTTATGA

Upstream 100 bases:

>100_bases
ATCACCACAAGGTTGAGAGCTTATTTAAAGTCCTTGGTCGTACTCTTCGACAAGCAGTAGCGATAGAAGGTGATGCGCTG
CCATCAAGTAAAGGTGTGTT

Downstream 100 bases:

>100_bases
TTATTCCAGCAATCGATCTTATCGATGGTCAAGTTGTTCGCCTCTATCAAGGGGATTATGCCCAGCAGACCACCTTTAAT
TTAAGCCCATTAGATCAGCT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSQQDKAGLSDDRSLNNNKAIVGNTVIVDTGCANLSSVRYAFERLMTDIERERLFVTDDIEMIRAAERVILPGVGTAAAA
MSALQSKGLVQLIPELTQPVLGVCLGMQMLTRVSKERGSQSEDCPCLNIIPTDINLLDTKGLPLPHMGWNQLVPGSHPIF
DGITAGSYVYFVHSYRAPISDYTIATAQHGETFSAAIAKDNFIGLQFHPEKSAEVGARMLKNFLTMDQATFSPTNNQLAY
GQQEAQL

Sequences:

>Translated_247_residues
MSQQDKAGLSDDRSLNNNKAIVGNTVIVDTGCANLSSVRYAFERLMTDIERERLFVTDDIEMIRAAERVILPGVGTAAAA
MSALQSKGLVQLIPELTQPVLGVCLGMQMLTRVSKERGSQSEDCPCLNIIPTDINLLDTKGLPLPHMGWNQLVPGSHPIF
DGITAGSYVYFVHSYRAPISDYTIATAQHGETFSAAIAKDNFIGLQFHPEKSAEVGARMLKNFLTMDQATFSPTNNQLAY
GQQEAQL
>Mature_246_residues
SQQDKAGLSDDRSLNNNKAIVGNTVIVDTGCANLSSVRYAFERLMTDIERERLFVTDDIEMIRAAERVILPGVGTAAAAM
SALQSKGLVQLIPELTQPVLGVCLGMQMLTRVSKERGSQSEDCPCLNIIPTDINLLDTKGLPLPHMGWNQLVPGSHPIFD
GITAGSYVYFVHSYRAPISDYTIATAQHGETFSAAIAKDNFIGLQFHPEKSAEVGARMLKNFLTMDQATFSPTNNQLAYG
QQEAQL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=204, Percent_Identity=49.0196078431373, Blast_Score=196, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=32.5471698113208, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 26921; Mature: 26790

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQDKAGLSDDRSLNNNKAIVGNTVIVDTGCANLSSVRYAFERLMTDIERERLFVTDDI
CCCCCCCCCCCCCCCCCCCEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCEEEEECCH
EMIRAAERVILPGVGTAAAAMSALQSKGLVQLIPELTQPVLGVCLGMQMLTRVSKERGSQ
HHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SEDCPCLNIIPTDINLLDTKGLPLPHMGWNQLVPGSHPIFDGITAGSYVYFVHSYRAPIS
CCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
DYTIATAQHGETFSAAIAKDNFIGLQFHPEKSAEVGARMLKNFLTMDQATFSPTNNQLAY
CCEEEECCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
GQQEAQL
CHHHCCC
>Mature Secondary Structure 
SQQDKAGLSDDRSLNNNKAIVGNTVIVDTGCANLSSVRYAFERLMTDIERERLFVTDDI
CCCCCCCCCCCCCCCCCCEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCEEEEECCH
EMIRAAERVILPGVGTAAAAMSALQSKGLVQLIPELTQPVLGVCLGMQMLTRVSKERGSQ
HHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SEDCPCLNIIPTDINLLDTKGLPLPHMGWNQLVPGSHPIFDGITAGSYVYFVHSYRAPIS
CCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
DYTIATAQHGETFSAAIAKDNFIGLQFHPEKSAEVGARMLKNFLTMDQATFSPTNNQLAY
CCEEEECCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
GQQEAQL
CHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA