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The map label for this gene is hisB [H]

Identifier: 157962280

GI number: 157962280

Start: 3002843

End: 3003937

Strand: Direct

Name: hisB [H]

Synonym: Spea_2459

Alternate gene names: 157962280

Gene position: 3002843-3003937 (Clockwise)

Preceding gene: 157962279

Following gene: 157962281

Centisome position: 58.03

GC content: 46.76

Gene sequence:

>1095_bases
ATGAAGCAGAAAATTTTATTTATCGACCGTGATGGCACCCTAATCGAAGAGCCAGTTACAGATAAACAAGTCGATAGTCT
AGCTAAACTCGTGTTTGAGCCAAATGTGATCCCTGCGCTATTAAAGCTGCAGAATGCAGGTTACAGACTGGTCATGATCA
GTAATCAGGATGGTCTTGGTACGCCTTCTTTCCCAAAAGATGATTTTGACGCGCCGCAAGATATGATGATGCAGATCTTA
AATAGCCAAGGTGTGTTATTTGATGACGTGCTTATCTGCCCACACTTTGATGATGAAAACTGTAGCTGCCGTAAACCTAA
GCTTGGCCTAGTTAAAGGTTACCTGACTTCTGGCAAGATAGACTTTACCCAGTCAGCCGTGATTGGCGATCGTGAAACTG
ACCTTGGCCTTGCAGATTCAATGGGCATATTAGGGCTGCAATACCACCCTGAGACGCTTAATTGGAACGCTATTGCCGCT
AAGTTGCTGAACAAGAACCGCTGCGCAACTGTCACTCGCACCACTAAAGAAACCGATATCCGCGTCACTGTTGATTTAGA
CGCCGCAGATAAAGGTGCGATCAACACAGGCATAGGTTTCTTTGACCATATGCTGGAACAGATCTCGACACACGGTAATT
TCAAGCTAGATGTTCAGGTCGATGGCGACTTAGAGATTGACGATCACCACAGTGTTGAAGATACGGCGCTCGCTATCGGT
GATGCATTGCGACAAGCCCTTGGTGATAAACGCGGCATTGCACGCTTTGGTAACTCACTACCTAATGATGTATTCCAGCA
GGCAATTCCTATGGATGAAGCCAGTGCTAGCTGTCTATTAGACTTATCTGGCCGTCCTTTTATCAAGTTTGACGGCGAGT
TTGACCGTGAGCTTGTGGGGCAGCTGTCTACTGAAATGGTGCCGCACTTCTTCCGCTCATTCGCCGATGGCCTACGCTGC
ACCCTACACATCAGCACTCAAGGTGATAATGATCACCACAAGGTTGAGAGCTTATTTAAAGTCCTTGGTCGTACTCTTCG
ACAAGCAGTAGCGATAGAAGGTGATGCGCTGCCATCAAGTAAAGGTGTGTTATGA

Upstream 100 bases:

>100_bases
TCAACAAAAATAAAATAGCTAGAAAGCTAATAATTAAAAAATAACGATAGCTTGACTGGTATTGATTTAGTTAAGCGCAA
ATTTGAAAGGTATACCCGCT

Downstream 100 bases:

>100_bases
GCCAACAAGATAAAGCAGGCTTGAGCGATGATCGTTCATTGAACAATAACAAAGCCATAGTCGGCAACACCGTTATTGTC
GACACAGGTTGCGCCAATTT

Product: imidazole glycerol-phosphate dehydratase/histidinol phosphatase

Products: NA

Alternate protein names: Histidinol-phosphatase; Imidazoleglycerol-phosphate dehydratase; IGPD [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKQKILFIDRDGTLIEEPVTDKQVDSLAKLVFEPNVIPALLKLQNAGYRLVMISNQDGLGTPSFPKDDFDAPQDMMMQIL
NSQGVLFDDVLICPHFDDENCSCRKPKLGLVKGYLTSGKIDFTQSAVIGDRETDLGLADSMGILGLQYHPETLNWNAIAA
KLLNKNRCATVTRTTKETDIRVTVDLDAADKGAINTGIGFFDHMLEQISTHGNFKLDVQVDGDLEIDDHHSVEDTALAIG
DALRQALGDKRGIARFGNSLPNDVFQQAIPMDEASASCLLDLSGRPFIKFDGEFDRELVGQLSTEMVPHFFRSFADGLRC
TLHISTQGDNDHHKVESLFKVLGRTLRQAVAIEGDALPSSKGVL

Sequences:

>Translated_364_residues
MKQKILFIDRDGTLIEEPVTDKQVDSLAKLVFEPNVIPALLKLQNAGYRLVMISNQDGLGTPSFPKDDFDAPQDMMMQIL
NSQGVLFDDVLICPHFDDENCSCRKPKLGLVKGYLTSGKIDFTQSAVIGDRETDLGLADSMGILGLQYHPETLNWNAIAA
KLLNKNRCATVTRTTKETDIRVTVDLDAADKGAINTGIGFFDHMLEQISTHGNFKLDVQVDGDLEIDDHHSVEDTALAIG
DALRQALGDKRGIARFGNSLPNDVFQQAIPMDEASASCLLDLSGRPFIKFDGEFDRELVGQLSTEMVPHFFRSFADGLRC
TLHISTQGDNDHHKVESLFKVLGRTLRQAVAIEGDALPSSKGVL
>Mature_364_residues
MKQKILFIDRDGTLIEEPVTDKQVDSLAKLVFEPNVIPALLKLQNAGYRLVMISNQDGLGTPSFPKDDFDAPQDMMMQIL
NSQGVLFDDVLICPHFDDENCSCRKPKLGLVKGYLTSGKIDFTQSAVIGDRETDLGLADSMGILGLQYHPETLNWNAIAA
KLLNKNRCATVTRTTKETDIRVTVDLDAADKGAINTGIGFFDHMLEQISTHGNFKLDVQVDGDLEIDDHHSVEDTALAIG
DALRQALGDKRGIARFGNSLPNDVFQQAIPMDEASASCLLDLSGRPFIKFDGEFDRELVGQLSTEMVPHFFRSFADGLRC
TLHISTQGDNDHHKVESLFKVLGRTLRQAVAIEGDALPSSKGVL

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI87082027, Length=364, Percent_Identity=60.989010989011, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1786399, Length=140, Percent_Identity=29.2857142857143, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324776, Length=222, Percent_Identity=39.6396396396396, Blast_Score=158, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006549
- InterPro:   IPR020566
- InterPro:   IPR005954
- InterPro:   IPR006543
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR013954
- InterPro:   IPR020568 [H]

Pfam domain/function: PF00475 IGPD; PF08645 PNK3P [H]

EC number: =3.1.3.15; =4.2.1.19 [H]

Molecular weight: Translated: 40032; Mature: 40032

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00954 IGP_DEHYDRATASE_1 ; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKILFIDRDGTLIEEPVTDKQVDSLAKLVFEPNVIPALLKLQNAGYRLVMISNQDGLG
CCCEEEEEECCCCEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEECCCCCC
TPSFPKDDFDAPQDMMMQILNSQGVLFDDVLICPHFDDENCSCRKPKLGLVKGYLTSGKI
CCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCE
DFTQSAVIGDRETDLGLADSMGILGLQYHPETLNWNAIAAKLLNKNRCATVTRTTKETDI
ECCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCEE
RVTVDLDAADKGAINTGIGFFDHMLEQISTHGNFKLDVQVDGDLEIDDHHSVEDTALAIG
EEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCCHHHHHHHH
DALRQALGDKRGIARFGNSLPNDVFQQAIPMDEASASCLLDLSGRPFIKFDGEFDRELVG
HHHHHHHCCCHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCCCHHHHH
QLSTEMVPHFFRSFADGLRCTLHISTQGDNDHHKVESLFKVLGRTLRQAVAIEGDALPSS
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KGVL
CCCC
>Mature Secondary Structure
MKQKILFIDRDGTLIEEPVTDKQVDSLAKLVFEPNVIPALLKLQNAGYRLVMISNQDGLG
CCCEEEEEECCCCEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEECCCCCC
TPSFPKDDFDAPQDMMMQILNSQGVLFDDVLICPHFDDENCSCRKPKLGLVKGYLTSGKI
CCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCE
DFTQSAVIGDRETDLGLADSMGILGLQYHPETLNWNAIAAKLLNKNRCATVTRTTKETDI
ECCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCEE
RVTVDLDAADKGAINTGIGFFDHMLEQISTHGNFKLDVQVDGDLEIDDHHSVEDTALAIG
EEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCCHHHHHHHH
DALRQALGDKRGIARFGNSLPNDVFQQAIPMDEASASCLLDLSGRPFIKFDGEFDRELVG
HHHHHHHCCCHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCCCHHHHH
QLSTEMVPHFFRSFADGLRCTLHISTQGDNDHHKVESLFKVLGRTLRQAVAIEGDALPSS
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KGVL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]