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The map label for this gene is ycdT [H]

Identifier: 157962018

GI number: 157962018

Start: 2666018

End: 2667334

Strand: Direct

Name: ycdT [H]

Synonym: Spea_2197

Alternate gene names: 157962018

Gene position: 2666018-2667334 (Clockwise)

Preceding gene: 157962015

Following gene: 157962019

Centisome position: 51.52

GC content: 33.11

Gene sequence:

>1317_bases
ATGTTAAATGATATGACAAGAAGATGTGCGCTCAGCATTTGTTTATCTTTTATTGTTTCTATATGTATAGTTAATTTATT
CTTGACTGTTAATAAAGGTAAGTTGAATAGTAAGTTTTTAACTAACATCCTTTTTATTAGTGATGCAATATACCGTTTTA
ATGTAAACAGCCTTAACAGTAAGTACATTTCTCTACCATCAAATGATTTAAAACAGGGGGTCATAGTAAAAAATTCTGAA
GCTAAAAAAATTCGAGACCTATCTGAACATATAGATAATCTATTTGCTGTATTGAAAATCAATCCAATCATATCTAGCAA
CTTATGGACGGTCGCGAATGTATACCCAGAGTATGTCATTCCAAGGCCGTTTAGAGAGGAGTACATAAACCTTTTTGATA
AGGATTTTGCTGAGGAAAACTACCTAGAACGTATTTTAAAATTAGAAAATATTGAAGAAACACTAGAAGGAAGTGGTTTA
ACTGTATTTGAAACAGTGAGAGTTTTCGGTCCTTATATAGAAGAGGGAACCAATGAAGAGTTATTTTCAATTTATTACCC
ACTATATTTAGAAGGCGTTGTTTTTTCTGTTTTAGTTATTGATGTAAAAAGTAATTTTTTAAATGATTTCATAGAAAATT
TTAATGATGAAGAATTTACTTGGTTTGAAATGTCAGATAAAGACCGTCAAAACATCAGTACGATTATATACGAATCCTTA
GCACTTAAGACCGAAGATAGAAAAGTGGGCCTAACAATAAAAACACCTACACTGTACAACTCTATTGCCATTTTAATATG
TACGCTAATCATACACGGCTGTTTTTTAATTGCTGCATACATTAGAAAGTTTTATGTCGAAAGCAGCATAGATAGGTTAT
CAGGCTTCTATCGAAAAGATGGTTTTATGAGTAAAAAAATGTCTGTTAACTCTATGTGCCTAATTGATATCGATCACTTT
AAGAAAATCAATGATACATATGGACACGTTGTTGGTGACATTGTGATTGCAGAAGTGGCTAATCGAATAAAAAACTCCAT
GCGCTCAACTGATCTCGCTATCCGCTGGGGTGGCGAAGAGTTTGTGGTTATTTTTAATGGTGAACTGTCTTTCGAGCAAT
TCAATAGCAAAGCCAATGGTTTATTGAGCACGATAAATAGTGAAACCATCAATGATATAGCCGTCACTATATCTATAGGT
GCTATTTACTCCCAGTCTGAAATGAAATTGCAGGAAGCATTTCATCTTTCTGACGAAGCTCTTTATGAGTCGAAGAACTC
TGGACGAAATAAAGTCACTATTCATCGTCATAGCTGA

Upstream 100 bases:

>100_bases
TATCGCTTCAAATTTATGTAAGATTATTCTTAGTGAATAATGAGAAGTATTTGTTGATCAATGAGTTGTGGTTGTGACTC
TAGTCTATTTCTATTGCTGG

Downstream 100 bases:

>100_bases
GTGATAGTGCATCTAATTATCACGTAGCATTCAACAATGCTAAGCTTAGCGAGCAATCCATTGCAATGTTTCAAGATAAG
CAATTCAAACTAATCAAATT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MLNDMTRRCALSICLSFIVSICIVNLFLTVNKGKLNSKFLTNILFISDAIYRFNVNSLNSKYISLPSNDLKQGVIVKNSE
AKKIRDLSEHIDNLFAVLKINPIISSNLWTVANVYPEYVIPRPFREEYINLFDKDFAEENYLERILKLENIEETLEGSGL
TVFETVRVFGPYIEEGTNEELFSIYYPLYLEGVVFSVLVIDVKSNFLNDFIENFNDEEFTWFEMSDKDRQNISTIIYESL
ALKTEDRKVGLTIKTPTLYNSIAILICTLIIHGCFLIAAYIRKFYVESSIDRLSGFYRKDGFMSKKMSVNSMCLIDIDHF
KKINDTYGHVVGDIVIAEVANRIKNSMRSTDLAIRWGGEEFVVIFNGELSFEQFNSKANGLLSTINSETINDIAVTISIG
AIYSQSEMKLQEAFHLSDEALYESKNSGRNKVTIHRHS

Sequences:

>Translated_438_residues
MLNDMTRRCALSICLSFIVSICIVNLFLTVNKGKLNSKFLTNILFISDAIYRFNVNSLNSKYISLPSNDLKQGVIVKNSE
AKKIRDLSEHIDNLFAVLKINPIISSNLWTVANVYPEYVIPRPFREEYINLFDKDFAEENYLERILKLENIEETLEGSGL
TVFETVRVFGPYIEEGTNEELFSIYYPLYLEGVVFSVLVIDVKSNFLNDFIENFNDEEFTWFEMSDKDRQNISTIIYESL
ALKTEDRKVGLTIKTPTLYNSIAILICTLIIHGCFLIAAYIRKFYVESSIDRLSGFYRKDGFMSKKMSVNSMCLIDIDHF
KKINDTYGHVVGDIVIAEVANRIKNSMRSTDLAIRWGGEEFVVIFNGELSFEQFNSKANGLLSTINSETINDIAVTISIG
AIYSQSEMKLQEAFHLSDEALYESKNSGRNKVTIHRHS
>Mature_438_residues
MLNDMTRRCALSICLSFIVSICIVNLFLTVNKGKLNSKFLTNILFISDAIYRFNVNSLNSKYISLPSNDLKQGVIVKNSE
AKKIRDLSEHIDNLFAVLKINPIISSNLWTVANVYPEYVIPRPFREEYINLFDKDFAEENYLERILKLENIEETLEGSGL
TVFETVRVFGPYIEEGTNEELFSIYYPLYLEGVVFSVLVIDVKSNFLNDFIENFNDEEFTWFEMSDKDRQNISTIIYESL
ALKTEDRKVGLTIKTPTLYNSIAILICTLIIHGCFLIAAYIRKFYVESSIDRLSGFYRKDGFMSKKMSVNSMCLIDIDHF
KKINDTYGHVVGDIVIAEVANRIKNSMRSTDLAIRWGGEEFVVIFNGELSFEQFNSKANGLLSTINSETINDIAVTISIG
AIYSQSEMKLQEAFHLSDEALYESKNSGRNKVTIHRHS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=129, Percent_Identity=44.1860465116279, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081881, Length=123, Percent_Identity=38.2113821138211, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=28.6549707602339, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI145693134, Length=148, Percent_Identity=31.7567567567568, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=192, Percent_Identity=29.1666666666667, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787056, Length=169, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787816, Length=124, Percent_Identity=31.4516129032258, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787802, Length=217, Percent_Identity=28.110599078341, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788085, Length=130, Percent_Identity=32.3076923076923, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788381, Length=132, Percent_Identity=28.7878787878788, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081974, Length=313, Percent_Identity=26.1980830670927, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50140; Mature: 50140

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNDMTRRCALSICLSFIVSICIVNLFLTVNKGKLNSKFLTNILFISDAIYRFNVNSLNS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHEEEECCCCCC
KYISLPSNDLKQGVIVKNSEAKKIRDLSEHIDNLFAVLKINPIISSNLWTVANVYPEYVI
CEEECCCCHHCCCEEEECCCHHHHHHHHHHHHHHEEHEEECCHHCCCCEEHHHCCCCCCC
PRPFREEYINLFDKDFAEENYLERILKLENIEETLEGSGLTVFETVRVFGPYIEEGTNEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHCCCCCC
LFSIYYPLYLEGVVFSVLVIDVKSNFLNDFIENFNDEEFTWFEMSDKDRQNISTIIYESL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
ALKTEDRKVGLTIKTPTLYNSIAILICTLIIHGCFLIAAYIRKFYVESSIDRLSGFYRKD
HEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFMSKKMSVNSMCLIDIDHFKKINDTYGHVVGDIVIAEVANRIKNSMRSTDLAIRWGGEE
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCE
FVVIFNGELSFEQFNSKANGLLSTINSETINDIAVTISIGAIYSQSEMKLQEAFHLSDEA
EEEEECCCCCHHHHCCHHHHHHHHHCCCCCCEEEEEEEECCEECHHHHHHHHHHCCCHHH
LYESKNSGRNKVTIHRHS
HHHCCCCCCCEEEEEECC
>Mature Secondary Structure
MLNDMTRRCALSICLSFIVSICIVNLFLTVNKGKLNSKFLTNILFISDAIYRFNVNSLNS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHEEEECCCCCC
KYISLPSNDLKQGVIVKNSEAKKIRDLSEHIDNLFAVLKINPIISSNLWTVANVYPEYVI
CEEECCCCHHCCCEEEECCCHHHHHHHHHHHHHHEEHEEECCHHCCCCEEHHHCCCCCCC
PRPFREEYINLFDKDFAEENYLERILKLENIEETLEGSGLTVFETVRVFGPYIEEGTNEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHCCCCCC
LFSIYYPLYLEGVVFSVLVIDVKSNFLNDFIENFNDEEFTWFEMSDKDRQNISTIIYESL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
ALKTEDRKVGLTIKTPTLYNSIAILICTLIIHGCFLIAAYIRKFYVESSIDRLSGFYRKD
HEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFMSKKMSVNSMCLIDIDHFKKINDTYGHVVGDIVIAEVANRIKNSMRSTDLAIRWGGEE
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCE
FVVIFNGELSFEQFNSKANGLLSTINSETINDIAVTISIGAIYSQSEMKLQEAFHLSDEA
EEEEECCCCCHHHHCCHHHHHHHHHCCCCCCEEEEEEEECCEECHHHHHHHHHHCCCHHH
LYESKNSGRNKVTIHRHS
HHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]