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The map label for this gene is oprM [H]

Identifier: 157962015

GI number: 157962015

Start: 2662368

End: 2663924

Strand: Direct

Name: oprM [H]

Synonym: Spea_2194

Alternate gene names: 157962015

Gene position: 2662368-2663924 (Clockwise)

Preceding gene: 157962014

Following gene: 157962018

Centisome position: 51.45

GC content: 45.54

Gene sequence:

>1557_bases
ATGCCTAATCAATTCAATATCAGTCAATTCAATATCACTCAAGCCTATATCAAGCATAAGATGCCAATCGGTGGTGTTCG
AGCATTTCGAGTGAACACCTCAGTATTGAGTGTCGCCTGTGGGATTTTGCTACTTTCTGGTTGCGCCATGGGGCCTGATT
ACCAGAGACCCGATCTTGAAATACCGGAGCAATTTCACCACGAGTTGCAGCAAACCGATGAGCAGAATGTTGGGCTGAAG
CATTGGCGTGATTTTTATCTCGATCCTGATCTACAGGTATTGATAGAACATGCTCTCGATCAAAACCTAGATTTAGAGGG
AGTGCGCTCAAGGTTGTTAGCCGCAAGAGCTGGGGTTACGGTTACTGACGCAGCACTATTTCCAGTTGTAGGACTTGATG
CAAATGGTGAGCGAGCATTAGATAGCGCGTTGACCAGTACTGATCCCGTTAAAGAAGATACTTTTTATGTTGCCGGTACG
GTTTCGTGGGAATTGGATCTTTGGGGAGCGAATCGTCGAAGAAGTGAAGCTGAATTTGCCAACTTCATCTCTTCACAAGA
AGCACTTAATTTAGCCACCATCAGTCTAATCAGTGATGTTGCCAGCCGTTATTATGAATGGCTCGATGTTGAGCAACGTT
ATCAAATATCGCTTAATACTGTCGAGCTACGTAAAAAAGAGCGAGATCTTGCTAGGCTACGTAAGCAAAATGGTGTGATA
TCGGGCCTGCAAGTTAGGCAGGCGGAGGTGGAGTATCAAAGTGCCAGAGTCACACTACCGGATCTTGATTATGAGCGAAG
TGAAAAGGCGAATCAGCTGCGAATATTACTGGGCGAATTTACGTATCCCCTTATTCCTAAAACTGAAGCGCAGTTAAAAG
CTGAAGGAGCATTGTTTCCAGACTCATTTGCAGTGGGTATTCCATCACAGATGCTATCACAACGCCCTGATGTAAAGTCC
GCTGAGCAAGCTCTTGTTGCAGCCAATGCCAATGTCGGTGTCGCTAAAACAGCATTCTTTCCTAAATTTACCATTAGCGG
CAGCTATGGTTCTGAGTCTGAAAGCCTCAGTGACATATTTGATAGCCAGGGGCTGACTTGGTCGCTTTTAGGCGGGGTAA
CCGCTCCGATTTTCAATGCAGGTAAGATCTCAGCTCAATATGATATTGCTCAGGAAAATGCCAAACAGGCTATGCTCAGT
TATAAAAGTACGGTGCTTGGGGCATACTTTGAAGTAAATGATGCGATGAACAGTTTCAGACGCTCTGAGCTCGCGATTGA
CGCTCAGCAGGAATTAGTCGTGGCATCAACTGAATATACCCGCTTAGCAACGTTAAGATACCGAAATGGTGTGTCGAGTT
CGTTGGATCTGATGGATGCACAGCGAAGTCTGTTTAGCGCCGAACTGACTCTAAGTCAGGTGAAACGTGATCGATTATTG
TCGATGATTACGCTTTATCGTGCGCTGGGTGGGGGAGTACTGACCCGTGATGAGATGAAGGCTGAACTTGAAAAACCGAC
TGGCAAGCAACAAGTTGAAGAAGTCACTAATCAATAA

Upstream 100 bases:

>100_bases
ACCATTGGCATCATTATGGTACCGCTGTTTTTTGTGACTACGGCAGGCTGGGTTCGCTCTAAAATCACCGGCAAAGCCGA
TACAGTGGAGGTGTCGACTC

Downstream 100 bases:

>100_bases
ATAATAAAAATTTTTGAGCGATTAGTCCTCCAATTTCGGGAGGCTAGTCGCAGAGCTCTGCTAGGATACACTGCTCCTAC
AACTAAGCTCCTAACTCAGC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MPNQFNISQFNITQAYIKHKMPIGGVRAFRVNTSVLSVACGILLLSGCAMGPDYQRPDLEIPEQFHHELQQTDEQNVGLK
HWRDFYLDPDLQVLIEHALDQNLDLEGVRSRLLAARAGVTVTDAALFPVVGLDANGERALDSALTSTDPVKEDTFYVAGT
VSWELDLWGANRRRSEAEFANFISSQEALNLATISLISDVASRYYEWLDVEQRYQISLNTVELRKKERDLARLRKQNGVI
SGLQVRQAEVEYQSARVTLPDLDYERSEKANQLRILLGEFTYPLIPKTEAQLKAEGALFPDSFAVGIPSQMLSQRPDVKS
AEQALVAANANVGVAKTAFFPKFTISGSYGSESESLSDIFDSQGLTWSLLGGVTAPIFNAGKISAQYDIAQENAKQAMLS
YKSTVLGAYFEVNDAMNSFRRSELAIDAQQELVVASTEYTRLATLRYRNGVSSSLDLMDAQRSLFSAELTLSQVKRDRLL
SMITLYRALGGGVLTRDEMKAELEKPTGKQQVEEVTNQ

Sequences:

>Translated_518_residues
MPNQFNISQFNITQAYIKHKMPIGGVRAFRVNTSVLSVACGILLLSGCAMGPDYQRPDLEIPEQFHHELQQTDEQNVGLK
HWRDFYLDPDLQVLIEHALDQNLDLEGVRSRLLAARAGVTVTDAALFPVVGLDANGERALDSALTSTDPVKEDTFYVAGT
VSWELDLWGANRRRSEAEFANFISSQEALNLATISLISDVASRYYEWLDVEQRYQISLNTVELRKKERDLARLRKQNGVI
SGLQVRQAEVEYQSARVTLPDLDYERSEKANQLRILLGEFTYPLIPKTEAQLKAEGALFPDSFAVGIPSQMLSQRPDVKS
AEQALVAANANVGVAKTAFFPKFTISGSYGSESESLSDIFDSQGLTWSLLGGVTAPIFNAGKISAQYDIAQENAKQAMLS
YKSTVLGAYFEVNDAMNSFRRSELAIDAQQELVVASTEYTRLATLRYRNGVSSSLDLMDAQRSLFSAELTLSQVKRDRLL
SMITLYRALGGGVLTRDEMKAELEKPTGKQQVEEVTNQ
>Mature_517_residues
PNQFNISQFNITQAYIKHKMPIGGVRAFRVNTSVLSVACGILLLSGCAMGPDYQRPDLEIPEQFHHELQQTDEQNVGLKH
WRDFYLDPDLQVLIEHALDQNLDLEGVRSRLLAARAGVTVTDAALFPVVGLDANGERALDSALTSTDPVKEDTFYVAGTV
SWELDLWGANRRRSEAEFANFISSQEALNLATISLISDVASRYYEWLDVEQRYQISLNTVELRKKERDLARLRKQNGVIS
GLQVRQAEVEYQSARVTLPDLDYERSEKANQLRILLGEFTYPLIPKTEAQLKAEGALFPDSFAVGIPSQMLSQRPDVKSA
EQALVAANANVGVAKTAFFPKFTISGSYGSESESLSDIFDSQGLTWSLLGGVTAPIFNAGKISAQYDIAQENAKQAMLSY
KSTVLGAYFEVNDAMNSFRRSELAIDAQQELVVASTEYTRLATLRYRNGVSSSLDLMDAQRSLFSAELTLSQVKRDRLLS
MITLYRALGGGVLTRDEMKAELEKPTGKQQVEEVTNQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=462, Percent_Identity=33.3333333333333, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI2367348, Length=495, Percent_Identity=24.8484848484848, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 57579; Mature: 57447

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNQFNISQFNITQAYIKHKMPIGGVRAFRVNTSVLSVACGILLLSGCAMGPDYQRPDLE
CCCCCCCCEEHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCC
IPEQFHHELQQTDEQNVGLKHWRDFYLDPDLQVLIEHALDQNLDLEGVRSRLLAARAGVT
CCHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCE
VTDAALFPVVGLDANGERALDSALTSTDPVKEDTFYVAGTVSWELDLWGANRRRSEAEFA
EECHHHHCEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHH
NFISSQEALNLATISLISDVASRYYEWLDVEQRYQISLNTVELRKKERDLARLRKQNGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCC
SGLQVRQAEVEYQSARVTLPDLDYERSEKANQLRILLGEFTYPLIPKTEAQLKAEGALFP
CCHHHHHHHHHHHHCEEECCCCCCCHHCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCC
DSFAVGIPSQMLSQRPDVKSAEQALVAANANVGVAKTAFFPKFTISGSYGSESESLSDIF
CCCCCCCCHHHHHCCCCCCHHHHHHEEECCCCCEEEHCCCCEEEECCCCCCCHHHHHHHH
DSQGLTWSLLGGVTAPIFNAGKISAQYDIAQENAKQAMLSYKSTVLGAYFEVNDAMNSFR
HCCCCEEEECCCCHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHH
RSELAIDAQQELVVASTEYTRLATLRYRNGVSSSLDLMDAQRSLFSAELTLSQVKRDRLL
HHHHEECCCCCEEEEECCHHHEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMITLYRALGGGVLTRDEMKAELEKPTGKQQVEEVTNQ
HHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
PNQFNISQFNITQAYIKHKMPIGGVRAFRVNTSVLSVACGILLLSGCAMGPDYQRPDLE
CCCCCCCEEHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCC
IPEQFHHELQQTDEQNVGLKHWRDFYLDPDLQVLIEHALDQNLDLEGVRSRLLAARAGVT
CCHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCE
VTDAALFPVVGLDANGERALDSALTSTDPVKEDTFYVAGTVSWELDLWGANRRRSEAEFA
EECHHHHCEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHH
NFISSQEALNLATISLISDVASRYYEWLDVEQRYQISLNTVELRKKERDLARLRKQNGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCC
SGLQVRQAEVEYQSARVTLPDLDYERSEKANQLRILLGEFTYPLIPKTEAQLKAEGALFP
CCHHHHHHHHHHHHCEEECCCCCCCHHCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCC
DSFAVGIPSQMLSQRPDVKSAEQALVAANANVGVAKTAFFPKFTISGSYGSESESLSDIF
CCCCCCCCHHHHHCCCCCCHHHHHHEEECCCCCEEEHCCCCEEEECCCCCCCHHHHHHHH
DSQGLTWSLLGGVTAPIFNAGKISAQYDIAQENAKQAMLSYKSTVLGAYFEVNDAMNSFR
HCCCCEEEECCCCHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHH
RSELAIDAQQELVVASTEYTRLATLRYRNGVSSSLDLMDAQRSLFSAELTLSQVKRDRLL
HHHHEECCCCCEEEEECCHHHEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMITLYRALGGGVLTRDEMKAELEKPTGKQQVEEVTNQ
HHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]