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The map label for this gene is relA [H]

Identifier: 157961010

GI number: 157961010

Start: 1435005

End: 1437212

Strand: Direct

Name: relA [H]

Synonym: Spea_1182

Alternate gene names: 157961010

Gene position: 1435005-1437212 (Clockwise)

Preceding gene: 157961009

Following gene: 157961011

Centisome position: 27.73

GC content: 46.2

Gene sequence:

>2208_bases
ATGGTATCTGTTCGTGAAGCACATTTTAGCGACCCTGATTTTCAACTTGAAGAATGGGTGCAACGTTATATTAGTGATGC
TTCGGAAGCTCAAATTCTTTTGGCCTTAATTGAGCGGGTCGAACTGCTCGTGGGACAAAAGAAAGACGCGAACGTTACTG
TCTTAAAACGTGCGCGCGAGATGATAGAAATTCTTGCGCCGCTGAATATGGATATTGAAACACTGCAGGCCGCCATCTTG
TTTGTGGTGTTTGATGCTGGGCTCATTACTCAAGAAGATATTGAAGAAGAGTTTGGCGCTAAGCTAGCCGTGTTGGTGTC
GAGTGTTGAGACCATGAACGCTATTGGCGCCCTGAAAGTTGATAATCAGACTCGTAGCGGCGAGCCGCAGATCGATAATA
TTCGTAAGATGTTATTGGCGATGGTGGAAGATGTGCGCGCGGTGGTGATTAAACTTGCCGAACGCATCTGTTTACTTCGT
GAGATTAAAAATGCCGATGAGGAGGTAAAAGTCCTTGTCGCCCGCGAGATTGCCGATATCTATGCGCCACTGGCTAACCG
TCTTGGTATTGGTCAGCTTAAGTGGGAACTAGAGGATATCTCATTCCGTTACCTGCACCCGCAAACTTATAAAGATATCG
CCAAGCAGCTCGATGGTAAGCGCCTCGATAGAGAAACCTTTATCGATGACTTCGTCAGCCAGTTGCAGGCGCGACTCGAT
AAAGATCTGATCCGCGCAAAGGTCTACGGCCGCCCTAAACATATCTACAGTATCTGGAAGAAGATGAAGGGCAAACAGCT
CAAATTTGATGAGCTGTTTGACGTGCGGGCCGTGCGTATCGTGACTGACCGCCTACAAGATTGTTACGGCGCATTAGGCG
TAGTGCATACACTTTGGCACCATATTCCACGTGAATTTGATGACTACGTTGCAAACCCTAAGCCAAACGGTTACCAGTCT
ATTCATACCGTGGTGGTTGGGCCTGAAGATAAAACAGTCGAAATTCAAATTCGCACCGAGCAGATGCATGAAGATGCTGA
GCTTGGGGTTGCGGCGCACTGGAAATATAAAGAAGGTAGCGCCAGCGGTAAACAAAGCGGTTATGAAGAAAAGATTAATT
GGCTGCGCAAAATCTTGCAGTGGCAAGAAGATGTGGCCGAAAGCGGTAACTTAGTCGATGAAGTTCGTAGTCAGGTATTT
GAAGACCGTGTCTATGTGTTTACTCCAAGCGGCGAAGTGGTTGATTTGCCATTAGGCTCAACGGTACTGGATTTTGCTTA
TTACATTCACTCTCATGTGGGTCATAAGTGTATCGGTGCCAAAGTTGATGGCCGCATCGTGCAATTTACTTATCAGGTTG
AGACGGGCGAGCGCATAGAGATCATTACCTCTAAGCATCCTAATCCTAAGCGAGATTGGTTAAATCCCAACTTGGGCTAT
ATTCGTACTTCTCGTGCAAGGTCAAAAATTCTGCACTGGTTTAAACAGCAAGATAGAGATAAAAATATCATTGCTGGGCG
TGAGATGCTTGAAACCGAATTAACCAAGAGTCAGTTGCATATTAAAGATGCGCAATCTGCGGTTGAGCGATTTAATCTAG
TTAGTATGGACGATCTGCTCGCGGGGATTGGTGGTGGTGATATTCGCCTTAACCAAGTGGTAAATCATGTTCAGAGTCGC
CTGCGGACTAAAGAGCTCAGTGATGAAGAGGTGGTTGAAGATTTAGTCAAGAAGAGCCAAAGCAAGTCGGTTAAAAAGGC
CAAAGGCCAAGTTGAGGTTAGCGGCGTTGGTAACTTGATGAGCCATATCGCTAAATGTTGTCAGCCCGTACCAGGCGATG
AAATTTTTGGCTTTATTACTAAAGGCCGCGGTATTTCAGTACACAGAGCCGATTGTGACCAAGTTAAAGAGTTAGTTCGG
GCCTCACCAGAGCGTTCAGTTGAAGTGGTGTGGGGCGAGAACTATTCCGGTGGCTATAAAATTCGCTTAAGTATTTTGGC
TAATGACCGCTCAGGACTGCTGCGAGACTTAACCTCAGTGCTAGCGGCAGAGAAAACCCACGTGATGGCGATGAGTTCGA
CCTCTGATGTGAAAACACAAACCGCCACCATAGAGTTAGAGCTTGAACTATATAATCTAGAGGGTTTATCAAAAGTGTTA
TCCAAGATAAATCAGGTTGATGGGGTCATGCAGGCCAGAAGACTGTAA

Upstream 100 bases:

>100_bases
ACAATAACTGCTGAATGACGAATATAGTGGGTATAAATTAAAAAGATATGGTTAAGCGGCTGTTAACCTCTATCTTGGGT
ATGTAAAGGAATTAAATACG

Downstream 100 bases:

>100_bases
CTTAAAGATAACGGCAACCATAAGGTTGCCGTTTTTATATGTAATTATTAATCGTGACTTTAGCGTGACAGCGTTAAAGC
ACTGTATAGGAAAGTGGAAT

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MVSVREAHFSDPDFQLEEWVQRYISDASEAQILLALIERVELLVGQKKDANVTVLKRAREMIEILAPLNMDIETLQAAIL
FVVFDAGLITQEDIEEEFGAKLAVLVSSVETMNAIGALKVDNQTRSGEPQIDNIRKMLLAMVEDVRAVVIKLAERICLLR
EIKNADEEVKVLVAREIADIYAPLANRLGIGQLKWELEDISFRYLHPQTYKDIAKQLDGKRLDRETFIDDFVSQLQARLD
KDLIRAKVYGRPKHIYSIWKKMKGKQLKFDELFDVRAVRIVTDRLQDCYGALGVVHTLWHHIPREFDDYVANPKPNGYQS
IHTVVVGPEDKTVEIQIRTEQMHEDAELGVAAHWKYKEGSASGKQSGYEEKINWLRKILQWQEDVAESGNLVDEVRSQVF
EDRVYVFTPSGEVVDLPLGSTVLDFAYYIHSHVGHKCIGAKVDGRIVQFTYQVETGERIEIITSKHPNPKRDWLNPNLGY
IRTSRARSKILHWFKQQDRDKNIIAGREMLETELTKSQLHIKDAQSAVERFNLVSMDDLLAGIGGGDIRLNQVVNHVQSR
LRTKELSDEEVVEDLVKKSQSKSVKKAKGQVEVSGVGNLMSHIAKCCQPVPGDEIFGFITKGRGISVHRADCDQVKELVR
ASPERSVEVVWGENYSGGYKIRLSILANDRSGLLRDLTSVLAAEKTHVMAMSSTSDVKTQTATIELELELYNLEGLSKVL
SKINQVDGVMQARRL

Sequences:

>Translated_735_residues
MVSVREAHFSDPDFQLEEWVQRYISDASEAQILLALIERVELLVGQKKDANVTVLKRAREMIEILAPLNMDIETLQAAIL
FVVFDAGLITQEDIEEEFGAKLAVLVSSVETMNAIGALKVDNQTRSGEPQIDNIRKMLLAMVEDVRAVVIKLAERICLLR
EIKNADEEVKVLVAREIADIYAPLANRLGIGQLKWELEDISFRYLHPQTYKDIAKQLDGKRLDRETFIDDFVSQLQARLD
KDLIRAKVYGRPKHIYSIWKKMKGKQLKFDELFDVRAVRIVTDRLQDCYGALGVVHTLWHHIPREFDDYVANPKPNGYQS
IHTVVVGPEDKTVEIQIRTEQMHEDAELGVAAHWKYKEGSASGKQSGYEEKINWLRKILQWQEDVAESGNLVDEVRSQVF
EDRVYVFTPSGEVVDLPLGSTVLDFAYYIHSHVGHKCIGAKVDGRIVQFTYQVETGERIEIITSKHPNPKRDWLNPNLGY
IRTSRARSKILHWFKQQDRDKNIIAGREMLETELTKSQLHIKDAQSAVERFNLVSMDDLLAGIGGGDIRLNQVVNHVQSR
LRTKELSDEEVVEDLVKKSQSKSVKKAKGQVEVSGVGNLMSHIAKCCQPVPGDEIFGFITKGRGISVHRADCDQVKELVR
ASPERSVEVVWGENYSGGYKIRLSILANDRSGLLRDLTSVLAAEKTHVMAMSSTSDVKTQTATIELELELYNLEGLSKVL
SKINQVDGVMQARRL
>Mature_735_residues
MVSVREAHFSDPDFQLEEWVQRYISDASEAQILLALIERVELLVGQKKDANVTVLKRAREMIEILAPLNMDIETLQAAIL
FVVFDAGLITQEDIEEEFGAKLAVLVSSVETMNAIGALKVDNQTRSGEPQIDNIRKMLLAMVEDVRAVVIKLAERICLLR
EIKNADEEVKVLVAREIADIYAPLANRLGIGQLKWELEDISFRYLHPQTYKDIAKQLDGKRLDRETFIDDFVSQLQARLD
KDLIRAKVYGRPKHIYSIWKKMKGKQLKFDELFDVRAVRIVTDRLQDCYGALGVVHTLWHHIPREFDDYVANPKPNGYQS
IHTVVVGPEDKTVEIQIRTEQMHEDAELGVAAHWKYKEGSASGKQSGYEEKINWLRKILQWQEDVAESGNLVDEVRSQVF
EDRVYVFTPSGEVVDLPLGSTVLDFAYYIHSHVGHKCIGAKVDGRIVQFTYQVETGERIEIITSKHPNPKRDWLNPNLGY
IRTSRARSKILHWFKQQDRDKNIIAGREMLETELTKSQLHIKDAQSAVERFNLVSMDDLLAGIGGGDIRLNQVVNHVQSR
LRTKELSDEEVVEDLVKKSQSKSVKKAKGQVEVSGVGNLMSHIAKCCQPVPGDEIFGFITKGRGISVHRADCDQVKELVR
ASPERSVEVVWGENYSGGYKIRLSILANDRSGLLRDLTSVLAAEKTHVMAMSSTSDVKTQTATIELELELYNLEGLSKVL
SKINQVDGVMQARRL

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=741, Percent_Identity=58.5695006747638, Blast_Score=880, Evalue=0.0,
Organism=Escherichia coli, GI1790082, Length=724, Percent_Identity=31.353591160221, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83272; Mature: 83272

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVREAHFSDPDFQLEEWVQRYISDASEAQILLALIERVELLVGQKKDANVTVLKRARE
CCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
MIEILAPLNMDIETLQAAILFVVFDAGLITQEDIEEEFGAKLAVLVSSVETMNAIGALKV
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCEEEE
DNQTRSGEPQIDNIRKMLLAMVEDVRAVVIKLAERICLLREIKNADEEVKVLVAREIADI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YAPLANRLGIGQLKWELEDISFRYLHPQTYKDIAKQLDGKRLDRETFIDDFVSQLQARLD
HHHHHHCCCCCCEEEEEECCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
KDLIRAKVYGRPKHIYSIWKKMKGKQLKFDELFDVRAVRIVTDRLQDCYGALGVVHTLWH
HHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIPREFDDYVANPKPNGYQSIHTVVVGPEDKTVEIQIRTEQMHEDAELGVAAHWKYKEGS
HCCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEEHHHHCCHHHCCEEEEEEECCCC
ASGKQSGYEEKINWLRKILQWQEDVAESGNLVDEVRSQVFEDRVYVFTPSGEVVDLPLGS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCEEECCCCH
TVLDFAYYIHSHVGHKCIGAKVDGRIVQFTYQVETGERIEIITSKHPNPKRDWLNPNLGY
HHHHHHHHHHHHCCCCEECCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCC
IRTSRARSKILHWFKQQDRDKNIIAGREMLETELTKSQLHIKDAQSAVERFNLVSMDDLL
EECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AGIGGGDIRLNQVVNHVQSRLRTKELSDEEVVEDLVKKSQSKSVKKAKGQVEVSGVGNLM
HCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHH
SHIAKCCQPVPGDEIFGFITKGRGISVHRADCDQVKELVRASPERSVEVVWGENYSGGYK
HHHHHHCCCCCCCHHEEEEECCCCCEEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCEE
IRLSILANDRSGLLRDLTSVLAAEKTHVMAMSSTSDVKTQTATIELELELYNLEGLSKVL
EEEEEEECCHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEEEEEEEEECHHHHHHHH
SKINQVDGVMQARRL
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSVREAHFSDPDFQLEEWVQRYISDASEAQILLALIERVELLVGQKKDANVTVLKRARE
CCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
MIEILAPLNMDIETLQAAILFVVFDAGLITQEDIEEEFGAKLAVLVSSVETMNAIGALKV
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCEEEE
DNQTRSGEPQIDNIRKMLLAMVEDVRAVVIKLAERICLLREIKNADEEVKVLVAREIADI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YAPLANRLGIGQLKWELEDISFRYLHPQTYKDIAKQLDGKRLDRETFIDDFVSQLQARLD
HHHHHHCCCCCCEEEEEECCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
KDLIRAKVYGRPKHIYSIWKKMKGKQLKFDELFDVRAVRIVTDRLQDCYGALGVVHTLWH
HHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIPREFDDYVANPKPNGYQSIHTVVVGPEDKTVEIQIRTEQMHEDAELGVAAHWKYKEGS
HCCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEEHHHHCCHHHCCEEEEEEECCCC
ASGKQSGYEEKINWLRKILQWQEDVAESGNLVDEVRSQVFEDRVYVFTPSGEVVDLPLGS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCEEECCCCH
TVLDFAYYIHSHVGHKCIGAKVDGRIVQFTYQVETGERIEIITSKHPNPKRDWLNPNLGY
HHHHHHHHHHHHCCCCEECCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCC
IRTSRARSKILHWFKQQDRDKNIIAGREMLETELTKSQLHIKDAQSAVERFNLVSMDDLL
EECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AGIGGGDIRLNQVVNHVQSRLRTKELSDEEVVEDLVKKSQSKSVKKAKGQVEVSGVGNLM
HCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHH
SHIAKCCQPVPGDEIFGFITKGRGISVHRADCDQVKELVRASPERSVEVVWGENYSGGYK
HHHHHHCCCCCCCHHEEEEECCCCCEEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCEE
IRLSILANDRSGLLRDLTSVLAAEKTHVMAMSSTSDVKTQTATIELELELYNLEGLSKVL
EEEEEEECCHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEEEEEEEEECHHHHHHHH
SKINQVDGVMQARRL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]