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The map label for this gene is rumA [H]

Identifier: 157961009

GI number: 157961009

Start: 1433451

End: 1434788

Strand: Direct

Name: rumA [H]

Synonym: Spea_1181

Alternate gene names: 157961009

Gene position: 1433451-1434788 (Clockwise)

Preceding gene: 157961006

Following gene: 157961010

Centisome position: 27.7

GC content: 46.71

Gene sequence:

>1338_bases
ATGGCACAATTTTTTAAAGCAAAACCAAATAGATCTAAGCAATTATCGTCGAAGTTATCGTTAAAGGTAACGCAGCTGGA
TCACCTCGGGGCTGGCATTGCTCATCACGACGGTAAGATTGTTTTCATCAATGGCGCCTTGCCAGATGAGACGGTATCGG
TGCAGTTAACTGAGCAGAAGAAGAAATTTTCTCGAGGCAAGTTACTCAAAATAGAGAAGCGCTCTACCGAGCGTGTCACT
CCGCCGTGTCCACACTTTGATAAATGTGGTGGTTGCGACCTGCAGCACCTCGACATTGAGGCTCAGCGCAAACATAAGGC
TAATACGCTCGTCGACTTAGTGAATAAATTTGCTCAAACCCAGGCCAGTGAAGTCTGCGCTCCACTCAGCGGTGACGCTT
GGCATTACCGCCGCCGTGCGCGCCTAGCGACCTGGTTCGATAAGAATACTAAGCACATTAGTTTGGGCTTTAGGGCGAGT
AGCAGCAGCGATGTGGTGGAGATCCAGTCTTGTGCGGTATTAGCTGAGCCCTTATCGGCCTTAATTCCAGACTTGGCTTT
TATGCTTAATCAGTTAAGCGGTAAAAAAGCCTTAGGCCACGTTGAGCTGACTCAAGCCGATAACGGCAATTTTGTTGTGC
TACGTGTGACTAAGGTTCTGTCAGATAAAGATAAGGCTCGATTAGTTGAATTTGCGAATAAGCATGAGGTTATTGTGCTG
CTGCAAGATGATGATGCTCAGTGCGAACACCTAAATGGCGCGGGTGAGCAACCATTTTATGCCTTATCGGGCAGTGACAT
TAAGCTTAACTTCTCCCCGGGTAACTTTATTCAAGTAAATGGTCCGGTAAACCAAGCTATGGTGAGCCAGGCTGTTGAAT
GGTTGTCGCCAAAAGCGGGTGAGCGAGTGCTAGATCTTTTCTGTGGTATTGGTAACTTCAGTTTGCCACTTGCCAAAGAT
GGCGCCGAAGTGATTGGTGTCGAAGGGGTTGTGGCTATGGTCGAGCAGGCAAGAGTTAATGCCAAGCAGAGCGGTTTAGA
TAAAGTTGCTTTCTATCATGCCGACTTGAGTGCCGATCTGTCAAAAGAGCCATGGCTAGGCAAGGTCGATAAGATGTTAA
TCGATCCCGCTCGTGCGGGGGCTTATGAGAGCATGCAATCACTGAAAAAGCTAAAACCTAAAGCCTTAGTCTATGTTTCT
TGTAATCCTGCGAGTCTTGCCCGCGACAGTGAAGTGATATTAAAGCAAGGTTACCAGCTTAAAAAAATTGGTTTAATCGA
TATGTTCCCACAGACACATCACTTAGAGTCGATGGCGTTGTTTGAGTTAGGAAATTAA

Upstream 100 bases:

>100_bases
CATTCTTTGTATGGGTTACCATTTAATATAATGTGAGTTATCGATAGACTGCCAGATATTAACCTGTTTTGGCCAGTTTT
TTATCTATTTAGAGAGCAAA

Downstream 100 bases:

>100_bases
GCCAAAAGACACAAACAGATACATTTAATGAAACCTTTTGCTTAAATATAACTCATACACTTCAAACTCAGGCGTTATAA
GCGTTTTTAGTCGCAATTGT

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MAQFFKAKPNRSKQLSSKLSLKVTQLDHLGAGIAHHDGKIVFINGALPDETVSVQLTEQKKKFSRGKLLKIEKRSTERVT
PPCPHFDKCGGCDLQHLDIEAQRKHKANTLVDLVNKFAQTQASEVCAPLSGDAWHYRRRARLATWFDKNTKHISLGFRAS
SSSDVVEIQSCAVLAEPLSALIPDLAFMLNQLSGKKALGHVELTQADNGNFVVLRVTKVLSDKDKARLVEFANKHEVIVL
LQDDDAQCEHLNGAGEQPFYALSGSDIKLNFSPGNFIQVNGPVNQAMVSQAVEWLSPKAGERVLDLFCGIGNFSLPLAKD
GAEVIGVEGVVAMVEQARVNAKQSGLDKVAFYHADLSADLSKEPWLGKVDKMLIDPARAGAYESMQSLKKLKPKALVYVS
CNPASLARDSEVILKQGYQLKKIGLIDMFPQTHHLESMALFELGN

Sequences:

>Translated_445_residues
MAQFFKAKPNRSKQLSSKLSLKVTQLDHLGAGIAHHDGKIVFINGALPDETVSVQLTEQKKKFSRGKLLKIEKRSTERVT
PPCPHFDKCGGCDLQHLDIEAQRKHKANTLVDLVNKFAQTQASEVCAPLSGDAWHYRRRARLATWFDKNTKHISLGFRAS
SSSDVVEIQSCAVLAEPLSALIPDLAFMLNQLSGKKALGHVELTQADNGNFVVLRVTKVLSDKDKARLVEFANKHEVIVL
LQDDDAQCEHLNGAGEQPFYALSGSDIKLNFSPGNFIQVNGPVNQAMVSQAVEWLSPKAGERVLDLFCGIGNFSLPLAKD
GAEVIGVEGVVAMVEQARVNAKQSGLDKVAFYHADLSADLSKEPWLGKVDKMLIDPARAGAYESMQSLKKLKPKALVYVS
CNPASLARDSEVILKQGYQLKKIGLIDMFPQTHHLESMALFELGN
>Mature_444_residues
AQFFKAKPNRSKQLSSKLSLKVTQLDHLGAGIAHHDGKIVFINGALPDETVSVQLTEQKKKFSRGKLLKIEKRSTERVTP
PCPHFDKCGGCDLQHLDIEAQRKHKANTLVDLVNKFAQTQASEVCAPLSGDAWHYRRRARLATWFDKNTKHISLGFRASS
SSDVVEIQSCAVLAEPLSALIPDLAFMLNQLSGKKALGHVELTQADNGNFVVLRVTKVLSDKDKARLVEFANKHEVIVLL
QDDDAQCEHLNGAGEQPFYALSGSDIKLNFSPGNFIQVNGPVNQAMVSQAVEWLSPKAGERVLDLFCGIGNFSLPLAKDG
AEVIGVEGVVAMVEQARVNAKQSGLDKVAFYHADLSADLSKEPWLGKVDKMLIDPARAGAYESMQSLKKLKPKALVYVSC
NPASLARDSEVILKQGYQLKKIGLIDMFPQTHHLESMALFELGN

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=195, Percent_Identity=34.3589743589744, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI51173878, Length=195, Percent_Identity=34.3589743589744, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789148, Length=442, Percent_Identity=45.9276018099548, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1787083, Length=379, Percent_Identity=25.065963060686, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1790403, Length=376, Percent_Identity=25.2659574468085, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24666579, Length=195, Percent_Identity=29.2307692307692, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 48861; Mature: 48730

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFFKAKPNRSKQLSSKLSLKVTQLDHLGAGIAHHDGKIVFINGALPDETVSVQLTEQK
CCCCCCCCCCCHHHHHHHHEEEEEEHHHHCCCEEECCCEEEEEECCCCCCEEEEEEEHHH
KKFSRGKLLKIEKRSTERVTPPCPHFDKCGGCDLQHLDIEAQRKHKANTLVDLVNKFAQT
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
QASEVCAPLSGDAWHYRRRARLATWFDKNTKHISLGFRASSSSDVVEIQSCAVLAEPLSA
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHH
LIPDLAFMLNQLSGKKALGHVELTQADNGNFVVLRVTKVLSDKDKARLVEFANKHEVIVL
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEHHHCCCHHHHHHHHCCCCCEEEE
LQDDDAQCEHLNGAGEQPFYALSGSDIKLNFSPGNFIQVNGPVNQAMVSQAVEWLSPKAG
ECCCCCCHHHCCCCCCCCEEEECCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCCH
ERVLDLFCGIGNFSLPLAKDGAEVIGVEGVVAMVEQARVNAKQSGLDKVAFYHADLSADL
HHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCHHHCCCCEEEEEEECCCCCC
SKEPWLGKVDKMLIDPARAGAYESMQSLKKLKPKALVYVSCNPASLARDSEVILKQGYQL
CCCCCCCHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHCCCEE
KKIGLIDMFPQTHHLESMALFELGN
EEECEEECCCCCCCHHHEEEEECCC
>Mature Secondary Structure 
AQFFKAKPNRSKQLSSKLSLKVTQLDHLGAGIAHHDGKIVFINGALPDETVSVQLTEQK
CCCCCCCCCCHHHHHHHHEEEEEEHHHHCCCEEECCCEEEEEECCCCCCEEEEEEEHHH
KKFSRGKLLKIEKRSTERVTPPCPHFDKCGGCDLQHLDIEAQRKHKANTLVDLVNKFAQT
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
QASEVCAPLSGDAWHYRRRARLATWFDKNTKHISLGFRASSSSDVVEIQSCAVLAEPLSA
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHH
LIPDLAFMLNQLSGKKALGHVELTQADNGNFVVLRVTKVLSDKDKARLVEFANKHEVIVL
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEHHHCCCHHHHHHHHCCCCCEEEE
LQDDDAQCEHLNGAGEQPFYALSGSDIKLNFSPGNFIQVNGPVNQAMVSQAVEWLSPKAG
ECCCCCCHHHCCCCCCCCEEEECCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCCH
ERVLDLFCGIGNFSLPLAKDGAEVIGVEGVVAMVEQARVNAKQSGLDKVAFYHADLSADL
HHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCHHHCCCCEEEEEEECCCCCC
SKEPWLGKVDKMLIDPARAGAYESMQSLKKLKPKALVYVSCNPASLARDSEVILKQGYQL
CCCCCCCHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHCCCEE
KKIGLIDMFPQTHHLESMALFELGN
EEECEEECCCCCCCHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA