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The map label for this gene is yhaJ [H]

Identifier: 157961003

GI number: 157961003

Start: 1423734

End: 1424648

Strand: Direct

Name: yhaJ [H]

Synonym: Spea_1175

Alternate gene names: 157961003

Gene position: 1423734-1424648 (Clockwise)

Preceding gene: 157960999

Following gene: 157961006

Centisome position: 27.51

GC content: 42.4

Gene sequence:

>915_bases
ATGAATACACCTCCGCTTAGTATTGAAGCATTATTGGTCTTAGATGCGATTGAACATCGGGGCAGTTATGCCGCAGCAGC
GGAGCAATTAAATAAAGTCCCTTCAGCACTTTCTTACATAGTGCAAAAGCTAGAAGAACAATTAGGCGTAACATTATTTG
TGCGACAAGGACGGCGAGCGGTGCTTACACCCGCGGGTAAGCATTTATTAGTGGAAGGCCGTAAGGTCTTATCTGTAATC
AATACTTTGTCAGAGCAAACCCAAACCATCGCCAATGGCTGGGAGCCTAAAATTCGCATCGCTTGCGACTCAATTATTGA
TATCAATCCTATTTTCACTGTCATACAGCAATTTTTATCTGAACATCAAAACATTGAGGTGGATGTGCAAGAAGAGGTCA
TGAATGGCACTTGGGAAGCGTTAATTGAAGATAGAGTCGATTTAGTGATTGGTGCCCCAGCTCCGGTACCTAATCAAAAA
GGGATCAGAGCAATAAAGACTGGGGAATTAAACAGTGTATTGGTTGTTAGCAAATCGCATGAGCTGGCAACCGAGGCGTT
GCGCGCTAACGAGCAGACTCAAACAAGCGAACTAAGCAAATATCGAAGCGTGATTGTTCATGATACGGCGAAACATGAAA
TTCCATGGTCAGCTAATTTGCTCGAAGGCAGCCAACATTTTTATGTGAGCTCTATTACACAAAAGATTGCCGCCATTCTT
GCTGGAATAGGAATTGGTCATTTACCAACATCTATGGCCCAGCCATATATTGAGTTAGGCGAGTTGGTTGTGATTGAATT
AGAAAATAAACCCGCACCTCAAGATTTATTTATGGCGTGGAAAATCACAAATAAAGGGAAAGGGTTGAACCGGTTAAAGG
CGATGTTACTAGAGGAAAATAACCCTAATGCTTAA

Upstream 100 bases:

>100_bases
TGTTCGATATGTTCAAAAAATTTGAACAATTAATGGGTGCTATAAGTGAGTCGCTCATTGTCGACATAAAACTTGGCTTA
AAAAAATGAAGGGTTCAACA

Downstream 100 bases:

>100_bases
GTGAGCAAATGATTAAATTCATATGAAATATCATGTATTGCATCTCCCATAGCGTGAATCTCACCACGAAGCGCCACGAC
GTTAGCTGTTAGATACAGTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNTPPLSIEALLVLDAIEHRGSYAAAAEQLNKVPSALSYIVQKLEEQLGVTLFVRQGRRAVLTPAGKHLLVEGRKVLSVI
NTLSEQTQTIANGWEPKIRIACDSIIDINPIFTVIQQFLSEHQNIEVDVQEEVMNGTWEALIEDRVDLVIGAPAPVPNQK
GIRAIKTGELNSVLVVSKSHELATEALRANEQTQTSELSKYRSVIVHDTAKHEIPWSANLLEGSQHFYVSSITQKIAAIL
AGIGIGHLPTSMAQPYIELGELVVIELENKPAPQDLFMAWKITNKGKGLNRLKAMLLEENNPNA

Sequences:

>Translated_304_residues
MNTPPLSIEALLVLDAIEHRGSYAAAAEQLNKVPSALSYIVQKLEEQLGVTLFVRQGRRAVLTPAGKHLLVEGRKVLSVI
NTLSEQTQTIANGWEPKIRIACDSIIDINPIFTVIQQFLSEHQNIEVDVQEEVMNGTWEALIEDRVDLVIGAPAPVPNQK
GIRAIKTGELNSVLVVSKSHELATEALRANEQTQTSELSKYRSVIVHDTAKHEIPWSANLLEGSQHFYVSSITQKIAAIL
AGIGIGHLPTSMAQPYIELGELVVIELENKPAPQDLFMAWKITNKGKGLNRLKAMLLEENNPNA
>Mature_304_residues
MNTPPLSIEALLVLDAIEHRGSYAAAAEQLNKVPSALSYIVQKLEEQLGVTLFVRQGRRAVLTPAGKHLLVEGRKVLSVI
NTLSEQTQTIANGWEPKIRIACDSIIDINPIFTVIQQFLSEHQNIEVDVQEEVMNGTWEALIEDRVDLVIGAPAPVPNQK
GIRAIKTGELNSVLVVSKSHELATEALRANEQTQTSELSKYRSVIVHDTAKHEIPWSANLLEGSQHFYVSSITQKIAAIL
AGIGIGHLPTSMAQPYIELGELVVIELENKPAPQDLFMAWKITNKGKGLNRLKAMLLEENNPNA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789492, Length=283, Percent_Identity=35.6890459363958, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI87082024, Length=270, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787949, Length=286, Percent_Identity=34.6153846153846, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1786713, Length=285, Percent_Identity=28.7719298245614, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786508, Length=284, Percent_Identity=27.112676056338, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI2367136, Length=173, Percent_Identity=30.635838150289, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693105, Length=273, Percent_Identity=24.5421245421245, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790262, Length=180, Percent_Identity=28.3333333333333, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33436; Mature: 33436

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPPLSIEALLVLDAIEHRGSYAAAAEQLNKVPSALSYIVQKLEEQLGVTLFVRQGRRA
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEE
VLTPAGKHLLVEGRKVLSVINTLSEQTQTIANGWEPKIRIACDSIIDINPIFTVIQQFLS
EECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHH
EHQNIEVDVQEEVMNGTWEALIEDRVDLVIGAPAPVPNQKGIRAIKTGELNSVLVVSKSH
HCCCEEEEHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCEEEEEECCH
ELATEALRANEQTQTSELSKYRSVIVHDTAKHEIPWSANLLEGSQHFYVSSITQKIAAIL
HHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGIGIGHLPTSMAQPYIELGELVVIELENKPAPQDLFMAWKITNKGKGLNRLKAMLLEEN
HHCCCCCCCHHHHCHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCC
NPNA
CCCC
>Mature Secondary Structure
MNTPPLSIEALLVLDAIEHRGSYAAAAEQLNKVPSALSYIVQKLEEQLGVTLFVRQGRRA
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEE
VLTPAGKHLLVEGRKVLSVINTLSEQTQTIANGWEPKIRIACDSIIDINPIFTVIQQFLS
EECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHH
EHQNIEVDVQEEVMNGTWEALIEDRVDLVIGAPAPVPNQKGIRAIKTGELNSVLVVSKSH
HCCCEEEEHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCEEEEEECCH
ELATEALRANEQTQTSELSKYRSVIVHDTAKHEIPWSANLLEGSQHFYVSSITQKIAAIL
HHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGIGIGHLPTSMAQPYIELGELVVIELENKPAPQDLFMAWKITNKGKGLNRLKAMLLEEN
HHCCCCCCCHHHHCHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCC
NPNA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]