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The map label for this gene is rstB [H]

Identifier: 157960705

GI number: 157960705

Start: 1075908

End: 1077176

Strand: Reverse

Name: rstB [H]

Synonym: Spea_0877

Alternate gene names: 157960705

Gene position: 1077176-1075908 (Counterclockwise)

Preceding gene: 157960706

Following gene: 157960703

Centisome position: 20.82

GC content: 45.39

Gene sequence:

>1269_bases
ATGAAACGTCTTTTTATTAGCCTCTATCTTCTGCTCAGCCTTGGCTTTTTAGGCATCGGCTGGACTCTGGATAATTTGTG
GCAAAACAATGTTGACGAAAAAGGTGCACTCGATGCACCTTTAGTCGCTTTAGCGCAAGTATTAGCGGCCCTCCCTCCAG
CCGAACGTCAGGCGATGCTAGATTCCATCGAAGCCGACCCGGACTTTGCTCTTACCCTACTCGATTCTGATCAGGTGGCA
TTTTCAAGCGAACAACTCGTTCAAGCCAATAAAGTGATTGCGACCCCCTATGATATCAACCACGAGCTGCAATTTGTGGC
TGTGGGTGAGCAAGTTTTAATGGCTGGCCCTATAGCGATTGACCCTGCAGCCAGTTTAAGAAATATCTACAATATCTTTT
TCTATCTGTTGCTAGCGTTTATTGCACTCATTTGGGTCTGGCCATTATCTAAAGATCTTAAAACCTTACGTAAGGCCACC
AAGGAGTTTGGCCAAGCCAATTGGAACACTCGAATAAAGTTACCCAAACGCTCGCAAGTATTGCCGTTAGCAAATACCTT
TAACGATATGGCTGCTCATATCAGTGCGCTTATCGAAAATCAGAAGCATCTCTCTAATGCCGTTTCACACGAAATCCGCA
CCCCATTAGCGCGGCTTAAATTCGCCTTAGCCCTATTACCTCAGTACTGTCAGCCAAATTCTGATGAGGCAAATCGTCAG
CGCTTTTTAGATGAGATGAAGCTCGATATCAAAGAGATGGAAAACCTGCTACAAGAGTTACTCACATATGCCAGTCTCGA
GACTCAGAGGGAGTCACTCAACTTTGAGCGCTGTGATTTAAACAGCCTAGTCGCACAAACGATAAGACGACTCCAAAGCC
ACAATCCGACTCCTATCGTGCTCGATAAACCAGAGGAAACGATCTACCTAATCGCCGAGCCATCGCTGGTCGAGCGTGCA
CTACAGAATCTCATCACTAACGCTCAAAGGTTCTCGACTGACGATATTAAAGTCAAAATATCTCAAGATACAGACGGGAT
AAGGCTGTCAGTTACCGATCACGGTGAGGGGATTTTAGAGGAAGATCAGAGCAAGATATTTGAGCCATTTTATCGCAGTA
GCAGCAGTAAAAATGGTAATAAGGGTCACGGCTTAGGCTTAGCAATAATCAAGCGGATCATGGACAGACACCATGCCGAG
GTCAGTTTACAAAGCCGGCCTGGCTTTACTCAATTTACCTTATTCTGGCCTAAGACAAAGGAAGATTAA

Upstream 100 bases:

>100_bases
CGATAATATTGAAACGCCATTTAGAATTAAGACGGTATGGGGCCAAGGTTACCTATTCGCACCAGAAGCATGGAATAACT
AAACCAGCGCATTCATAGAG

Downstream 100 bases:

>100_bases
TCTAGCTTAAACTCCTCTAGATTGTCATGTGTCTCTGCCGTTAATTCATCGCCAAATTCAAGACTGGTATTGGCTTGCGC
CCTTGCATCTAATAATTGCG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKRLFISLYLLLSLGFLGIGWTLDNLWQNNVDEKGALDAPLVALAQVLAALPPAERQAMLDSIEADPDFALTLLDSDQVA
FSSEQLVQANKVIATPYDINHELQFVAVGEQVLMAGPIAIDPAASLRNIYNIFFYLLLAFIALIWVWPLSKDLKTLRKAT
KEFGQANWNTRIKLPKRSQVLPLANTFNDMAAHISALIENQKHLSNAVSHEIRTPLARLKFALALLPQYCQPNSDEANRQ
RFLDEMKLDIKEMENLLQELLTYASLETQRESLNFERCDLNSLVAQTIRRLQSHNPTPIVLDKPEETIYLIAEPSLVERA
LQNLITNAQRFSTDDIKVKISQDTDGIRLSVTDHGEGILEEDQSKIFEPFYRSSSSKNGNKGHGLGLAIIKRIMDRHHAE
VSLQSRPGFTQFTLFWPKTKED

Sequences:

>Translated_422_residues
MKRLFISLYLLLSLGFLGIGWTLDNLWQNNVDEKGALDAPLVALAQVLAALPPAERQAMLDSIEADPDFALTLLDSDQVA
FSSEQLVQANKVIATPYDINHELQFVAVGEQVLMAGPIAIDPAASLRNIYNIFFYLLLAFIALIWVWPLSKDLKTLRKAT
KEFGQANWNTRIKLPKRSQVLPLANTFNDMAAHISALIENQKHLSNAVSHEIRTPLARLKFALALLPQYCQPNSDEANRQ
RFLDEMKLDIKEMENLLQELLTYASLETQRESLNFERCDLNSLVAQTIRRLQSHNPTPIVLDKPEETIYLIAEPSLVERA
LQNLITNAQRFSTDDIKVKISQDTDGIRLSVTDHGEGILEEDQSKIFEPFYRSSSSKNGNKGHGLGLAIIKRIMDRHHAE
VSLQSRPGFTQFTLFWPKTKED
>Mature_422_residues
MKRLFISLYLLLSLGFLGIGWTLDNLWQNNVDEKGALDAPLVALAQVLAALPPAERQAMLDSIEADPDFALTLLDSDQVA
FSSEQLVQANKVIATPYDINHELQFVAVGEQVLMAGPIAIDPAASLRNIYNIFFYLLLAFIALIWVWPLSKDLKTLRKAT
KEFGQANWNTRIKLPKRSQVLPLANTFNDMAAHISALIENQKHLSNAVSHEIRTPLARLKFALALLPQYCQPNSDEANRQ
RFLDEMKLDIKEMENLLQELLTYASLETQRESLNFERCDLNSLVAQTIRRLQSHNPTPIVLDKPEETIYLIAEPSLVERA
LQNLITNAQRFSTDDIKVKISQDTDGIRLSVTDHGEGILEEDQSKIFEPFYRSSSSKNGNKGHGLGLAIIKRIMDRHHAE
VSLQSRPGFTQFTLFWPKTKED

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=432, Percent_Identity=28.9351851851852, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1790346, Length=276, Percent_Identity=27.8985507246377, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789808, Length=283, Percent_Identity=27.208480565371, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790551, Length=303, Percent_Identity=27.7227722772277, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790861, Length=207, Percent_Identity=31.8840579710145, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788549, Length=292, Percent_Identity=27.0547945205479, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=28.6995515695067, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786912, Length=215, Percent_Identity=27.906976744186, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI145693157, Length=255, Percent_Identity=25.0980392156863, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=29.8642533936652, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788713, Length=225, Percent_Identity=27.1111111111111, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788393, Length=257, Percent_Identity=21.011673151751, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082128, Length=221, Percent_Identity=26.6968325791855, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786783, Length=310, Percent_Identity=24.5161290322581, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789403, Length=228, Percent_Identity=25.4385964912281, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=274, Percent_Identity=23.3576642335766, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47687; Mature: 47687

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLFISLYLLLSLGFLGIGWTLDNLWQNNVDEKGALDAPLVALAQVLAALPPAERQAML
CHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
DSIEADPDFALTLLDSDQVAFSSEQLVQANKVIATPYDINHELQFVAVGEQVLMAGPIAI
HHCCCCCCEEEEEECCCCCCCCHHHHHHHCCEEECCCCCCCCEEEEEECCHHHEECCCCC
DPAASLRNIYNIFFYLLLAFIALIWVWPLSKDLKTLRKATKEFGQANWNTRIKLPKRSQV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
LPLANTFNDMAAHISALIENQKHLSNAVSHEIRTPLARLKFALALLPQYCQPNSDEANRQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RFLDEMKLDIKEMENLLQELLTYASLETQRESLNFERCDLNSLVAQTIRRLQSHNPTPIV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEE
LDKPEETIYLIAEPSLVERALQNLITNAQRFSTDDIKVKISQDTDGIRLSVTDHGEGILE
EECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCHH
EDQSKIFEPFYRSSSSKNGNKGHGLGLAIIKRIMDRHHAEVSLQSRPGFTQFTLFWPKTK
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCC
ED
CC
>Mature Secondary Structure
MKRLFISLYLLLSLGFLGIGWTLDNLWQNNVDEKGALDAPLVALAQVLAALPPAERQAML
CHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
DSIEADPDFALTLLDSDQVAFSSEQLVQANKVIATPYDINHELQFVAVGEQVLMAGPIAI
HHCCCCCCEEEEEECCCCCCCCHHHHHHHCCEEECCCCCCCCEEEEEECCHHHEECCCCC
DPAASLRNIYNIFFYLLLAFIALIWVWPLSKDLKTLRKATKEFGQANWNTRIKLPKRSQV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
LPLANTFNDMAAHISALIENQKHLSNAVSHEIRTPLARLKFALALLPQYCQPNSDEANRQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RFLDEMKLDIKEMENLLQELLTYASLETQRESLNFERCDLNSLVAQTIRRLQSHNPTPIV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEE
LDKPEETIYLIAEPSLVERALQNLITNAQRFSTDDIKVKISQDTDGIRLSVTDHGEGILE
EECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCHH
EDQSKIFEPFYRSSSSKNGNKGHGLGLAIIKRIMDRHHAEVSLQSRPGFTQFTLFWPKTK
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCC
ED
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]