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The map label for this gene is yfiC [C]

Identifier: 157960631

GI number: 157960631

Start: 994929

End: 995723

Strand: Reverse

Name: yfiC [C]

Synonym: Spea_0803

Alternate gene names: 157960631

Gene position: 995723-994929 (Counterclockwise)

Preceding gene: 157960636

Following gene: 157960630

Centisome position: 19.24

GC content: 48.43

Gene sequence:

>795_bases
ATGCCTTTTACCTTCAAACTCTTTCACGTCGATGATAGCCGCTGCGGTATGCCCGTCAGCACTGACGGTGTACTTCTGGG
TGCTTGGGCGCCATTAGTGCAGGCCAAGACCATACTCGATATTGGTGCTGGCAGTGGCCTATTGAGCCTGATGGCAGCTC
AACGAAGCCTAGCCAAGATTACTGCGATAGAAGTTGATACTGACGCCGCACTAGACTGTCAACAAAACTTCAATGCCTCA
CCTTGGTTCGATCGCCTCGAAGTTATCTGTTGTGACATCCAAGCCTACGCTCAGGCCCACACCCAAGTTCACAGCCAAGC
TCACAATCATGCAGAGCAGAGCAAACAGTTTGAACACATCATCTGTAATCCACCCTATTTTGCCAATGGCCCACAGTCGA
GCAACGTCTCGAGAGCTACGGCGCGCCATACAGACAGCTTAAGTTTCGATAGTCTACTAGCGGCAATAAAACAGCTGTTA
AGCCCTGAAGGCTGTGCCAGCCTTATCTTACCGACAGAATCGGTGAGCCTATTCGAAACTAAACTCAGCACATACCAGCT
AGAACTTAGTCAAAAATTGCTGGCGGCGAGCGTTGAAGGTAAAGAAGCCAATCGACAGATCTTGGTTTTACGCCATACTT
CGGCCTTAGCTCATGACCCAAAAACAGAAACCGAGCCTGCTGTAGTGGCCAAACTTGAAGATGCTGCCCAGCAGCAGCTT
TATATTCGCGAGAAAAACGGCCAATACAGTCAAGCCTTTAGCCTATTGAGTAGAGATTTTTATTTAAAGCTTTAA

Upstream 100 bases:

>100_bases
TTGCATAAAGCATTAACAATTAAACCTAGATTAGAAGATTACGCTATAGCTTTGATTAGCGTACAATTAGCTTCACAGTT
AACACCACCTCATTGTTGCC

Downstream 100 bases:

>100_bases
GGATCTAGCGATCTAATCGTTTAAAGTCGCTTCTATAAGCTTTATAATACTCGGCTATTATTTATTGAGATGCCACGCAT
GCAATTTGAAGATCTCCAGC

Product: methyltransferase small

Products: NA

Alternate protein names: tRNA m6A37 methyltransferase

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MPFTFKLFHVDDSRCGMPVSTDGVLLGAWAPLVQAKTILDIGAGSGLLSLMAAQRSLAKITAIEVDTDAALDCQQNFNAS
PWFDRLEVICCDIQAYAQAHTQVHSQAHNHAEQSKQFEHIICNPPYFANGPQSSNVSRATARHTDSLSFDSLLAAIKQLL
SPEGCASLILPTESVSLFETKLSTYQLELSQKLLAASVEGKEANRQILVLRHTSALAHDPKTETEPAVVAKLEDAAQQQL
YIREKNGQYSQAFSLLSRDFYLKL

Sequences:

>Translated_264_residues
MPFTFKLFHVDDSRCGMPVSTDGVLLGAWAPLVQAKTILDIGAGSGLLSLMAAQRSLAKITAIEVDTDAALDCQQNFNAS
PWFDRLEVICCDIQAYAQAHTQVHSQAHNHAEQSKQFEHIICNPPYFANGPQSSNVSRATARHTDSLSFDSLLAAIKQLL
SPEGCASLILPTESVSLFETKLSTYQLELSQKLLAASVEGKEANRQILVLRHTSALAHDPKTETEPAVVAKLEDAAQQQL
YIREKNGQYSQAFSLLSRDFYLKL
>Mature_263_residues
PFTFKLFHVDDSRCGMPVSTDGVLLGAWAPLVQAKTILDIGAGSGLLSLMAAQRSLAKITAIEVDTDAALDCQQNFNASP
WFDRLEVICCDIQAYAQAHTQVHSQAHNHAEQSKQFEHIICNPPYFANGPQSSNVSRATARHTDSLSFDSLLAAIKQLLS
PEGCASLILPTESVSLFETKLSTYQLELSQKLLAASVEGKEANRQILVLRHTSALAHDPKTETEPAVVAKLEDAAQQQLY
IREKNGQYSQAFSLLSRDFYLKL

Specific function: Specifically methylates the adenine in position 37 of tRNA(Val)

COG id: COG4123

COG function: function code R; Predicted O-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. tRNA (adenine-N(6)-)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI87082131, Length=263, Percent_Identity=37.2623574144487, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMN6_SHEPA (A8H0P3)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001500665.1
- ProteinModelPortal:   A8H0P3
- STRING:   A8H0P3
- GeneID:   5661202
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_0803
- NMPDR:   fig|398579.3.peg.708
- eggNOG:   COG4123
- HOGENOM:   HBG307609
- OMA:   RSKANIT
- ProtClustDB:   CLSK982034
- BioCyc:   SPEA398579:SPEA_0803-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01872
- InterPro:   IPR002052
- InterPro:   IPR020596
- InterPro:   IPR007848
- InterPro:   IPR022882

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.55

Molecular weight: Translated: 28960; Mature: 28829

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00092 N6_MTASE; PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFTFKLFHVDDSRCGMPVSTDGVLLGAWAPLVQAKTILDIGAGSGLLSLMAAQRSLAKI
CCEEEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHE
TAIEVDTDAALDCQQNFNASPWFDRLEVICCDIQAYAQAHTQVHSQAHNHAEQSKQFEHI
EEEEECCCCCCCHHHCCCCCCCHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ICNPPYFANGPQSSNVSRATARHTDSLSFDSLLAAIKQLLSPEGCASLILPTESVSLFET
EECCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
KLSTYQLELSQKLLAASVEGKEANRQILVLRHTSALAHDPKTETEPAVVAKLEDAAQQQL
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHEE
YIREKNGQYSQAFSLLSRDFYLKL
EEEECCCCHHHHHHHHHCCCEEEC
>Mature Secondary Structure 
PFTFKLFHVDDSRCGMPVSTDGVLLGAWAPLVQAKTILDIGAGSGLLSLMAAQRSLAKI
CEEEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHE
TAIEVDTDAALDCQQNFNASPWFDRLEVICCDIQAYAQAHTQVHSQAHNHAEQSKQFEHI
EEEEECCCCCCCHHHCCCCCCCHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ICNPPYFANGPQSSNVSRATARHTDSLSFDSLLAAIKQLLSPEGCASLILPTESVSLFET
EECCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
KLSTYQLELSQKLLAASVEGKEANRQILVLRHTSALAHDPKTETEPAVVAKLEDAAQQQL
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHEE
YIREKNGQYSQAFSLLSRDFYLKL
EEEECCCCHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA