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The map label for this gene is prfA [H]

Identifier: 157825817

GI number: 157825817

Start: 675411

End: 676478

Strand: Direct

Name: prfA [H]

Synonym: A1C_03760

Alternate gene names: 157825817

Gene position: 675411-676478 (Clockwise)

Preceding gene: 157825816

Following gene: 157825818

Centisome position: 54.86

GC content: 37.08

Gene sequence:

>1068_bases
ATGAGTTTTTCAGATAATTTAGCAAAAATTTTAGATAAATATGACAATTTAGGCAAAAAATTATCTTCCGGCATTATGGG
AGACGAATTTGTTAAGGCATCTAAAGAATATGCCGAACTTGAAGATGTTGTAGTAAAAATTAAAGAATATAATAAAGCTA
AATCTGAGCTTGAAGAAGCAAATAATTTTAAGCTAGAACTGGGATTTGATAATGCAACTTTAGCAATGATTGAAGACGAA
ATACATATCCTTGAAAATTCGTTACCAAAACTTGAAAGAGCTGTAAAAATTGCGTTATTACCGAAAGATGATGCGGATAG
TAAAAGTGCTATTATTGAAGTTAGAGCAGGAAGCGGCGGAGAAGAAGCAGCACTTTTTGCTGCCGTACTTTTCAATATGT
ACCAACGATACGCCGAGTTAAAAGGATGGCGTTTCGAGATATTAGCAATTTCCGATACTGGCATAGGCGGTTATAAAGAA
GCATCTGCATCGATAAAAGGCAAAGATGTATTCTCTAAGCTCAAATTTGAGTCGGGCGTTCATAGAGTGCAGAGAGTACC
GGAAACGGAATCACAAGGACGTATTCACACTTCGGCAGCAACAGTTGCAGTACTTCCTGAGGCTGAAGAGGTTGATATTA
AAATTGAAGATAAAGACTTAAGAATTGATACTTATAGAGCTTCAGGGGCTGGTGGACAGCACGTTAATACTACCGATTCT
GCCGTACGTATAACCCATATCCCTACCGGTATTACCGTAGCATTACAAGATGAGAAGTCGCAGCATAAAAATAAAGCAAA
GGCGTTAAAAATTCTACGTGCTAGAATTTATGAAGAAGAACGACGTAATAAGGAACAAGAAAGAGCCGATAGTAGAAGAG
GGCAGATAGGTTCGGGAGATCGTTCGGAACGGATACGTACTTATAATTTCCCACAGGGAAGAGTATCCGACCATAGAATA
AACTTAACACTTTATAAGATAGACGAAGTAGTTAAAAACGGACAATTAGATGAATTTATTGAAGCTTTAATTGCAGAGGA
TGAAGCTAAAAAACTTTCAGAGATATAG

Upstream 100 bases:

>100_bases
CACGGGATCCATAAACACAACTTAAAATACTAATGTTATAGTGTGTTAAGATGGATACCGAGCTCAAGTCGCGGTATGAC
AATAATCAAAATAAAATAAC

Downstream 100 bases:

>100_bases
ATGCGATGTCATGCAATGGCTTGACCACAGCATCCAGTAAAAACCATTTAAAGTATACCAAATTATTTTGGCATTTTTAA
TTGGATTCAGTGATAAATCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSFSDNLAKILDKYDNLGKKLSSGIMGDEFVKASKEYAELEDVVVKIKEYNKAKSELEEANNFKLELGFDNATLAMIEDE
IHILENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGGEEAALFAAVLFNMYQRYAELKGWRFEILAISDTGIGGYKE
ASASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRNKEQERADSRRGQIGSGDRSERIRTYNFPQGRVSDHRI
NLTLYKIDEVVKNGQLDEFIEALIAEDEAKKLSEI

Sequences:

>Translated_355_residues
MSFSDNLAKILDKYDNLGKKLSSGIMGDEFVKASKEYAELEDVVVKIKEYNKAKSELEEANNFKLELGFDNATLAMIEDE
IHILENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGGEEAALFAAVLFNMYQRYAELKGWRFEILAISDTGIGGYKE
ASASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRNKEQERADSRRGQIGSGDRSERIRTYNFPQGRVSDHRI
NLTLYKIDEVVKNGQLDEFIEALIAEDEAKKLSEI
>Mature_354_residues
SFSDNLAKILDKYDNLGKKLSSGIMGDEFVKASKEYAELEDVVVKIKEYNKAKSELEEANNFKLELGFDNATLAMIEDEI
HILENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGGEEAALFAAVLFNMYQRYAELKGWRFEILAISDTGIGGYKEA
SASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDSA
VRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRNKEQERADSRRGQIGSGDRSERIRTYNFPQGRVSDHRIN
LTLYKIDEVVKNGQLDEFIEALIAEDEAKKLSEI

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=315, Percent_Identity=46.984126984127, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI34577120, Length=369, Percent_Identity=38.7533875338753, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI166795305, Length=208, Percent_Identity=49.0384615384615, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1787462, Length=328, Percent_Identity=54.5731707317073, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI2367172, Length=314, Percent_Identity=39.4904458598726, Blast_Score=195, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=261, Percent_Identity=42.1455938697318, Blast_Score=198, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=370, Percent_Identity=42.4324324324324, Blast_Score=245, Evalue=8e-66,
Organism=Drosophila melanogaster, GI19921226, Length=328, Percent_Identity=42.9878048780488, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39702; Mature: 39571

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSDNLAKILDKYDNLGKKLSSGIMGDEFVKASKEYAELEDVVVKIKEYNKAKSELEEA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNFKLELGFDNATLAMIEDEIHILENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGG
CCEEEEECCCCCEEEEEHHHHHHHHCCHHHHHHHEEEEEECCCCCCCCEEEEEEECCCCC
EEAALFAAVLFNMYQRYAELKGWRFEILAISDTGIGGYKEASASIKGKDVFSKLKFESGV
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHH
HRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRNKEQERADSRRGQIGSGD
EEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCC
RSERIRTYNFPQGRVSDHRINLTLYKIDEVVKNGQLDEFIEALIAEDEAKKLSEI
CCCCCEEECCCCCCCCCCEEEEEEEEHHHHHHCCCHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
SFSDNLAKILDKYDNLGKKLSSGIMGDEFVKASKEYAELEDVVVKIKEYNKAKSELEEA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNFKLELGFDNATLAMIEDEIHILENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGG
CCEEEEECCCCCEEEEEHHHHHHHHCCHHHHHHHEEEEEECCCCCCCCEEEEEEECCCCC
EEAALFAAVLFNMYQRYAELKGWRFEILAISDTGIGGYKEASASIKGKDVFSKLKFESGV
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHH
HRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRNKEQERADSRRGQIGSGD
EEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCC
RSERIRTYNFPQGRVSDHRINLTLYKIDEVVKNGQLDEFIEALIAEDEAKKLSEI
CCCCCEEECCCCCCCCCCEEEEEEEEHHHHHHCCCHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA