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The map label for this gene is pdhC [H]

Identifier: 157825816

GI number: 157825816

Start: 674008

End: 675246

Strand: Direct

Name: pdhC [H]

Synonym: A1C_03755

Alternate gene names: 157825816

Gene position: 674008-675246 (Clockwise)

Preceding gene: 157825815

Following gene: 157825817

Centisome position: 54.75

GC content: 35.67

Gene sequence:

>1239_bases
ATGCCGATTAAAATTTTAATGCCTGCTTTATCTCCGACTATGACGGCAGGCAATCTAGCTAGGTGGTTAAAGAAAGAAGG
GGATAAAGTTAATCCTGGGGAAGTGATTGCCGAAATTGAAACCGATAAAGCCACGATGGAAGTAGAAGCAGTAGATGAAG
GTATACTTGCCAAAATAGTTATCCCACAAAATAGTCAAAATGTACCTGTTAACTCTTTAATTGCCGTATTAAGTGAAGCA
AGAGAAGAAAAAGCGGATATTGATGCGTTTATTGCAAAAAATAATAATGTATCACCGTCACCGAAACCAGATACTAACCT
TCCAAAACACCACGAGAATATAGCTAAGGTAGAAGAACAGGTAGCAGTGATAAAACATGATACTAGTAAAATATTCGCCT
CACCTCTTGCAAAAAGACTTGCAAAAATGGGAAATATTAAACTTGAGAGCGTGAAAGGTAGTGGTCCGCACGGTAGAATA
GTCAAACAAGATGTCTTATCATATACTCCTAGCACTGTTCATAATAAAATAGTTAGTAGAAATCCTGAAGAATATCGTTT
AGTACCAAATAATAATATCCGCAAGATTATAGCCAAGCGTCTGCTTGAATCTAAACAAACAGTTCCGCATTTTTATTTAT
CTATAGAATGTAATGTTGATAAATTGTTAGATATAAGAGAAGATATTAATAAATCTTTTTCTGAAGATAAAGCAACAAGG
ATATCGGTTAATGATTTTATTATTTTAGCAGTAGCCAAAGCTTTACAAGCAGTACCAAATGCCAATGCTAGTTGGAGGGA
AGATGCGATTAGATATTACAATAATGTCGATATTTCAGTAGCGGTAGCGATTGAGAACGGGCTTGTTACACCGATAGTTA
AAAATGCCAATCAAAAAAACATTATAGAGATATCTCGTGAAATGAAAAAATTAATAAAGAAAGCAAAAGATAACAAATTA
ACCCCTGAAGAATTTCAGGGCGGAGGGTTTACAATTTCTAACCTCGGTATGTACGGTGTGAAAAATTTCAATGCTATAAT
TAACCCACCGCAAAGTTGTATAATGGGCGTCGGAGCCAGTGCAAAACGTGCTATAGTCAAGAATGATCAAATAACTATAG
AAACAATTATGGATGTAACTCTTTCTGCAGATCACCGAGTAGTAGACGGAGCAGTCGGTGCTGAGTTCTTGGCAGCATTT
AAGAAATTTATAGAAAGCCCAGCTTTGATGTTGATATAA

Upstream 100 bases:

>100_bases
TTCCTACCTACGCAGGAATGACATAGAAGAGCCATACAACAACGCCTCCTCGCAATAATGTTAAGAACAACAATAATAAA
AAATCGAATCTAAACAAATT

Downstream 100 bases:

>100_bases
GTCGTCATTGCGAGCATTGCACCTGTGGATCAATTTCAGCTCTGTGTCATCCTGTGGCCTTGACCACGGGATCCATAAAC
ACAACTTAAAATACTAATGT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPIKILMPALSPTMTAGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIVIPQNSQNVPVNSLIAVLSEA
REEKADIDAFIAKNNNVSPSPKPDTNLPKHHENIAKVEEQVAVIKHDTSKIFASPLAKRLAKMGNIKLESVKGSGPHGRI
VKQDVLSYTPSTVHNKIVSRNPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKSFSEDKATR
ISVNDFIILAVAKALQAVPNANASWREDAIRYYNNVDISVAVAIENGLVTPIVKNANQKNIIEISREMKKLIKKAKDNKL
TPEEFQGGGFTISNLGMYGVKNFNAIINPPQSCIMGVGASAKRAIVKNDQITIETIMDVTLSADHRVVDGAVGAEFLAAF
KKFIESPALMLI

Sequences:

>Translated_412_residues
MPIKILMPALSPTMTAGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIVIPQNSQNVPVNSLIAVLSEA
REEKADIDAFIAKNNNVSPSPKPDTNLPKHHENIAKVEEQVAVIKHDTSKIFASPLAKRLAKMGNIKLESVKGSGPHGRI
VKQDVLSYTPSTVHNKIVSRNPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKSFSEDKATR
ISVNDFIILAVAKALQAVPNANASWREDAIRYYNNVDISVAVAIENGLVTPIVKNANQKNIIEISREMKKLIKKAKDNKL
TPEEFQGGGFTISNLGMYGVKNFNAIINPPQSCIMGVGASAKRAIVKNDQITIETIMDVTLSADHRVVDGAVGAEFLAAF
KKFIESPALMLI
>Mature_411_residues
PIKILMPALSPTMTAGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIVIPQNSQNVPVNSLIAVLSEAR
EEKADIDAFIAKNNNVSPSPKPDTNLPKHHENIAKVEEQVAVIKHDTSKIFASPLAKRLAKMGNIKLESVKGSGPHGRIV
KQDVLSYTPSTVHNKIVSRNPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKSFSEDKATRI
SVNDFIILAVAKALQAVPNANASWREDAIRYYNNVDISVAVAIENGLVTPIVKNANQKNIIEISREMKKLIKKAKDNKLT
PEEFQGGGFTISNLGMYGVKNFNAIINPPQSCIMGVGASAKRAIVKNDQITIETIMDVTLSADHRVVDGAVGAEFLAAFK
KFIESPALMLI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=447, Percent_Identity=42.2818791946309, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI203098753, Length=457, Percent_Identity=38.074398249453, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI203098816, Length=457, Percent_Identity=38.074398249453, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=27.9620853080569, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI260898739, Length=164, Percent_Identity=45.7317073170732, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI19923748, Length=224, Percent_Identity=33.0357142857143, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1786946, Length=420, Percent_Identity=31.4285714285714, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1786305, Length=395, Percent_Identity=31.3924050632911, Blast_Score=161, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=434, Percent_Identity=42.1658986175115, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=35.8306188925081, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=418, Percent_Identity=29.6650717703349, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=412, Percent_Identity=28.8834951456311, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=40.4867256637168, Blast_Score=307, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6320352, Length=412, Percent_Identity=30.0970873786408, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321632, Length=174, Percent_Identity=31.6091954022989, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20129315, Length=293, Percent_Identity=46.4163822525597, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24582497, Length=293, Percent_Identity=46.4163822525597, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=26.3157894736842, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=32.6446280991736, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45294; Mature: 45163

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00178 AA_TRNA_LIGASE_I ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIKILMPALSPTMTAGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIV
CCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IPQNSQNVPVNSLIAVLSEAREEKADIDAFIAKNNNVSPSPKPDTNLPKHHENIAKVEEQ
ECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
VAVIKHDTSKIFASPLAKRLAKMGNIKLESVKGSGPHGRIVKQDVLSYTPSTVHNKIVSR
HHHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHC
NPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKSFSEDKATR
CCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCEE
ISVNDFIILAVAKALQAVPNANASWREDAIRYYNNVDISVAVAIENGLVTPIVKNANQKN
EECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCH
IIEISREMKKLIKKAKDNKLTPEEFQGGGFTISNLGMYGVKNFNAIINPPQSCIMGVGAS
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHCCCCC
AKRAIVKNDQITIETIMDVTLSADHRVVDGAVGAEFLAAFKKFIESPALMLI
CCHHEECCCCEEEEEHHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
PIKILMPALSPTMTAGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIV
CCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IPQNSQNVPVNSLIAVLSEAREEKADIDAFIAKNNNVSPSPKPDTNLPKHHENIAKVEEQ
ECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
VAVIKHDTSKIFASPLAKRLAKMGNIKLESVKGSGPHGRIVKQDVLSYTPSTVHNKIVSR
HHHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHC
NPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKSFSEDKATR
CCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCEE
ISVNDFIILAVAKALQAVPNANASWREDAIRYYNNVDISVAVAIENGLVTPIVKNANQKN
EECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCH
IIEISREMKKLIKKAKDNKLTPEEFQGGGFTISNLGMYGVKNFNAIINPPQSCIMGVGAS
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHCCCCC
AKRAIVKNDQITIETIMDVTLSADHRVVDGAVGAEFLAAFKKFIESPALMLI
CCHHEECCCCEEEEEHHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]