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The map label for this gene is mdh [H]

Identifier: 157825645

GI number: 157825645

Start: 511645

End: 512589

Strand: Reverse

Name: mdh [H]

Synonym: A1C_02825

Alternate gene names: 157825645

Gene position: 512589-511645 (Counterclockwise)

Preceding gene: 157825646

Following gene: 157825644

Centisome position: 41.64

GC content: 36.93

Gene sequence:

>945_bases
ATGAAAAAAAATCCAAAAATCTCATTAATAGGTAGCGGTAACATAGGTGGTACGCTTGCTCACCTAATTAGCCTTAGAGA
ACTCGGTGATATCGTATTATTTGATGTTGCTGAAGGAGTACCGCAAGGAAAAGCATTGGATCTCATGCAAGCCAGTACAA
TAGCCGGGTCTGATATCAAAATAACAGGTACAAACGACTATAAAGATATTGAAGGGTCTGATGCAATAATTATTACTGCC
GGTTTACCAAGGAAACCAGGGATGAGTAGAGACGATTTAATTAGTATTAATACCGGTATTATGAAAAATGTTGCAGAGAA
CGTTAAAAAATACGCTCCTGATGCTTTTGTAATAGTAATTACCAATCCGCTTGATGTTATGGTATATGTCATGCTTAAAG
CAAGCGGATTACCTCATAATCAAGTCATAGGTATGGCAGGCGTACTTGACTCATCAAGATTTAATCTTTTTCTTGCCGAA
GAGTTTAAAGTATCGGTGAGCAATGTTAATAGCACGGTTCTCGGCGGTCATGGTGATGCTATGGTACCTCTTGCAAGATA
TTCTACAATATCAGGCATACCGATACCTGACTTGATAAAAATGGGCTTATCAAGTAATGAAAATATTGAAAAAATAATCG
ATCGTACTAGGAACGGCGGCGGCGATATTGTTGCATTACTTAAAACAGGTTCTGCTTATTATGCACCCGCAGCCTCAGCA
ATAGAAATGCTTGAAGCATATTTAAAAGATAAACGTCAAATGCTAACTTGTGCCGCTTATCTACAAGGTGAATATGGGGT
TCATGATTTATATGTCGGAGTACCTATTATTATAGGTAAAGAAGGTGTCATAAAAGTAGTAGAACTACAATTAACTAAAG
AAGAAAAAACATTATTCGACAAATCAGTTGAGGGAGTTAAAACACTCATTGAGACGATACAATAA

Upstream 100 bases:

>100_bases
TTTTCGATTCGGCTTTTAATAATGTCAATTTAAAAATTCTTTTTTCTTGAATCTATTTGTAATATAGTAACAGGCATATT
TAATATTTGTAGAGGGAGTA

Downstream 100 bases:

>100_bases
AATTGTTAATTTAACATAAATGGTATTACATTTTAGTATATTTTTATTAATAGCAGGAAACTATGAAACATAAGAATGAA
TTAGATCAGCTAATAAGAAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKNPKISLIGSGNIGGTLAHLISLRELGDIVLFDVAEGVPQGKALDLMQASTIAGSDIKITGTNDYKDIEGSDAIIITA
GLPRKPGMSRDDLISINTGIMKNVAENVKKYAPDAFVIVITNPLDVMVYVMLKASGLPHNQVIGMAGVLDSSRFNLFLAE
EFKVSVSNVNSTVLGGHGDAMVPLARYSTISGIPIPDLIKMGLSSNENIEKIIDRTRNGGGDIVALLKTGSAYYAPAASA
IEMLEAYLKDKRQMLTCAAYLQGEYGVHDLYVGVPIIIGKEGVIKVVELQLTKEEKTLFDKSVEGVKTLIETIQ

Sequences:

>Translated_314_residues
MKKNPKISLIGSGNIGGTLAHLISLRELGDIVLFDVAEGVPQGKALDLMQASTIAGSDIKITGTNDYKDIEGSDAIIITA
GLPRKPGMSRDDLISINTGIMKNVAENVKKYAPDAFVIVITNPLDVMVYVMLKASGLPHNQVIGMAGVLDSSRFNLFLAE
EFKVSVSNVNSTVLGGHGDAMVPLARYSTISGIPIPDLIKMGLSSNENIEKIIDRTRNGGGDIVALLKTGSAYYAPAASA
IEMLEAYLKDKRQMLTCAAYLQGEYGVHDLYVGVPIIIGKEGVIKVVELQLTKEEKTLFDKSVEGVKTLIETIQ
>Mature_314_residues
MKKNPKISLIGSGNIGGTLAHLISLRELGDIVLFDVAEGVPQGKALDLMQASTIAGSDIKITGTNDYKDIEGSDAIIITA
GLPRKPGMSRDDLISINTGIMKNVAENVKKYAPDAFVIVITNPLDVMVYVMLKASGLPHNQVIGMAGVLDSSRFNLFLAE
EFKVSVSNVNSTVLGGHGDAMVPLARYSTISGIPIPDLIKMGLSSNENIEKIIDRTRNGGGDIVALLKTGSAYYAPAASA
IEMLEAYLKDKRQMLTCAAYLQGEYGVHDLYVGVPIIIGKEGVIKVVELQLTKEEKTLFDKSVEGVKTLIETIQ

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=303, Percent_Identity=33.003300330033, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI221136809, Length=303, Percent_Identity=33.003300330033, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI9257228, Length=303, Percent_Identity=32.3432343234323, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI4504973, Length=303, Percent_Identity=32.3432343234323, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=30.6962025316456, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=30.6962025316456, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI15082234, Length=315, Percent_Identity=28.8888888888889, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=29.1803278688525, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=29.1803278688525, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI260099727, Length=198, Percent_Identity=35.8585858585859, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI260099725, Length=198, Percent_Identity=35.8585858585859, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI21735621, Length=297, Percent_Identity=29.2929292929293, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI207028494, Length=202, Percent_Identity=27.7227722772277, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI103472011, Length=294, Percent_Identity=22.108843537415, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1789632, Length=291, Percent_Identity=31.6151202749141, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17535107, Length=272, Percent_Identity=27.9411764705882, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17554310, Length=309, Percent_Identity=29.4498381877023, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=301, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=318, Percent_Identity=26.7295597484277, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324446, Length=255, Percent_Identity=27.843137254902, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17136226, Length=300, Percent_Identity=30, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI45550422, Length=299, Percent_Identity=29.0969899665552, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647881, Length=323, Percent_Identity=29.7213622291022, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24663599, Length=291, Percent_Identity=29.553264604811, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24663595, Length=283, Percent_Identity=26.5017667844523, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33608; Mature: 33608

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNPKISLIGSGNIGGTLAHLISLRELGDIVLFDVAEGVPQGKALDLMQASTIAGSDIK
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHEECCCCEE
ITGTNDYKDIEGSDAIIITAGLPRKPGMSRDDLISINTGIMKNVAENVKKYAPDAFVIVI
EECCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEE
TNPLDVMVYVMLKASGLPHNQVIGMAGVLDSSRFNLFLAEEFKVSVSNVNSTVLGGHGDA
ECHHHHHHHHHHHCCCCCCHHHEEEHHEECCCCEEEEEEEEEEEEEECCCEEEECCCCCC
MVPLARYSTISGIPIPDLIKMGLSSNENIEKIIDRTRNGGGDIVALLKTGSAYYAPAASA
EEEHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEEECCCEEECCHHHH
IEMLEAYLKDKRQMLTCAAYLQGEYGVHDLYVGVPIIIGKEGVIKVVELQLTKEEKTLFD
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCEEEEEEEECCHHHHHHH
KSVEGVKTLIETIQ
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKKNPKISLIGSGNIGGTLAHLISLRELGDIVLFDVAEGVPQGKALDLMQASTIAGSDIK
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHEECCCCEE
ITGTNDYKDIEGSDAIIITAGLPRKPGMSRDDLISINTGIMKNVAENVKKYAPDAFVIVI
EECCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEE
TNPLDVMVYVMLKASGLPHNQVIGMAGVLDSSRFNLFLAEEFKVSVSNVNSTVLGGHGDA
ECHHHHHHHHHHHCCCCCCHHHEEEHHEECCCCEEEEEEEEEEEEEECCCEEEECCCCCC
MVPLARYSTISGIPIPDLIKMGLSSNENIEKIIDRTRNGGGDIVALLKTGSAYYAPAASA
EEEHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEEECCCEEECCHHHH
IEMLEAYLKDKRQMLTCAAYLQGEYGVHDLYVGVPIIIGKEGVIKVVELQLTKEEKTLFD
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCEEEEEEEECCHHHHHHH
KSVEGVKTLIETIQ
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA