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The map label for this gene is pleD [H]

Identifier: 157825448

GI number: 157825448

Start: 327160

End: 328512

Strand: Reverse

Name: pleD [H]

Synonym: A1C_01745

Alternate gene names: 157825448

Gene position: 328512-327160 (Counterclockwise)

Preceding gene: 157825456

Following gene: 157825447

Centisome position: 26.69

GC content: 31.56

Gene sequence:

>1353_bases
ATGACCACAATATTAGTAGTAGATGATATAGAAACCAATATTAAGTTACTGACAGCCAAACTTTTAAAAGAGTATTATAC
GGTTCTTACTGCAAATAGCGGCAAAGAAGCATTAGCAATTCTTAAAAAGGAAAAAATCGATACTGTACTACTTGATGTTA
TGATGCCGGAAATGGATGGATTTGAGGTATGTAAAACGATAAAAACCAATCCGGAAACTACTCATGTACCAGTCGTAATG
GTAACTGCACTTTCTGATGTTGATGATCGAGTTAAAGGTCTTGAGGCAGGGGCTGATGAGTTTTTAACAAAGCCGATTAA
CGATACTGCTCTATTTGTAAGACTTAAATCGCTCTCTAGAATGAAAAGCTTAATTGATGAATTAAAGCTTCGTAATAGCA
CTAATGCATTATTAGGCGTAACAAATATCGAAATGCATGACACTTTTGCAGATAAAAAAATACTACTAATTAATGATGAT
GTGATACAAGCTAAAAATATAAAACAAATGTTACTTAAGATTACACAAAACGTAAAGGTAATAAGTAATTCCGATGAATT
AGATATTATAAATGAATATACACCAGATTTAGTAATTATCAGTAGTACGCTCGAAAATGAAGATCCTTTGAGAATCAGTG
TTATTTTAAGAGGTAAAGAAGAAATAAGAGGCGTTGTAATAATTTTACAAATTGATGAAGACGGTATGCCTTTAGTTGTG
AAGGGTATTGAACTCGGTGTTAATGATTATTTCGTTTATCCTATAGAGGAAAGCGAACTACTTGCCAGAATTAGAACACA
ATTAAGGCGTAAGCAATATCAAGATAATTTACATAATGATCTTGAACAAAGTGTTAATTTAGCAGCTAAAGATGGCTTAA
CCGGCTTATTTAATCGTCGCTATTTCGATATACACCTTAAACAAATGATTGAGAAAGCTAATAATGAAAGTATTAAATTA
TATTTACTGATGTGCGATATCGACAATTTTAAACACGTAAACGATACTTACGGTCATCAAGCAGGTGATAAGGTTTTAAC
GATTGTATCCCGTATTTTGAAGAATACGCTTAGAGTAACAGACTTAATAGCAAGATTCGGCGGTGAAGAGTTTACTATAC
TTTTAACGGATATAGATATTTCTAAAGCAATTGAAACTGCGGAGAGAATTAGAGTTAAAATAGAATATATGGATTTTCAT
ATTGAAGATCAAATTGAACCTTTACAAAAAACTATTTCAATCGGAGTTACAGAATATAAAAAAGCAGAATCAATAGAATC
TTTCATTGAACGTGCTGACAAAGCTATGTATGAAGCTAAAACAACGGGTAGAAATAAAGTAGTAAAATTATAA

Upstream 100 bases:

>100_bases
TAATTGAATTTGCTGAAATTTTCATATATTATACATTTGAGACTTAAAATTAAAGTATAAATATACAAATTATTTCATTC
AAAAACAAGAATTTAATCTT

Downstream 100 bases:

>100_bases
TTGATTTTTTAAAGTAAAAAATAATGAGAAATTCTTCCAAAAAACATCTTGATTTACCATATAGACTAGGAGTCGGCATG
ATGATATTAAATGCCGATAA

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTTILVVDDIETNIKLLTAKLLKEYYTVLTANSGKEALAILKKEKIDTVLLDVMMPEMDGFEVCKTIKTNPETTHVPVVM
VTALSDVDDRVKGLEAGADEFLTKPINDTALFVRLKSLSRMKSLIDELKLRNSTNALLGVTNIEMHDTFADKKILLINDD
VIQAKNIKQMLLKITQNVKVISNSDELDIINEYTPDLVIISSTLENEDPLRISVILRGKEEIRGVVIILQIDEDGMPLVV
KGIELGVNDYFVYPIEESELLARIRTQLRRKQYQDNLHNDLEQSVNLAAKDGLTGLFNRRYFDIHLKQMIEKANNESIKL
YLLMCDIDNFKHVNDTYGHQAGDKVLTIVSRILKNTLRVTDLIARFGGEEFTILLTDIDISKAIETAERIRVKIEYMDFH
IEDQIEPLQKTISIGVTEYKKAESIESFIERADKAMYEAKTTGRNKVVKL

Sequences:

>Translated_450_residues
MTTILVVDDIETNIKLLTAKLLKEYYTVLTANSGKEALAILKKEKIDTVLLDVMMPEMDGFEVCKTIKTNPETTHVPVVM
VTALSDVDDRVKGLEAGADEFLTKPINDTALFVRLKSLSRMKSLIDELKLRNSTNALLGVTNIEMHDTFADKKILLINDD
VIQAKNIKQMLLKITQNVKVISNSDELDIINEYTPDLVIISSTLENEDPLRISVILRGKEEIRGVVIILQIDEDGMPLVV
KGIELGVNDYFVYPIEESELLARIRTQLRRKQYQDNLHNDLEQSVNLAAKDGLTGLFNRRYFDIHLKQMIEKANNESIKL
YLLMCDIDNFKHVNDTYGHQAGDKVLTIVSRILKNTLRVTDLIARFGGEEFTILLTDIDISKAIETAERIRVKIEYMDFH
IEDQIEPLQKTISIGVTEYKKAESIESFIERADKAMYEAKTTGRNKVVKL
>Mature_449_residues
TTILVVDDIETNIKLLTAKLLKEYYTVLTANSGKEALAILKKEKIDTVLLDVMMPEMDGFEVCKTIKTNPETTHVPVVMV
TALSDVDDRVKGLEAGADEFLTKPINDTALFVRLKSLSRMKSLIDELKLRNSTNALLGVTNIEMHDTFADKKILLINDDV
IQAKNIKQMLLKITQNVKVISNSDELDIINEYTPDLVIISSTLENEDPLRISVILRGKEEIRGVVIILQIDEDGMPLVVK
GIELGVNDYFVYPIEESELLARIRTQLRRKQYQDNLHNDLEQSVNLAAKDGLTGLFNRRYFDIHLKQMIEKANNESIKLY
LLMCDIDNFKHVNDTYGHQAGDKVLTIVSRILKNTLRVTDLIARFGGEEFTILLTDIDISKAIETAERIRVKIEYMDFHI
EDQIEPLQKTISIGVTEYKKAESIESFIERADKAMYEAKTTGRNKVVKL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=177, Percent_Identity=41.8079096045198, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=40.2366863905325, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=38.2165605095541, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=32.9192546583851, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788085, Length=174, Percent_Identity=35.0574712643678, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=38.3647798742138, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=32.7160493827161, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=32.5, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=178, Percent_Identity=31.4606741573034, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082012, Length=142, Percent_Identity=28.8732394366197, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081974, Length=162, Percent_Identity=30.2469135802469, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787056, Length=168, Percent_Identity=31.547619047619, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=163, Percent_Identity=34.3558282208589, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789809, Length=141, Percent_Identity=30.4964539007092, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788956, Length=179, Percent_Identity=29.608938547486, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790437, Length=105, Percent_Identity=42.8571428571429, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=42.7184466019417, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=124, Percent_Identity=34.6774193548387, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=36.4406779661017, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788905, Length=110, Percent_Identity=40.9090909090909, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788394, Length=135, Percent_Identity=31.1111111111111, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786784, Length=122, Percent_Identity=28.6885245901639, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787229, Length=115, Percent_Identity=33.9130434782609, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788550, Length=139, Percent_Identity=32.3741007194245, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367329, Length=120, Percent_Identity=38.3333333333333, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081977, Length=151, Percent_Identity=31.1258278145695, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786911, Length=123, Percent_Identity=30.0813008130081, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790860, Length=119, Percent_Identity=31.9327731092437, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51147; Mature: 51016

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTILVVDDIETNIKLLTAKLLKEYYTVLTANSGKEALAILKKEKIDTVLLDVMMPEMDG
CEEEEEEECCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
FEVCKTIKTNPETTHVPVVMVTALSDVDDRVKGLEAGADEFLTKPINDTALFVRLKSLSR
HHHHHHHCCCCCCCCCCEEEEEEHHCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHH
MKSLIDELKLRNSTNALLGVTNIEMHDTFADKKILLINDDVIQAKNIKQMLLKITQNVKV
HHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEE
ISNSDELDIINEYTPDLVIISSTLENEDPLRISVILRGKEEIRGVVIILQIDEDGMPLVV
EECCCCEEEHHCCCCCEEEEEECCCCCCCEEEEEEEECHHHCCEEEEEEEECCCCCCEEE
KGIELGVNDYFVYPIEESELLARIRTQLRRKQYQDNLHNDLEQSVNLAAKDGLTGLFNRR
EEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHCCE
YFDIHLKQMIEKANNESIKLYLLMCDIDNFKHVNDTYGHQAGDKVLTIVSRILKNTLRVT
EEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DLIARFGGEEFTILLTDIDISKAIETAERIRVKIEYMDFHIEDQIEPLQKTISIGVTEYK
HHHHHHCCCEEEEEEEECCHHHHHHHHHHEEEEEEEEEEEEHHHHHHHHHHHHCCCHHHH
KAESIESFIERADKAMYEAKTTGRNKVVKL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
>Mature Secondary Structure 
TTILVVDDIETNIKLLTAKLLKEYYTVLTANSGKEALAILKKEKIDTVLLDVMMPEMDG
EEEEEEECCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
FEVCKTIKTNPETTHVPVVMVTALSDVDDRVKGLEAGADEFLTKPINDTALFVRLKSLSR
HHHHHHHCCCCCCCCCCEEEEEEHHCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHH
MKSLIDELKLRNSTNALLGVTNIEMHDTFADKKILLINDDVIQAKNIKQMLLKITQNVKV
HHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEE
ISNSDELDIINEYTPDLVIISSTLENEDPLRISVILRGKEEIRGVVIILQIDEDGMPLVV
EECCCCEEEHHCCCCCEEEEEECCCCCCCEEEEEEEECHHHCCEEEEEEEECCCCCCEEE
KGIELGVNDYFVYPIEESELLARIRTQLRRKQYQDNLHNDLEQSVNLAAKDGLTGLFNRR
EEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHCCE
YFDIHLKQMIEKANNESIKLYLLMCDIDNFKHVNDTYGHQAGDKVLTIVSRILKNTLRVT
EEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DLIARFGGEEFTILLTDIDISKAIETAERIRVKIEYMDFHIEDQIEPLQKTISIGVTEYK
HHHHHHCCCEEEEEEEECCHHHHHHHHHHEEEEEEEEEEEEHHHHHHHHHHHHCCCHHHH
KAESIESFIERADKAMYEAKTTGRNKVVKL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA