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The map label for this gene is btuB [H]

Identifier: 157373002

GI number: 157373002

Start: 5283340

End: 5285211

Strand: Reverse

Name: btuB [H]

Synonym: Spro_4770

Alternate gene names: 157373002

Gene position: 5285211-5283340 (Counterclockwise)

Preceding gene: 157373004

Following gene: 157373001

Centisome position: 97.0

GC content: 52.72

Gene sequence:

>1872_bases
ATGACAATTACAAAAAATACACTGCTGGCGGTGGTTTCTTCCATCACGGCTTTTTCTGGATGGGCGCAAGACAACACCAC
AGCCAATAACAGCGATGACCTGGTGGTTTCCGCCAACCGTTTTCCGCAGCCGGTTTCTTCCGTGCTGGCACCCACCTCTG
TCGTTACCCGTGACGAAATCGACCGCTGGCAGGCAAAAAGCCTGACCGATGTGATGCGCCGCCTGCCGGGGGTGGATGTT
GGGCAGAATGGTGGCCTGGGACAAAAAAGCTCGCTGTTTATCCGCGGTACCAACTCCAGCCATGTCCTGGTGCTGATCGA
CGGTATTCGCCTTAACCAGGCCGGCATCAGTGGTTCCTCCGACCTCAGCCAGATCCCGATTTCGCTGGTGCAGAAAGTCG
AATATATTCGCGGACCGCGTTCGGCTGTCTATGGTTCCGATGCCATCGGTGGCGTGGTGAACATTATTACCACCCGTGAA
AAGAACGGCACTACGCTTTCTGCCGGTATGGGTTCAAACGGCTATCAGGCCTATGACGCTTCCACTCAGCAACAGTTGGG
CGACAGCACGGTGGCGACGCTCGCCGGCAACTATACCTATACCAAAGGCTATGATGTAGTGGCTTACGGCAGTACCGGTA
TGCAGAGCCAGCAGGATCGCGATGGCTACATGAGTAAAGCGCTGTACGGCGCTGTTGACCATCAGTTCAATGAGGCCATT
AGCGGCTTTGTGCGTGGTTATGGTTATGACAACCGTACGGCTTACGATGCTTATTATTCCTCCCCGACCAGCCCACTGGT
AGATACCCGCAAGCTGTACAGTCAGACCTGGGATACCGGCCTGCGTTATAAAGAAGGTATCTACGCGACACAACTGATTG
GCAGTTATAGCCATAGCAAGGATTATGATTACGATCCTCGACTGGGTATGCATGACAGTTCGGCAAGTTTGGTGGATTCA
GAGCAATACAACCTGCAGTGGGGAAATACCCTGCAGTTGGGCCAGGGGACGGTCAGCTCAGGGGTTGATTGGCAACACCA
GGAGATCAAGCCGAACTCCACTACGGTCAACGGTGAGAAAAGTCAGCGTAACGCCGGTATCTATCTGACCGCTCAGCAGT
TGGTTGGCCCGGTAACCCTGGAAGGGGCGGTGCGTGGTGACGATCATTCTGAATTCGGCTGGCATGGTACCTGGCAAACC
AGCGCGGGCTGGGAGTTTGTCGAAGGGTACCGCTTTATTGCTTCCTATGGTACGGCGTTTAAAGCCCCAAATATGGGCCA
GCTGTATGGCAACTTTGGTAATAATACCGATTTGAAGCCGGAAGAAAGTAAACAGTGGGAAGGCGGTTTTGAAGGTTTGA
CAGGTCCAGTGACCTGGCGCGTTTCCGGCTACCGTAACGATATTGATAACCTGATTGATTCCACTGGGGAGACCAATTAC
GTTTATTACAATATCGGCAAGGCTACTATTAAAGGTATAGAAGCCACGGCTTCGTTTGATACCGGGCCGTTAACCCACCA
TATCGGCTACGACTATGTCGACCCACGCAATGCCAAAACCAACGAAGTGCTGCTGCGTCGCGCCAAACAGCAGGTGAAGT
ATGAGCTGGACTGGCAGGTCTATGATTTTGACTGGGCGGTCACCTATCAATACCTGGGGGAGCGCTATGACAAAGACTAC
AGCACCTACCCCGAGCAAAGCGTCAAACTCGGCGGTGTCAGCCTGTGGGATCTCGCAGTTTCGTATCCGGTCACATCTCA
TCTGACAGTTCGTGGTAGAATCGCCAACCTGTTTGATAAAGATTATGAGACGGCTTATGGCTACGCTACTCCAGGAAGAG
AATACTACCTCACTGGAAGCTATACCTTCTAA

Upstream 100 bases:

>100_bases
GCCCGAAGACCTGCCGGTGTTACGTCGCAATGTCCGTGGCCGTCGCGAAATACCGGCCATGATCCTGCGGCATCCGCCAA
TTAAGTTTGGATGCTTTTTC

Downstream 100 bases:

>100_bases
CACCACCGCGAAGACCCGTCCGACGGTACTGGTATTTGATTCCGGCGTCGGCGGGTTATCGGTGTATCAAGAGGTTCGGC
AACTGTTGCCGGACCTGCAC

Product: vitamin B12/cobalamin outer membrane transporter

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MTITKNTLLAVVSSITAFSGWAQDNTTANNSDDLVVSANRFPQPVSSVLAPTSVVTRDEIDRWQAKSLTDVMRRLPGVDV
GQNGGLGQKSSLFIRGTNSSHVLVLIDGIRLNQAGISGSSDLSQIPISLVQKVEYIRGPRSAVYGSDAIGGVVNIITTRE
KNGTTLSAGMGSNGYQAYDASTQQQLGDSTVATLAGNYTYTKGYDVVAYGSTGMQSQQDRDGYMSKALYGAVDHQFNEAI
SGFVRGYGYDNRTAYDAYYSSPTSPLVDTRKLYSQTWDTGLRYKEGIYATQLIGSYSHSKDYDYDPRLGMHDSSASLVDS
EQYNLQWGNTLQLGQGTVSSGVDWQHQEIKPNSTTVNGEKSQRNAGIYLTAQQLVGPVTLEGAVRGDDHSEFGWHGTWQT
SAGWEFVEGYRFIASYGTAFKAPNMGQLYGNFGNNTDLKPEESKQWEGGFEGLTGPVTWRVSGYRNDIDNLIDSTGETNY
VYYNIGKATIKGIEATASFDTGPLTHHIGYDYVDPRNAKTNEVLLRRAKQQVKYELDWQVYDFDWAVTYQYLGERYDKDY
STYPEQSVKLGGVSLWDLAVSYPVTSHLTVRGRIANLFDKDYETAYGYATPGREYYLTGSYTF

Sequences:

>Translated_623_residues
MTITKNTLLAVVSSITAFSGWAQDNTTANNSDDLVVSANRFPQPVSSVLAPTSVVTRDEIDRWQAKSLTDVMRRLPGVDV
GQNGGLGQKSSLFIRGTNSSHVLVLIDGIRLNQAGISGSSDLSQIPISLVQKVEYIRGPRSAVYGSDAIGGVVNIITTRE
KNGTTLSAGMGSNGYQAYDASTQQQLGDSTVATLAGNYTYTKGYDVVAYGSTGMQSQQDRDGYMSKALYGAVDHQFNEAI
SGFVRGYGYDNRTAYDAYYSSPTSPLVDTRKLYSQTWDTGLRYKEGIYATQLIGSYSHSKDYDYDPRLGMHDSSASLVDS
EQYNLQWGNTLQLGQGTVSSGVDWQHQEIKPNSTTVNGEKSQRNAGIYLTAQQLVGPVTLEGAVRGDDHSEFGWHGTWQT
SAGWEFVEGYRFIASYGTAFKAPNMGQLYGNFGNNTDLKPEESKQWEGGFEGLTGPVTWRVSGYRNDIDNLIDSTGETNY
VYYNIGKATIKGIEATASFDTGPLTHHIGYDYVDPRNAKTNEVLLRRAKQQVKYELDWQVYDFDWAVTYQYLGERYDKDY
STYPEQSVKLGGVSLWDLAVSYPVTSHLTVRGRIANLFDKDYETAYGYATPGREYYLTGSYTF
>Mature_622_residues
TITKNTLLAVVSSITAFSGWAQDNTTANNSDDLVVSANRFPQPVSSVLAPTSVVTRDEIDRWQAKSLTDVMRRLPGVDVG
QNGGLGQKSSLFIRGTNSSHVLVLIDGIRLNQAGISGSSDLSQIPISLVQKVEYIRGPRSAVYGSDAIGGVVNIITTREK
NGTTLSAGMGSNGYQAYDASTQQQLGDSTVATLAGNYTYTKGYDVVAYGSTGMQSQQDRDGYMSKALYGAVDHQFNEAIS
GFVRGYGYDNRTAYDAYYSSPTSPLVDTRKLYSQTWDTGLRYKEGIYATQLIGSYSHSKDYDYDPRLGMHDSSASLVDSE
QYNLQWGNTLQLGQGTVSSGVDWQHQEIKPNSTTVNGEKSQRNAGIYLTAQQLVGPVTLEGAVRGDDHSEFGWHGTWQTS
AGWEFVEGYRFIASYGTAFKAPNMGQLYGNFGNNTDLKPEESKQWEGGFEGLTGPVTWRVSGYRNDIDNLIDSTGETNYV
YYNIGKATIKGIEATASFDTGPLTHHIGYDYVDPRNAKTNEVLLRRAKQQVKYELDWQVYDFDWAVTYQYLGERYDKDYS
TYPEQSVKLGGVSLWDLAVSYPVTSHLTVRGRIANLFDKDYETAYGYATPGREYYLTGSYTF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=621, Percent_Identity=69.4041867954911, Blast_Score=874, Evalue=0.0,
Organism=Escherichia coli, GI1788478, Length=685, Percent_Identity=25.8394160583942, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1786798, Length=740, Percent_Identity=23.3783783783784, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787024, Length=201, Percent_Identity=28.3582089552239, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1787723, Length=684, Percent_Identity=24.5614035087719, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68790; Mature: 68659

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITKNTLLAVVSSITAFSGWAQDNTTANNSDDLVVSANRFPQPVSSVLAPTSVVTRDEI
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHCCCHHHHHHHH
DRWQAKSLTDVMRRLPGVDVGQNGGLGQKSSLFIRGTNSSHVLVLIDGIRLNQAGISGSS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECEEEECCCCCCCC
DLSQIPISLVQKVEYIRGPRSAVYGSDAIGGVVNIITTREKNGTTLSAGMGSNGYQAYDA
CHHHHHHHHHHHHHHHCCCCHHCCCCCHHCCEEEEEEECCCCCCEEEECCCCCCCEECCC
STQQQLGDSTVATLAGNYTYTKGYDVVAYGSTGMQSQQDRDGYMSKALYGAVDHQFNEAI
CHHHHCCCCEEEEEECCEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SGFVRGYGYDNRTAYDAYYSSPTSPLVDTRKLYSQTWDTGLRYKEGIYATQLIGSYSHSK
HHHHHHCCCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHCCCCHHCCCHHHHHHHCCCCCC
DYDYDPRLGMHDSSASLVDSEQYNLQWGNTLQLGQGTVSSGVDWQHQEIKPNSTTVNGEK
CCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCHHCCCCCCCEECCCC
SQRNAGIYLTAQQLVGPVTLEGAVRGDDHSEFGWHGTWQTSAGWEFVEGYRFIASYGTAF
CCCCCCEEEEHHHHCCCEEEECEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCC
KAPNMGQLYGNFGNNTDLKPEESKQWEGGFEGLTGPVTWRVSGYRNDIDNLIDSTGETNY
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCE
VYYNIGKATIKGIEATASFDTGPLTHHIGYDYVDPRNAKTNEVLLRRAKQQVKYELDWQV
EEEECCCCEEECEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEECEEE
YDFDWAVTYQYLGERYDKDYSTYPEQSVKLGGVSLWDLAVSYPVTSHLTVRGRIANLFDK
EEEEHEEHHHHHHHHHCCCCCCCCCCCEEECCEEEEHHEECCCCCCEEEEEHHHHHHHCC
DYETAYGYATPGREYYLTGSYTF
CCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
TITKNTLLAVVSSITAFSGWAQDNTTANNSDDLVVSANRFPQPVSSVLAPTSVVTRDEI
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHCCCHHHHHHHH
DRWQAKSLTDVMRRLPGVDVGQNGGLGQKSSLFIRGTNSSHVLVLIDGIRLNQAGISGSS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECEEEECCCCCCCC
DLSQIPISLVQKVEYIRGPRSAVYGSDAIGGVVNIITTREKNGTTLSAGMGSNGYQAYDA
CHHHHHHHHHHHHHHHCCCCHHCCCCCHHCCEEEEEEECCCCCCEEEECCCCCCCEECCC
STQQQLGDSTVATLAGNYTYTKGYDVVAYGSTGMQSQQDRDGYMSKALYGAVDHQFNEAI
CHHHHCCCCEEEEEECCEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SGFVRGYGYDNRTAYDAYYSSPTSPLVDTRKLYSQTWDTGLRYKEGIYATQLIGSYSHSK
HHHHHHCCCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHCCCCHHCCCHHHHHHHCCCCCC
DYDYDPRLGMHDSSASLVDSEQYNLQWGNTLQLGQGTVSSGVDWQHQEIKPNSTTVNGEK
CCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCHHCCCCCCCEECCCC
SQRNAGIYLTAQQLVGPVTLEGAVRGDDHSEFGWHGTWQTSAGWEFVEGYRFIASYGTAF
CCCCCCEEEEHHHHCCCEEEECEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCC
KAPNMGQLYGNFGNNTDLKPEESKQWEGGFEGLTGPVTWRVSGYRNDIDNLIDSTGETNY
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCE
VYYNIGKATIKGIEATASFDTGPLTHHIGYDYVDPRNAKTNEVLLRRAKQQVKYELDWQV
EEEECCCCEEECEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEECEEE
YDFDWAVTYQYLGERYDKDYSTYPEQSVKLGGVSLWDLAVSYPVTSHLTVRGRIANLFDK
EEEEHEEHHHHHHHHHCCCCCCCCCCCEEECCEEEEHHEECCCCCCEEEEEHHHHHHHCC
DYETAYGYATPGREYYLTGSYTF
CCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA