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The map label for this gene is gltX

Identifier: 157371654

GI number: 157371654

Start: 3779962

End: 3781371

Strand: Reverse

Name: gltX

Synonym: Spro_3417

Alternate gene names: 157371654

Gene position: 3781371-3779962 (Counterclockwise)

Preceding gene: 157371655

Following gene: 157371652

Centisome position: 69.4

GC content: 56.38

Gene sequence:

>1410_bases
ATGAAAATCAAAACCCGTTTTGCACCGAGCCCAACCGGCTATCTTCACGTCGGTGGCGCCCGTACTGCGCTTTACTCCTG
GTTGTTCAGCCGCCATGCGGGCGGTGAGTTCGTTCTGCGTATCGAAGATACCGATCTGGAACGCTCAACCCAGGACGCGA
TCGACGCAATTATGGATGGCATGAACTGGTTAAACCTGGATTGGAATGAAGGCCCGTACTTCCAGACCAAGCGTTTTGAC
CGCTATAACGCGGTGATTGACGACATGCTGGTGCAGGATACCGCCTATAAATGCTATTGCTCGAAGGAACGTCTGGAAGC
GCTGCGTGAAAAGCAGATGGAAAACGGCGAAAAACCGCGTTACGACGGCCACTGCCGCGACAGCCAGTGCAGCCATACCG
ATGACGAGCCACACGTGGTGCGTTTCCGTAACCCGCAGGAAGGCTCGGTGATCTTCGACGACAAGATCCGCGGCCCGATC
GAATTTAGCAACCAGGAGCTGGATGACCTGATCATCCGCCGCACCGACGGTTCACCAACCTATAACTTCTGCGTAGTGGT
AGATGACTGGGATATGGAAATCAGCCACGTGATCCGCGGCGAAGACCACATCAACAACACCCCGCGCCAGATCAACATCC
TGAAAGCGCTGGGTGCGCCGGTGCCGGAATACGCGCACGTTTCGATGATCCTCGGTGACGACGGTAAAAAACTGTCGAAA
CGCCACGGTGCGGTAGGGGTGATGCAGTACCGTGACGACGGCTACCTGCCGCAGGCGCTGCTGAACTATCTGGTGCGTCT
GGGCTGGTCTCATGGCGACCAGGAAATCTTCTCTATTGATGAGATGAAAGAGTTCTTTACCCTGGAAGCGATCAACAAAT
CCGCCAGTGCCTTCAATACCGAGAAGTTGCAGTGGCTGAACCACCATTACATTAACCATATGCCGGCCGAAGAAGTGGCA
GTGCACCTGGCGTGGCATGTTGAGCAACTGGGTATCGAGACCCGCAATGGCCCTGAGCTGAAAGATATCGTTAAGCTGCT
GGGCGAGCGCTGCAAAACGCTCAAGGAAATGGCCGAGTCTTGCCGTTACTTCTACGAAGACTTCAGCGAGTTCGACGCTG
ACGCTGCCAAGAAACACCTGCGTCCGGTCGCTCGTCAGCCACTGGAAGCGGTGCGCGCCAAGCTGGCCGCTATCACTGCC
TGGACGCCGGAAAACGTGCATGACGCCATTCAGGGCACGGCAGATGAGCTGGGCGTAGGTATGGGCAAGGTAGGTATGCC
GCTGCGCGTGGCGGTTACCGGTGCCGGTCAGTCACCGGGTATGGATGTCACCGTTCATGCCATCGGTCAGAACCGTTCTC
TGCAGCGTATCGACCTGGCGTTGGCTTATATTGCCGGGCGTGAAGCTTAA

Upstream 100 bases:

>100_bases
AGGAATTAGCGCGTGGTGGTACAATGTTGCCCACTTTTTTAAATTCCGAGCAATCGTCGGCAACCGCCGACACGCGTTGC
GTAATAAGGCAGTGAAACCA

Downstream 100 bases:

>100_bases
GGCTGAGCGTTTCCCTGCCAGTTAATGACAAAGCCCCGTGGAGCAATCCCGGGGCTTTTTTGTATCTGCTATTTGATGGC
GAATTTCTGGTGCGCCGCGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHAGGEFVLRIEDTDLERSTQDAIDAIMDGMNWLNLDWNEGPYFQTKRFD
RYNAVIDDMLVQDTAYKCYCSKERLEALREKQMENGEKPRYDGHCRDSQCSHTDDEPHVVRFRNPQEGSVIFDDKIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEISHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGYLPQALLNYLVRLGWSHGDQEIFSIDEMKEFFTLEAINKSASAFNTEKLQWLNHHYINHMPAEEVA
VHLAWHVEQLGIETRNGPELKDIVKLLGERCKTLKEMAESCRYFYEDFSEFDADAAKKHLRPVARQPLEAVRAKLAAITA
WTPENVHDAIQGTADELGVGMGKVGMPLRVAVTGAGQSPGMDVTVHAIGQNRSLQRIDLALAYIAGREA

Sequences:

>Translated_469_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHAGGEFVLRIEDTDLERSTQDAIDAIMDGMNWLNLDWNEGPYFQTKRFD
RYNAVIDDMLVQDTAYKCYCSKERLEALREKQMENGEKPRYDGHCRDSQCSHTDDEPHVVRFRNPQEGSVIFDDKIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEISHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGYLPQALLNYLVRLGWSHGDQEIFSIDEMKEFFTLEAINKSASAFNTEKLQWLNHHYINHMPAEEVA
VHLAWHVEQLGIETRNGPELKDIVKLLGERCKTLKEMAESCRYFYEDFSEFDADAAKKHLRPVARQPLEAVRAKLAAITA
WTPENVHDAIQGTADELGVGMGKVGMPLRVAVTGAGQSPGMDVTVHAIGQNRSLQRIDLALAYIAGREA
>Mature_469_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHAGGEFVLRIEDTDLERSTQDAIDAIMDGMNWLNLDWNEGPYFQTKRFD
RYNAVIDDMLVQDTAYKCYCSKERLEALREKQMENGEKPRYDGHCRDSQCSHTDDEPHVVRFRNPQEGSVIFDDKIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEISHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGYLPQALLNYLVRLGWSHGDQEIFSIDEMKEFFTLEAINKSASAFNTEKLQWLNHHYINHMPAEEVA
VHLAWHVEQLGIETRNGPELKDIVKLLGERCKTLKEMAESCRYFYEDFSEFDADAAKKHLRPVARQPLEAVRAKLAAITA
WTPENVHDAIQGTADELGVGMGKVGMPLRVAVTGAGQSPGMDVTVHAIGQNRSLQRIDLALAYIAGREA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=493, Percent_Identity=30.6288032454361, Blast_Score=213, Evalue=4e-55,
Organism=Escherichia coli, GI1788743, Length=468, Percent_Identity=83.5470085470085, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=241, Percent_Identity=35.2697095435685, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=474, Percent_Identity=30.8016877637131, Blast_Score=216, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6324540, Length=493, Percent_Identity=30.0202839756592, Blast_Score=236, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=230, Percent_Identity=24.7826086956522, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=213, Percent_Identity=23.4741784037559, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24665321, Length=490, Percent_Identity=30.6122448979592, Blast_Score=248, Evalue=8e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_SERP5 (A8GHC5)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479643.1
- ProteinModelPortal:   A8GHC5
- SMR:   A8GHC5
- STRING:   A8GHC5
- GeneID:   5603084
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3417
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   SPRO399741:SPRO_3417-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53181; Mature: 53181

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHAGGEFVLRIEDTDLERSTQDAIDAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCC
MNWLNLDWNEGPYFQTKRFDRYNAVIDDMLVQDTAYKCYCSKERLEALREKQMENGEKPR
CCEEECCCCCCCCCCCHHCHHHHHHHHHHHHHCCHHHEEECHHHHHHHHHHHHHCCCCCC
YDGHCRDSQCSHTDDEPHVVRFRNPQEGSVIFDDKIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVVDDWDMEISHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
EEEEEEEECCCCHHHHHHCCCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHH
RHGAVGVMQYRDDGYLPQALLNYLVRLGWSHGDQEIFSIDEMKEFFTLEAINKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCH
EKLQWLNHHYINHMPAEEVAVHLAWHVEQLGIETRNGPELKDIVKLLGERCKTLKEMAES
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRYFYEDFSEFDADAAKKHLRPVARQPLEAVRAKLAAITAWTPENVHDAIQGTADELGVG
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCCHHHHCCC
MGKVGMPLRVAVTGAGQSPGMDVTVHAIGQNRSLQRIDLALAYIAGREA
CCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHAGGEFVLRIEDTDLERSTQDAIDAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCC
MNWLNLDWNEGPYFQTKRFDRYNAVIDDMLVQDTAYKCYCSKERLEALREKQMENGEKPR
CCEEECCCCCCCCCCCHHCHHHHHHHHHHHHHCCHHHEEECHHHHHHHHHHHHHCCCCCC
YDGHCRDSQCSHTDDEPHVVRFRNPQEGSVIFDDKIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVVDDWDMEISHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSK
EEEEEEEECCCCHHHHHHCCCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHH
RHGAVGVMQYRDDGYLPQALLNYLVRLGWSHGDQEIFSIDEMKEFFTLEAINKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCH
EKLQWLNHHYINHMPAEEVAVHLAWHVEQLGIETRNGPELKDIVKLLGERCKTLKEMAES
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRYFYEDFSEFDADAAKKHLRPVARQPLEAVRAKLAAITAWTPENVHDAIQGTADELGVG
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCCHHHHCCC
MGKVGMPLRVAVTGAGQSPGMDVTVHAIGQNRSLQRIDLALAYIAGREA
CCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA