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The map label for this gene is pdxB

Identifier: 157371573

GI number: 157371573

Start: 3691375

End: 3692496

Strand: Reverse

Name: pdxB

Synonym: Spro_3336

Alternate gene names: 157371573

Gene position: 3692496-3691375 (Counterclockwise)

Preceding gene: 157371574

Following gene: 157371572

Centisome position: 67.77

GC content: 59.0

Gene sequence:

>1122_bases
GTGAAAATCCTGGTTGATGAAAATATGCCGTACGCGACTGAGTTGTTCAGTCGTTTGGGGAATGTGCAGGCGGTGCCTGG
CCGTCCGATCCCGAGTGATGCGCTGGCCGATGCCGATGCGCTGATGGTGCGTTCGGTGACTAAAGTGAATGAAGCCTTGC
TGGCCGGTACCCGCATTGGTTTTGTCGGCACCGCGACCGCCGGTACCGACCACGTAGACGACGTTTGGCTGCAACAGCAG
GGCATCGGTTTTTCTGCCGCACCGGGCTGCAACGCCATTGCGGTAGTGGAATATGTGTTCTCCGCGCTGATGCTGCTGGC
CGAACGCGATGGTTTCCAACTGCGGGATAAAACCGTCGGTATTATCGGTGTTGGCAATGTAGGCTCACGCCTGGATGCCC
GCCTGAAAGCCTTGGGCGTCCGTACCTTGTTATGCGATCCGCCGCGGGCTGAACGCGGTGATGCCGGTGAGTTTTGGCCG
TTGGAAAAGCTGGTGGCGGAAGCCGATGTGCTCACTTTCCATACGCCATTGAATAAATCCGGCCCTTACAACTCGCTGCA
TCTGGTGGACGCGGAACTGCTGGCGGCGTTGCCGGACAATCGCATTCTGATCAACGCCTGTCGCGGTGCGGTGGTGGACA
ATGCTGCGCTGTTGCAGGCACTGGAGAAAGGCAAAAAACTGAGTACCGTGCTGGACGTGTGGGAGCCGGAACCGGAGCTT
TCGCTGCCGTTGCTGGCGCGGGTGGATATTGGCACCGCGCATATTGCCGGTTACACGCTGGAAGGCAAGGCACGCGGCAC
CACTCAGGTGTTTGAGGCCTTCAGCCGCCACCTGGGCCAGCCGCAACAGATAGAGCTGGCCTCGCTGCTGCCGGTGCCCG
AATTCAGCCAGATCCAACTCAATGGGCCGCTGGATGAAGGCAAATTAAAACGATTGATGCACTTGGTGTATGATGTGCGC
CGCGATGATGCGCCGCTGCGCAAAGTGGCCGGGCAACCGGGCGAGTTTGATCGTCTGCGCAAGCATTACCAGGAACGCCG
CGAATGGTCTTCATTACGCGTCCAGTGCGATGACAGCGCCAGTGCGGAGCTGTTGCACAAGCTGGGTTTCCTGACGGCAT
AA

Upstream 100 bases:

>100_bases
TTCAGCAAATCGCCGGTACCACCCCCGAGCGCTTTCGTCGGGCATGATTCAGTGGCACACTATTTCCCAGCGAAATAGCC
GTAAACCGTCGGAGCAATAA

Downstream 100 bases:

>100_bases
GGCTTTCTTAAGCCACGGCGACTAATTTTGTTATCAGGGCGGTGCAGTAGCCGCCCATCTGCATTTGAATAAATACTCTG
GAGAAACCCAATGTCTGACG

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKILVDENMPYATELFSRLGNVQAVPGRPIPSDALADADALMVRSVTKVNEALLAGTRIGFVGTATAGTDHVDDVWLQQQ
GIGFSAAPGCNAIAVVEYVFSALMLLAERDGFQLRDKTVGIIGVGNVGSRLDARLKALGVRTLLCDPPRAERGDAGEFWP
LEKLVAEADVLTFHTPLNKSGPYNSLHLVDAELLAALPDNRILINACRGAVVDNAALLQALEKGKKLSTVLDVWEPEPEL
SLPLLARVDIGTAHIAGYTLEGKARGTTQVFEAFSRHLGQPQQIELASLLPVPEFSQIQLNGPLDEGKLKRLMHLVYDVR
RDDAPLRKVAGQPGEFDRLRKHYQERREWSSLRVQCDDSASAELLHKLGFLTA

Sequences:

>Translated_373_residues
MKILVDENMPYATELFSRLGNVQAVPGRPIPSDALADADALMVRSVTKVNEALLAGTRIGFVGTATAGTDHVDDVWLQQQ
GIGFSAAPGCNAIAVVEYVFSALMLLAERDGFQLRDKTVGIIGVGNVGSRLDARLKALGVRTLLCDPPRAERGDAGEFWP
LEKLVAEADVLTFHTPLNKSGPYNSLHLVDAELLAALPDNRILINACRGAVVDNAALLQALEKGKKLSTVLDVWEPEPEL
SLPLLARVDIGTAHIAGYTLEGKARGTTQVFEAFSRHLGQPQQIELASLLPVPEFSQIQLNGPLDEGKLKRLMHLVYDVR
RDDAPLRKVAGQPGEFDRLRKHYQERREWSSLRVQCDDSASAELLHKLGFLTA
>Mature_373_residues
MKILVDENMPYATELFSRLGNVQAVPGRPIPSDALADADALMVRSVTKVNEALLAGTRIGFVGTATAGTDHVDDVWLQQQ
GIGFSAAPGCNAIAVVEYVFSALMLLAERDGFQLRDKTVGIIGVGNVGSRLDARLKALGVRTLLCDPPRAERGDAGEFWP
LEKLVAEADVLTFHTPLNKSGPYNSLHLVDAELLAALPDNRILINACRGAVVDNAALLQALEKGKKLSTVLDVWEPEPEL
SLPLLARVDIGTAHIAGYTLEGKARGTTQVFEAFSRHLGQPQQIELASLLPVPEFSQIQLNGPLDEGKLKRLMHLVYDVR
RDDAPLRKVAGQPGEFDRLRKHYQERREWSSLRVQCDDSASAELLHKLGFLTA

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=300, Percent_Identity=27.3333333333333, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI6912396, Length=241, Percent_Identity=27.3858921161826, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI145580578, Length=130, Percent_Identity=32.3076923076923, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI4557499, Length=130, Percent_Identity=32.3076923076923, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI61743967, Length=130, Percent_Identity=33.0769230769231, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI4557497, Length=130, Percent_Identity=33.0769230769231, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI145580575, Length=130, Percent_Identity=32.3076923076923, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788660, Length=370, Percent_Identity=74.054054054054, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI1787645, Length=202, Percent_Identity=29.2079207920792, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789279, Length=309, Percent_Identity=27.831715210356, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082289, Length=160, Percent_Identity=33.125, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=263, Percent_Identity=26.615969581749, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322116, Length=362, Percent_Identity=27.0718232044199, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=327, Percent_Identity=27.217125382263, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=168, Percent_Identity=32.1428571428571, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324964, Length=271, Percent_Identity=26.1992619926199, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19921140, Length=269, Percent_Identity=30.8550185873606, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574286, Length=232, Percent_Identity=27.1551724137931, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24646446, Length=228, Percent_Identity=23.6842105263158, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646448, Length=228, Percent_Identity=23.6842105263158, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646452, Length=228, Percent_Identity=23.6842105263158, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646450, Length=228, Percent_Identity=23.6842105263158, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI62472511, Length=228, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_SERP5 (A8GH44)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479562.1
- ProteinModelPortal:   A8GH44
- SMR:   A8GH44
- STRING:   A8GH44
- GeneID:   5602543
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3336
- eggNOG:   COG0111
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- ProtClustDB:   CLSK2393827
- BioCyc:   SPRO399741:SPRO_3336-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 40634; Mature: 40634

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 208-208 ACT_SITE 237-237 ACT_SITE 254-254 BINDING 45-45 BINDING 66-66 BINDING 146-146 BINDING 175-175 BINDING 232-232 BINDING 257-257 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVDENMPYATELFSRLGNVQAVPGRPIPSDALADADALMVRSVTKVNEALLAGTRIG
CEEEECCCCCHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FVGTATAGTDHVDDVWLQQQGIGFSAAPGCNAIAVVEYVFSALMLLAERDGFQLRDKTVG
EEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCEEE
IIGVGNVGSRLDARLKALGVRTLLCDPPRAERGDAGEFWPLEKLVAEADVLTFHTPLNKS
EEEECCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCC
GPYNSLHLVDAELLAALPDNRILINACRGAVVDNAALLQALEKGKKLSTVLDVWEPEPEL
CCCCCEEEEHHHHHHHCCCCCEEEEECCCCHHCHHHHHHHHHHCCHHHHHHHCCCCCCCC
SLPLLARVDIGTAHIAGYTLEGKARGTTQVFEAFSRHLGQPQQIELASLLPVPEFSQIQL
CCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEHHHHCCCCCCCEEEE
NGPLDEGKLKRLMHLVYDVRRDDAPLRKVAGQPGEFDRLRKHYQERREWSSLRVQCDDSA
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEECCCH
SAELLHKLGFLTA
HHHHHHHHHCCCC
>Mature Secondary Structure
MKILVDENMPYATELFSRLGNVQAVPGRPIPSDALADADALMVRSVTKVNEALLAGTRIG
CEEEECCCCCHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FVGTATAGTDHVDDVWLQQQGIGFSAAPGCNAIAVVEYVFSALMLLAERDGFQLRDKTVG
EEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCEEE
IIGVGNVGSRLDARLKALGVRTLLCDPPRAERGDAGEFWPLEKLVAEADVLTFHTPLNKS
EEEECCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCC
GPYNSLHLVDAELLAALPDNRILINACRGAVVDNAALLQALEKGKKLSTVLDVWEPEPEL
CCCCCEEEEHHHHHHHCCCCCEEEEECCCCHHCHHHHHHHHHHCCHHHHHHHCCCCCCCC
SLPLLARVDIGTAHIAGYTLEGKARGTTQVFEAFSRHLGQPQQIELASLLPVPEFSQIQL
CCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEHHHHCCCCCCCEEEE
NGPLDEGKLKRLMHLVYDVRRDDAPLRKVAGQPGEFDRLRKHYQERREWSSLRVQCDDSA
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEECCCH
SAELLHKLGFLTA
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA