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The map label for this gene is truA [H]

Identifier: 157371571

GI number: 157371571

Start: 3689453

End: 3690274

Strand: Reverse

Name: truA [H]

Synonym: Spro_3334

Alternate gene names: 157371571

Gene position: 3690274-3689453 (Counterclockwise)

Preceding gene: 157371572

Following gene: 157371570

Centisome position: 67.73

GC content: 56.81

Gene sequence:

>822_bases
ATGTCTGAAGTTGTCTTGCCCACCCAGCCGACGTTGAAAATCGCGCTGGGGATTGAATATGACGGCAGCCAGTATTACGG
CTGGCAGCGTCAGCAGGAAGTCGCCAGCGTGCAGGCCTGTCTGGAACAGGCGTTGAGCAAGGTAGCGGATGCGCCGATCA
ACGTGTTGTGCGCCGGACGTACCGACGCCGGAGTGCATGCCACCGGTCAGGTGGTGCATTTCGAAACCACCGCGCAACGC
AAGGACGCGGCCTGGACGATGGGTGTCAACACCCATCTGCCGCCGGATATCGCCGTGCGTTGGGTGACTGGCGTGAATGA
AGATTTTCATGCCCGTTTCAGCGCGACTGCGCGTCGGTATCGCTACATTATTTATAACCATCGCTATCGCCCGGCGGTAT
TGCAGAAGGGAATGACTCACTTTTATCACCCGCTGGATGCCGAACGGATGGAAAGGGCGGCGCAGGCGCTGCTGGGCGAG
AACGACTTTACTTCGTTCCGCACCGTGCAATGTCAGTCACGCACGCCGTGGCGTAACGTAAAACACGTTAAGGTTACGCG
CTATGGCGAATATATTGTGGTAGATATCAAGGCGAATGCCTTTGTGCATCATATGGTTCGCAACATTGTCGGTAGCCTGA
TGGAAATTGGCTGCGGCAATCAGGATGAGAACTGGATGGCCGAATTACTGGCGCTGAAGGATCGTAATCTGGCTGCGGCG
ACGGCGCGGGCCGACGGGTTATACCTGGTTAGCGTTGACTACCCTGAGCACTTTGCCATACCCCGGCCGGCAATGGGGCC
ACTGTTTTTACCCGACGACTAA

Upstream 100 bases:

>100_bases
AAATCCTGCAGTTCTGGTCGGTGGCCGACAACGTGCGTTTCGGCGGCGCGCTGATGGCGATCGAAACCGCAGAACGTCTG
GTGCAGGAGCAGATGTACTG

Downstream 100 bases:

>100_bases
ATACTGGCGCGCCCGACGCGTTACTCCCTTGTGTAGCTGCCGGGTGGGTGAGACACCCGGTAAAAGAGAGAACCTATGGA
CATCATCAAGTTTGTAATTG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSEVVLPTQPTLKIALGIEYDGSQYYGWQRQQEVASVQACLEQALSKVADAPINVLCAGRTDAGVHATGQVVHFETTAQR
KDAAWTMGVNTHLPPDIAVRWVTGVNEDFHARFSATARRYRYIIYNHRYRPAVLQKGMTHFYHPLDAERMERAAQALLGE
NDFTSFRTVQCQSRTPWRNVKHVKVTRYGEYIVVDIKANAFVHHMVRNIVGSLMEIGCGNQDENWMAELLALKDRNLAAA
TARADGLYLVSVDYPEHFAIPRPAMGPLFLPDD

Sequences:

>Translated_273_residues
MSEVVLPTQPTLKIALGIEYDGSQYYGWQRQQEVASVQACLEQALSKVADAPINVLCAGRTDAGVHATGQVVHFETTAQR
KDAAWTMGVNTHLPPDIAVRWVTGVNEDFHARFSATARRYRYIIYNHRYRPAVLQKGMTHFYHPLDAERMERAAQALLGE
NDFTSFRTVQCQSRTPWRNVKHVKVTRYGEYIVVDIKANAFVHHMVRNIVGSLMEIGCGNQDENWMAELLALKDRNLAAA
TARADGLYLVSVDYPEHFAIPRPAMGPLFLPDD
>Mature_272_residues
SEVVLPTQPTLKIALGIEYDGSQYYGWQRQQEVASVQACLEQALSKVADAPINVLCAGRTDAGVHATGQVVHFETTAQRK
DAAWTMGVNTHLPPDIAVRWVTGVNEDFHARFSATARRYRYIIYNHRYRPAVLQKGMTHFYHPLDAERMERAAQALLGEN
DFTSFRTVQCQSRTPWRNVKHVKVTRYGEYIVVDIKANAFVHHMVRNIVGSLMEIGCGNQDENWMAELLALKDRNLAAAT
ARADGLYLVSVDYPEHFAIPRPAMGPLFLPDD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=284, Percent_Identity=28.169014084507, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788657, Length=263, Percent_Identity=79.0874524714829, Blast_Score=440, Evalue=1e-125,
Organism=Drosophila melanogaster, GI116007752, Length=299, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30772; Mature: 30641

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVVLPTQPTLKIALGIEYDGSQYYGWQRQQEVASVQACLEQALSKVADAPINVLCAGR
CCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
TDAGVHATGQVVHFETTAQRKDAAWTMGVNTHLPPDIAVRWVTGVNEDFHARFSATARRY
CCCCCEECCEEEEEECCCCCCCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHE
RYIIYNHRYRPAVLQKGMTHFYHPLDAERMERAAQALLGENDFTSFRTVQCQSRTPWRNV
EEEEEECCCCHHHHHCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
KHVKVTRYGEYIVVDIKANAFVHHMVRNIVGSLMEIGCGNQDENWMAELLALKDRNLAAA
EEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEE
TARADGLYLVSVDYPEHFAIPRPAMGPLFLPDD
EECCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEVVLPTQPTLKIALGIEYDGSQYYGWQRQQEVASVQACLEQALSKVADAPINVLCAGR
CCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
TDAGVHATGQVVHFETTAQRKDAAWTMGVNTHLPPDIAVRWVTGVNEDFHARFSATARRY
CCCCCEECCEEEEEECCCCCCCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHE
RYIIYNHRYRPAVLQKGMTHFYHPLDAERMERAAQALLGENDFTSFRTVQCQSRTPWRNV
EEEEEECCCCHHHHHCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
KHVKVTRYGEYIVVDIKANAFVHHMVRNIVGSLMEIGCGNQDENWMAELLALKDRNLAAA
EEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEE
TARADGLYLVSVDYPEHFAIPRPAMGPLFLPDD
EECCCCEEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA