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The map label for this gene is nuoB [H]

Identifier: 157371544

GI number: 157371544

Start: 3662366

End: 3663040

Strand: Reverse

Name: nuoB [H]

Synonym: Spro_3307

Alternate gene names: 157371544

Gene position: 3663040-3662366 (Counterclockwise)

Preceding gene: 157371545

Following gene: 157371543

Centisome position: 67.23

GC content: 55.85

Gene sequence:

>675_bases
ATGGACTATACGCTCACCCGCATAGACCCGAACGGTGAGAACGACCGTTACCCCCTGCAAAAACAGGAGGTCGTCGCCGA
TCCCCTGGATCAACAGGTTCACCGCACGGTTTACATGGGTAAACTCGAACATGCATTGCATGACATGGTGAACTGGGGGC
GTAAAAACTCTATTTGGCCGTATAACTTCGGTCTTTCGTGCTGCTACGTGGAGATGGTGACGTCGTTTACCGCCGTTCAC
GACGTGGCCCGCTTTGGTGCCGAAGTTCTGCGTGCATCGCCACGTCAGGCAGACCTGATGGTGGTGGCCGGTACCTGCTT
TACCAAGATGGCCCCGGTTATTCAGCGTCTGTACGAACAGATGCTGGAGCCAAAATGGGTGATCTCCATGGGTGCCTGCG
CCAACTCCGGCGGCATGTACGATATTTATTCCGTAGTACAGGGCGTGGACAAGTTCCTGCCGGTCGACGTGTACATTCCT
GGTTGCCCACCGCGCCCGGAAGCTTACATGCAGGCACTGATGCTGTTGCAGGAATCTATTGGCAAAGAACGCCGCCCTCT
GTCCTGGGTTGTCGGCGATCAGGGGGTTTACCGCGCCAACATGCAGTCTGAAAGAGAGCGTAAACACGGCGAGCGTATCG
CTGTGACCAACCTTCGGACGCCTGACGAGATTTAA

Upstream 100 bases:

>100_bases
ATTGGACTCCGGTGCGCTCCAGGCGCCAGAGCAAGCCAGGCACAATCAAAAACGCCACCAACAGTCACCCGCAGTAGTCG
GTTAAAAACGAGGCATTAAG

Downstream 100 bases:

>100_bases
GACGTTCATTTAACGTCCATCTTAGTGCGCTATTGGTTAACACAGCGAAAAGTGAACTTGCGGCGAAATAACCCTTCAGT
GTGGTGAAAAAAATTATGAC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MDYTLTRIDPNGENDRYPLQKQEVVADPLDQQVHRTVYMGKLEHALHDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVH
DVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIP
GCPPRPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKHGERIAVTNLRTPDEI

Sequences:

>Translated_224_residues
MDYTLTRIDPNGENDRYPLQKQEVVADPLDQQVHRTVYMGKLEHALHDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVH
DVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIP
GCPPRPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKHGERIAVTNLRTPDEI
>Mature_224_residues
MDYTLTRIDPNGENDRYPLQKQEVVADPLDQQVHRTVYMGKLEHALHDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVH
DVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIP
GCPPRPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKHGERIAVTNLRTPDEI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=145, Percent_Identity=53.7931034482759, Blast_Score=170, Evalue=1e-42,
Organism=Escherichia coli, GI1788624, Length=224, Percent_Identity=92.8571428571429, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=41.8032786885246, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=40.9836065573771, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=153, Percent_Identity=50.9803921568627, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI18859983, Length=145, Percent_Identity=53.1034482758621, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24642371, Length=145, Percent_Identity=53.1034482758621, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24651058, Length=145, Percent_Identity=53.1034482758621, Blast_Score=167, Evalue=7e-42,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 25520; Mature: 25520

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTLTRIDPNGENDRYPLQKQEVVADPLDQQVHRTVYMGKLEHALHDMVNWGRKNSIWP
CCCEEEEECCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHH
MLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIPGCPPRPEAYMQALMLLQESI
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
GKERRPLSWVVGDQGVYRANMQSERERKHGERIAVTNLRTPDEI
CCCCCCCEEEECCCCEEECCHHHHHHHHCCCEEEEECCCCCCCC
>Mature Secondary Structure
MDYTLTRIDPNGENDRYPLQKQEVVADPLDQQVHRTVYMGKLEHALHDMVNWGRKNSIWP
CCCEEEEECCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHH
MLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIPGCPPRPEAYMQALMLLQESI
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
GKERRPLSWVVGDQGVYRANMQSERERKHGERIAVTNLRTPDEI
CCCCCCCEEEECCCCEEECCHHHHHHHHCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA