Click here to switch to the map view.

The map label for this gene is nuoI [H]

Identifier: 157371538

GI number: 157371538

Start: 3654222

End: 3654764

Strand: Reverse

Name: nuoI [H]

Synonym: Spro_3301

Alternate gene names: 157371538

Gene position: 3654764-3654222 (Counterclockwise)

Preceding gene: 157371539

Following gene: 157371537

Centisome position: 67.07

GC content: 56.17

Gene sequence:

>543_bases
ATGACATTGAAAGAGTTATTGGTTGGTTTCGGCACCCAGGTGCGCAGCATTTGGATGATAGGCCTGCACGCCTTCGCCAA
GCGCGAAACCCAGATGTATCCGGAAGAACCGGTTTACCTGCCGCCGCGCTACCGTGGCCGCATCGTGCTGACGCGCGATC
CGGACGGCGAAGAGCGCTGCGTGGCTTGTAACCTGTGTGCGGTTGCCTGCCCGGTGGGCTGTATCTCGCTGCAAAAAGCA
GAACAGAAAGATGGCCGTTGGTACCCGGAGTTCTTCCGCATCAACTTCTCGCGCTGCATTTTCTGCGGCCTGTGCGAAGA
AGCTTGCCCAACGACCGCGATCCAGCTGACGCCGGATTTCGAAATGGGTGAGTTCAAGCGCCAGGATCTGGTGTACGAAA
AAGAAGATCTGTTGATCTCGGGGCCGGGTAAATATCCGGAATATAACTTCTACCGGATGGCCGGTATGGCGATTGACGGC
AAAGCCAAGGGCGAAGCCGAAAACGAAGCCAAACCGATCGACGTCAAAGGTCTGTTGCCTTAA

Upstream 100 bases:

>100_bases
TGTCCTTCGGCTGGAAAGTGTGTCTGCCGTTGACGCTGCTGAACCTGCTGGCGACTGCCGCCGTCATTTTGTACAACGCT
CAATAAGGGGTGAATAAACC

Downstream 100 bases:

>100_bases
GGAGCCAGCAAGCATGGAAATTGCCTTTTATCTGGCAGGTTTTATTGCGGTGATCGCGACAATTCGCGTGATCTCGCATA
CCAATCCTGTGCATGCGTTG

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I; NUO9 [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MTLKELLVGFGTQVRSIWMIGLHAFAKRETQMYPEEPVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKA
EQKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEFKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
KAKGEAENEAKPIDVKGLLP

Sequences:

>Translated_180_residues
MTLKELLVGFGTQVRSIWMIGLHAFAKRETQMYPEEPVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKA
EQKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEFKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
KAKGEAENEAKPIDVKGLLP
>Mature_179_residues
TLKELLVGFGTQVRSIWMIGLHAFAKRETQMYPEEPVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAE
QKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEFKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGK
AKGEAENEAKPIDVKGLLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=127, Percent_Identity=40.1574803149606, Blast_Score=102, Evalue=2e-22,
Organism=Escherichia coli, GI1788617, Length=180, Percent_Identity=97.2222222222222, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1789075, Length=146, Percent_Identity=28.0821917808219, Blast_Score=68, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17555194, Length=145, Percent_Identity=40.6896551724138, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17864306, Length=132, Percent_Identity=40.9090909090909, Blast_Score=110, Evalue=5e-25,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20447; Mature: 20316

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKELLVGFGTQVRSIWMIGLHAFAKRETQMYPEEPVYLPPRYRGRIVLTRDPDGEERC
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCCEE
VACNLCAVACPVGCISLQKAEQKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDF
EEEHHHHHHCCCCHHHHHHHHCCCCCCCCHHEEECHHHEEEEEHHHHCCCCCEEEECCCC
EMGEFKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGKAKGEAENEAKPIDVKGLLP
CCCCHHHHCCCEECCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEECCCC
>Mature Secondary Structure 
TLKELLVGFGTQVRSIWMIGLHAFAKRETQMYPEEPVYLPPRYRGRIVLTRDPDGEERC
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCCEE
VACNLCAVACPVGCISLQKAEQKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDF
EEEHHHHHHCCCCHHHHHHHHCCCCCCCCHHEEECHHHEEEEEHHHHCCCCCEEEECCCC
EMGEFKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGKAKGEAENEAKPIDVKGLLP
CCCCHHHHCCCEECCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]