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The map label for this gene is nuoJ [H]

Identifier: 157371537

GI number: 157371537

Start: 3653648

End: 3654208

Strand: Reverse

Name: nuoJ [H]

Synonym: Spro_3300

Alternate gene names: 157371537

Gene position: 3654208-3653648 (Counterclockwise)

Preceding gene: 157371538

Following gene: 157371536

Centisome position: 67.06

GC content: 55.26

Gene sequence:

>561_bases
ATGGAAATTGCCTTTTATCTGGCAGGTTTTATTGCGGTGATCGCGACAATTCGCGTGATCTCGCATACCAATCCTGTGCA
TGCGTTGCTGTATCTGATCGTCTCGTTACTGGCGATCTCTGCAGTCTTTTTCTCACTCGGCGCTTACTTCGCCGCGGCAC
TGGAAATCATTGTCTACGCCGGTGCCATCATGGTGCTGTTCGTGTTCGTGGTGATGATGTTGAACCTCGGCAACGCGGTG
CAGCAACAAGAGCGCGATTGGCTCAAACCGTCGGTGTGGATCGGCCCTGGCATCTTGTCGCTGGCGCTGTTGGTGGTGCT
GATTGTCGCTATCCGCAGCGTGTCCGATCAGGGCATCAGCGGTGAGATGGTTGACGCTAAAGCGGTGGGGATCAGCCTGT
TTGGTCCTTACGTTCTGGCGGTTGAGCTGGCTTCAATGCTGCTGTTGGCAGGTTTGGTTGTTGCCTTCCACATTGGCCGT
GAGCACAAACCGGGTGAGGTGTTGAGCAACGCCCCGGCCGGTAGCGAAATGGCGAGAAGAAAATCGGAGGAGCAAGCATG
A

Upstream 100 bases:

>100_bases
ATGGCCGGTATGGCGATTGACGGCAAAGCCAAGGGCGAAGCCGAAAACGAAGCCAAACCGATCGACGTCAAAGGTCTGTT
GCCTTAAGGAGCCAGCAAGC

Downstream 100 bases:

>100_bases
TCCCTCTTCAACATGGCCTGATCCTGGCGGCAATTCTGTTCGTCCTGGGTCTGACCGGGTTGCTGGTGCGTCGCAATCTG
CTGTTTATGCTGATCAGCCT

Product: NADH dehydrogenase subunit J

Products: NA

Alternate protein names: NADH dehydrogenase I subunit J; NDH-1 subunit J; NUO10 [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MEIAFYLAGFIAVIATIRVISHTNPVHALLYLIVSLLAISAVFFSLGAYFAAALEIIVYAGAIMVLFVFVVMMLNLGNAV
QQQERDWLKPSVWIGPGILSLALLVVLIVAIRSVSDQGISGEMVDAKAVGISLFGPYVLAVELASMLLLAGLVVAFHIGR
EHKPGEVLSNAPAGSEMARRKSEEQA

Sequences:

>Translated_186_residues
MEIAFYLAGFIAVIATIRVISHTNPVHALLYLIVSLLAISAVFFSLGAYFAAALEIIVYAGAIMVLFVFVVMMLNLGNAV
QQQERDWLKPSVWIGPGILSLALLVVLIVAIRSVSDQGISGEMVDAKAVGISLFGPYVLAVELASMLLLAGLVVAFHIGR
EHKPGEVLSNAPAGSEMARRKSEEQA
>Mature_186_residues
MEIAFYLAGFIAVIATIRVISHTNPVHALLYLIVSLLAISAVFFSLGAYFAAALEIIVYAGAIMVLFVFVVMMLNLGNAV
QQQERDWLKPSVWIGPGILSLALLVVLIVAIRSVSDQGISGEMVDAKAVGISLFGPYVLAVELASMLLLAGLVVAFHIGR
EHKPGEVLSNAPAGSEMARRKSEEQA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0839

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 6 (chain J)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 6 family [H]

Homologues:

Organism=Escherichia coli, GI1788616, Length=186, Percent_Identity=74.7311827956989, Blast_Score=273, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001457 [H]

Pfam domain/function: PF00499 Oxidored_q3 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19865; Mature: 19865

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIAFYLAGFIAVIATIRVISHTNPVHALLYLIVSLLAISAVFFSLGAYFAAALEIIVYA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIMVLFVFVVMMLNLGNAVQQQERDWLKPSVWIGPGILSLALLVVLIVAIRSVSDQGIS
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
GEMVDAKAVGISLFGPYVLAVELASMLLLAGLVVAFHIGREHKPGEVLSNAPAGSEMARR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHH
KSEEQA
HHCCCC
>Mature Secondary Structure
MEIAFYLAGFIAVIATIRVISHTNPVHALLYLIVSLLAISAVFFSLGAYFAAALEIIVYA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIMVLFVFVVMMLNLGNAVQQQERDWLKPSVWIGPGILSLALLVVLIVAIRSVSDQGIS
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
GEMVDAKAVGISLFGPYVLAVELASMLLLAGLVVAFHIGREHKPGEVLSNAPAGSEMARR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHH
KSEEQA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]